HKJS_S

Welcome to our Wiki page.

We are students from St. Stephen's College and Carmel Pak U Secondary School. For more than one year, we have worked endlessly on this project. We have read thousands of papers, done hundreds of experiments, spent hours and hours writing an essay. We hope that our efforts aren't futile, and that our project can benefit mankind.

Abstract

Poly(ethyleneterephthalate) (PET) is a prevalent material which can is used in various applications, while bringing adverse effects to the environment. An enzyme, PETase, can degrade the highly-crystalized PET to mono-(2-hydroxyethyl) terephthalic acid (MHET), terephthalate and Bis(2-Hydroxyethyl) terephthalate. MHET is also further decomposed by MHETase to terephthalic acid and ethylene glycol (EG). EG can be further broken down in E. coli K-12 to produce carbon dioxide. Carbon dioxide, however, is a notable greenhouse gas. Using the mutagenesis of the amino acid residues of nifD in nitrogenase, the substrate binding site can be modified so that carbon dioxide can undergo the multi-electron reduction to methane. We propose an efficient carbon dioxide reduction system with the decomposition of PET as the primary carbon source. PETase, MHETase, and amino acid substituted nitrogen fixation genes in MoFe nitrogenase will be expressed in a fast-growing bacterium, E. coli.

For a detailed description of our project. please visit Project Description.

But How is it Changing the World

human practice stuffs

Our Team

Introduce our team