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− | <h2><center><font color=" | + | <h2><center><font color="#0066cc">PSEUDOMONAS AERUGINOSA DETECTION</font></center></h2> |
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<h3><font size="4">1. Plasmid backbone selection</font></h3> | <h3><font size="4">1. Plasmid backbone selection</font></h3> | ||
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<p>Among all the backbones proposed by iGEM, we decided to choose one for our probe insertion. In fact, the 2017 IGEM Grenoble team showed by their experiments, that the pSB1C3-BBa_J04450 backbone was the most suitable for the detection of a red fluorescence.</p> | <p>Among all the backbones proposed by iGEM, we decided to choose one for our probe insertion. In fact, the 2017 IGEM Grenoble team showed by their experiments, that the pSB1C3-BBa_J04450 backbone was the most suitable for the detection of a red fluorescence.</p> | ||
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− | <figure><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Grenoble-Alpes--select_figure4.png"><figcaption>Figure 1: Backbone tested for hybridization of the target to the probe detecting bacterial lysis </figcaption></figure> | + | <figure><center><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Grenoble-Alpes--select_figure4.png"><figcaption>Figure 1: Backbone tested for hybridization of the target to the probe detecting bacterial lysis </figcaption></center></figure> |
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Revision as of 20:39, 8 October 2018
Template loop detected: Template:Grenoble-Alpes
PROBE CONSTRUCTION
PSEUDOMONAS AERUGINOSA DETECTION
LYSIS
1. Plasmid backbone selection
Among all the backbones proposed by iGEM, we decided to choose one for our probe insertion. In fact, the 2017 IGEM Grenoble team showed by their experiments, that the pSB1C3-BBa_J04450 backbone was the most suitable for the detection of a red fluorescence.