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Cytotoxic anticancer drugs are among the harmful chemicals found in hospital wastewater at high concentrations. Degradation through physical and chemical methods exist but are often inefficient, unsustainable or expensive. We propose MethotrExit, a bioreactor-based approach to tackle this problem. We focused on the biotransformation of methotrexate (MTX), a widely used anticancer drug. We designed synthetic cassettes encoding a new biotransformation pathway using a heterologous carboxypeptidase in Escherichia coli. In only five hours, MethotrExit drastically removes MTX from the media. However, anticancer drug degradation products and/or the biotransformation pathway itself might be toxic for E. coli. To overcome this issue, biobricks generating heterogeneity in enzyme expression were built to ensure survival of a subpopulation. Modeling of this system highlights the interest of a division of labor between “cleaning” and “stem” bacterial cells.
Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
This year IGEM Bordeaux Team would like to find an alternative to an entire segment of the traditional petrobased chemistry by a new green biobased chemistry. Indeed, we would like to focus on the biocatalysis of the hydroxymethylfurfural (HMF) in 2,5-furandicarboxylic acid (FDCA). Don’t worry, it is not as complicated as it appears. HMF is a by-product of the lignocellulosic biomass treatment. Its toxicity toward microorganisms leads to big issue for many companies which want to use these microorganisms to produce molecules of interest from lignocellulosic biomass. Our project consists in HMF detoxification by using it as a substrate to produce FDCA through bacteria .FDCA was identify as one of most promising biobased molecules which can replace many polymers such as PET (and other petrobased molecules). We suggest a sustainable alternative, eco-friendly and independent from fossil resource.
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Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
iGEM Newcastle
iGEM Newcastle
Alternative Roots: Engineering Microbial Communities
Abtract
The demand for food, fuel and materials is placing unprecedented pressure on agricultural production. To secure higher productivity, the sector relies upon synthetic fertilisers derived from energy intensive manufacturing methods. Here, we propose an alternative approach to support plant productivity. The Alternative Roots project investigated Pseudomonas fluorescens as a chassis organism. Development of a plant-colonising chassis provides novel mechanisms for soil microbiome manipulation without genetically modified crops. As proof of concept, we focus on improving nitrogen supply via naringenin biosynthesis - a potential chemoattractant of free-living, nitrogen-fixing bacteria. Legal and social considerations of the project drove the development of NH-1, a low-cost, small-scale and programmable hydroponic system. Tailored to overcome experimental limitations faced by many plant scientists, NH-1 provides improved reproducibility, coupled with high-throughput experimentation. This system enabled exploration of future deployment techniques within contained environments that may result in enhanced, sustainable crop productivity at a local and accessible level.
Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
iGEM Montpellier
iGEM Montpellier
Vagineering : A New Non Hormonal Contraception
Abtract
Modern hormonal contraceptive methods have been revolutionary for women in developed countries; however, they still exhibit a variety of challenges. Developing countries lack consistent access, hormonal contraceptives can produce harmful environmental effects, and some women are unable use them due to health problems. The Vagineering project looks to solve these issues with a novel, non-hormonal method. Our team aims to engineer Lactobacillus jensenii, a bacterium from the vaginal flora, to produce two proteins to prevent unintentional pregnancy: antisperm antibodies that inhibit sperm motility and anti-microbial peptides (AMPs) that produce spermicidal effects. The goal is to create a lasting contraceptive using only bacteria, which can later be reversed by engineering the strain with a kill-switch. Additionally, our studies of this strain have produced a toolbox that will help other teams to further engineer this less-characterized bacterium.
Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
iGEM IIT Madras
iGEM IIT Madras
ADaPtat1on : Expanding Toolkit for Acinetobacter baylyi
Abtract
Acinetobacter baylyi is a gram-negative, soil-dwelling, non-pathogenic, naturally competent and nutritionally versatile organism especially known for its ability to degrade aromatic compounds. However, only a few tools are available for its gene manipulation. This year, we plan to expand the toolkit for A. baylyi ADP1 by making a synthetic promoter library along with codon optimized fluorescent reporter proteins to achieve better control over its expression rates. The codon table is not available for this organism. So we obtained sequence data of well-characterised proteins of this organism by filtering manually putative and hypothetical sequences and used this data to generate the codon table using CUTE - a tool of ChassiDex. The codon optimisation is done manually by replacing the less frequent codons with high-frequency codons based on the generated table. This can potentially open up various new exciting synthetic biology opportunities with this unexplored organism.
Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
iGEM Duesseldorf
iGEM Duesseldorf
Trinity - towards an engineered co-culture toolbox
Abtract
Co-cultures are found in all conceivable entities, such as the human gut, cheese or plants, but good tools to study those communities are currently not given. Indeed we created a modularly built toolbox using not only three different dependencies but also three different organisms: With Escherichia coli, Saccharomyces cerevisiae and Synechococcus elongatus our team engineered a system based on nutrient exchange. Here phosphate is provided through oxidation of phosphite, nitrogen source produced by melamine breakdown, whilst carbon source is provided by Synechococcus elongatus. Two additional independent approaches are designed, too. The first includes regulation via cross-feeding by amino acid auxotrophies and production: lysine by Escherichia coli and leucine by Saccharomyces cerevisiae. The other utilizes regulated self-lysis via quorum sensing molecules, to control cell density by a phage lysis gene. This engineered toolbox opens a wide range of possibilities to create microbial communities for different purposes, such as synthetic probiotics.
Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
iGEM Pasteur
iGEM Pasteur
NeuronArch: the novel connecting and protecting biofilm based system for prostheses
Abtract
In the future, a long due consideration and an easier access to healthcare will be given to people with disabilities. Presently, some prostheses allow amputees to perform simple actions but without a direct connection between the nerves and the prosthesis. Furthermore, a major health risk is the development of pathogenic communities of microorganisms in structures called biofilms. Strong treatments with antibiotics, or even surgical reinterventions are then required. They represent a heavy burden for both the patient and the healthcare system. We imagined NeuronArch as a novel application that subverts potential pathogenic biofilms using an engineered one. This interface produces substances called neurotrophins (NGF), for directed and controlled growth of nerves. Using a conductive membrane, it will also allow passing of information and enhancement of the electrical properties. Altogether, these improvements would enable patients to regain natural perceptions and prevent the formation of Staphylococcus aureus biofilms by blocking quorum sensing.
Haec subinde Constantius audiens et quaedam referente Thalassio doctus, quem eum odisse iam conpererat lege communi, scribens ad Caesarem blandius adiumenta paulatim illi subtraxit, sollicitari se simulans ne, uti est militare otium fere tumultuosum, in eius perniciem conspiraret, solisque scholis iussit esse contentum palatinis et protectorum cum Scutariis et Gentilibus, et mandabat Domitiano, ex comite largitionum, praefecto ut cum in Syriam venerit, Gallum, quem crebro acciverat, ad Italiam properare blande hortaretur et verecunde.
