Difference between revisions of "Team:Grenoble-Alpes/temperature module"
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| + | <p>In the automated system, many steps require to heat or cool biological samples. Typically, the biological transformation is a good example: the samples containing the bacteria and the DNA need to be heated and cooled for precise durations at a constant temperature to realize a heat shock (a classical transformation goes from 0°C for 30 minutes to 42°C for 1 minute and back at 0°C for 2 minutes). Hence, this module has to be carefully considered because it is a key point to the success of any biological manipulation. In addition, because our system will be fully automated and nobody will be able to check the inside of the machine while it works, a reliable and robust module is required so that each time it is used, repeatable results are obtained.</p><p> | ||
| + | In the following figure, the different biological steps are summarized and their required temperatures are given.</p> | ||
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| + | <figure><center><img src="https://static.igem.org/mediawiki/2018/8/83/T--Grenoble-Alpes--temperatureFig1.png" style="width:70vh"><figcaption>Figure 1: Summary of the required temperatures in the system </figcaption></center></figure> | ||
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| + | <p>By design, each biological step will be contained in a module with a given temperature. This allows us to make temperature shocks by pipetting the liquid inside an Eppendorf tube from one place with a given temperature (0°C for instance) to another place in the machine with another temperature (42°C this time).</p> | ||
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| + | <h2><font color=#93024b><center>Light pathway through the fluorescence sensor</center></font></h2> | ||
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| + | <figure><center><img src="https://static.igem.org/mediawiki/2018/c/c9/T--Grenoble-Alpes--temperatureFig2.png" style="width:70vh"><figcaption>Figure 2: Schematic of the temperature regulation loop (IR = Infra-red) and photography of the cooling module </figcaption></center></figure> | ||
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Revision as of 08:21, 12 October 2018
Template loop detected: Template:Grenoble-Alpes
TEMPERATURE MODULE
In the automated system, many steps require to heat or cool biological samples. Typically, the biological transformation is a good example: the samples containing the bacteria and the DNA need to be heated and cooled for precise durations at a constant temperature to realize a heat shock (a classical transformation goes from 0°C for 30 minutes to 42°C for 1 minute and back at 0°C for 2 minutes). Hence, this module has to be carefully considered because it is a key point to the success of any biological manipulation. In addition, because our system will be fully automated and nobody will be able to check the inside of the machine while it works, a reliable and robust module is required so that each time it is used, repeatable results are obtained.
In the following figure, the different biological steps are summarized and their required temperatures are given.
By design, each biological step will be contained in a module with a given temperature. This allows us to make temperature shocks by pipetting the liquid inside an Eppendorf tube from one place with a given temperature (0°C for instance) to another place in the machine with another temperature (42°C this time).
Light pathway through the fluorescence sensor
