Difference between revisions of "Team:Pasteur Paris/Notebook"
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| − | <div class="description"><p>Transformation of plasmids pET 43.1a and pSB1C3 in E. coli DH5-α competent cells, in order to constitute a stock of empty vectors for our further experiments. </p></div> | + | <div class="description" style="font-size: 100%"><p>Transformation of plasmids pET 43.1a and pSB1C3 in E. coli DH5-α competent cells, in order to constitute a stock of empty vectors for our further experiments. </p></div> |
<div class="daysBoxes tuesday result" id="a17_07_18"> | <div class="daysBoxes tuesday result" id="a17_07_18"> | ||
<a href="">17</a> | <a href="">17</a> | ||
</div> | </div> | ||
| − | <div class="description"><p> | + | <div class="description" style="font-size: 100%"><p>Bacteria transformed with pET43.1a did not grow. Bacteria transformed with pSB1C3 did grow. One colony was placed in liquid culture and we let grow overnight.</p></div> |
<div class="daysBoxes wednesday result" id="a18_07_18"> | <div class="daysBoxes wednesday result" id="a18_07_18"> | ||
<a href="">18</a> | <a href="">18</a> | ||
</div> | </div> | ||
| − | <div class="description"><p> | + | <div class="description" style="font-size: 100%"> |
| + | <p>Midiprep of bacteria containing pSB1C3.<br> | ||
| + | Transformation of our first sequences from Eurofins in E. coli DH5-α: <br> | ||
| + | - 3a_NGF construction Part1 (Seq#1)<br> | ||
| + | - 3a_NGF construction Part2 (Seq#2)<br> | ||
| + | - T7 RIP construction (Seq#8)</p></div> | ||
<div class="daysBoxes thursday result" id="a19_07_18"> | <div class="daysBoxes thursday result" id="a19_07_18"> | ||
<a href="">19</a> | <a href="">19</a> | ||
</div> | </div> | ||
| − | <div class="description"><p> | + | <div class="description" style="font-size: 100%"><p>All the bacteria were successfully transformed with the different plasmids. One colony from each plate was placed in liquid culture and let grow overnight. </p></div> |
<div class="daysBoxes friday result" id="a20_07_18"> | <div class="daysBoxes friday result" id="a20_07_18"> | ||
<a href="">20</a> | <a href="">20</a> | ||
</div> | </div> | ||
| − | <div class="description"><p> | + | <div class="description" style="font-size: 100%"><p> Midiprep of: <br> |
| + | - Seq#1 <br> | ||
| + | - Seq#2 <br> | ||
| + | - Seq#8 </p></div> | ||
<div class="daysBoxes saturday noResult"><a>21</a></div> | <div class="daysBoxes saturday noResult"><a>21</a></div> | ||
<div class="daysBoxes sunday noResult"><a>22</a></div> | <div class="daysBoxes sunday noResult"><a>22</a></div> | ||
Revision as of 16:18, 29 September 2018
Transformation of plasmids pET 43.1a and pSB1C3 in E. coli DH5-α competent cells, in order to constitute a stock of empty vectors for our further experiments.
Bacteria transformed with pET43.1a did not grow. Bacteria transformed with pSB1C3 did grow. One colony was placed in liquid culture and we let grow overnight.
Midiprep of bacteria containing pSB1C3.
Transformation of our first sequences from Eurofins in E. coli DH5-α:
- 3a_NGF construction Part1 (Seq#1)
- 3a_NGF construction Part2 (Seq#2)
- T7 RIP construction (Seq#8)
All the bacteria were successfully transformed with the different plasmids. One colony from each plate was placed in liquid culture and let grow overnight.
Midiprep of:
- Seq#1
- Seq#2
- Seq#8