Difference between revisions of "Team:Grenoble-Alpes/construction"

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<p id="titre-image">PHAGE LYSIS & DNA EXTRACTION</p>
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<p id="titre-image">PROBE CONSTRUCTION</p>
 
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<h2><center><font color="blue">PSEUDOMONAS AERUGINOSA DETECTION</font></center></h2>
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<h3><font size="5"><font color="blue">LYSIS</font></font></h3>
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<h3><font size="4">1. Plasmid backbone selection</font></h3>
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<p>Among all the backbones proposed by iGEM, we decided to choose one for our probe insertion. In fact, the 2017 IGEM Grenoble team showed by their experiments, that the pSB1C3-BBa_J04450 backbone was the most suitable for the detection of a red fluorescence.</p>
  
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<figure><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Grenoble-Alpes--select_figure4.png"><figcaption>Figure 1: Backbone tested for hybridization of the target to the probe detecting bacterial lysis  </figcaption></figure>
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Revision as of 20:37, 8 October 2018

Template loop detected: Template:Grenoble-Alpes

PROBE CONSTRUCTION

PSEUDOMONAS AERUGINOSA DETECTION


LYSIS


1. Plasmid backbone selection


Among all the backbones proposed by iGEM, we decided to choose one for our probe insertion. In fact, the 2017 IGEM Grenoble team showed by their experiments, that the pSB1C3-BBa_J04450 backbone was the most suitable for the detection of a red fluorescence.


Figure 1: Backbone tested for hybridization of the target to the probe detecting bacterial lysis