Difference between revisions of "Team:Madrid-OLM/AptamerProtocols"
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<li class="nomargin"><p class="lead">Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.</p></li> | <li class="nomargin"><p class="lead">Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.</p></li> | ||
<li class="nomargin"><p class="lead">To get rid of the DNA that unespecifically binds to the system, apply the library through a nitrocellulose membrane and centrifuge 1 min at 8000 rpm. Quantify the DNA that does not bind unspeficically and note it as the initial DNA.</p></li> | <li class="nomargin"><p class="lead">To get rid of the DNA that unespecifically binds to the system, apply the library through a nitrocellulose membrane and centrifuge 1 min at 8000 rpm. Quantify the DNA that does not bind unspeficically and note it as the initial DNA.</p></li> | ||
| − | < | + | <h4>Prepare the library pool</h4> |
<li class="nomargin"> <p class="lead">Resuspend 2 nmol de la library pool on 200 µl of Binding Buffer (BB).</p></li> | <li class="nomargin"> <p class="lead">Resuspend 2 nmol de la library pool on 200 µl of Binding Buffer (BB).</p></li> | ||
<li class="nomargin"><p class="lead">Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.</p></li> | <li class="nomargin"><p class="lead">Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.</p></li> | ||
Revision as of 12:42, 10 October 2018
Aptamer's Protocols
Texto de explicacion/ resumen de la pagina.