Difference between revisions of "Team:Tianjin/Safety"

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                    <li><a href="https://2018.igem.org/Team:Tianjin/Description">DESCRIPTION</a></li>
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                    SAFETY             
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                <p>
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              Although our project barely involves complex genetic pollution or the use of toxic reagents, our instructor still attaches great importance to the lab safety during the experiment. Before entering the lab, every operator must accept comprehensive training and have a deep understanding of equipment and reagents. All wastes are divided and gathered into special bottles, later get handled by professionals in a unified manner. In our activities like open day and program shooting, we also paid close attention on the safety of visitors and guided them to use the appliance correctly.
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                    <p>Safety Training</p>
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                <p>
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              <i class="fa fa-circle"></i>Before we start doing experiments, our teacher first held a systematic safety training, which contains the safe use of experimental apparatus and other laboratory safety rules. Only when we had qualified awareness of lab safety and security can we do the experiments ourselves.>
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                <p>
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                  <i class="fa fa-circle"></i>To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.
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                  <i class="fa fa-circle"></i>We have weekly meetings to summarize the problems when doing experiments last week and came up with solutions. During the meetings, students would sum up the mistakes and remind each other of the safety precautions.
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                    <p>General laboratory safety</p>
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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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                    <p>1.Equipment safety </p>
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                  <i class="fa fa-circle"></i>To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.
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                <p>
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                  <i class="fa fa-circle"></i>To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.
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                <p>
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                  <i class="fa fa-circle"></i>To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.
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                <p>
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                  <i class="fa fa-circle"></i>To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.
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                    <p>2.Personal and operational safety </p>
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                  <i class="fa fa-circle"></i>Before the experiment, we should carefully study the use of each instrument and get to know how to deal with the dangerous chemicals.
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                  <i class="fa fa-circle"></i>We should watch out during operation to avoid the failure of instrument and the consequent damage.
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                  <i class="fa fa-circle"></i>We must not eat or drink in the lab.
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                <p>
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                  <i class="fa fa-circle"></i>We should wear disposable gloves and avoid touching our mouth, eyes and face during operation.
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                  <i class="fa fa-circle"></i>It’s forbidden to take any instruments away from our laboratory.
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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                    <p>Specific reagents safety</p>
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              We set up a special room for gel electrophoresis and other similar toxic experiments. All things in this room are not allowed to be touched without protection or taken out. We investigate the hazardous reagents that may involve and the property information is posted in the lab:
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                                1. Acrylamide:
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                                      The results of the acute toxicity test indicate that, in mice, guinea pigs, and rabbits, the LD50 is 150 to 180 mg/kg, which is a medium toxic substance. Animal studies have found that acrylamide can cause various tumors of organs in rats, including breast, thyroid, testicles, adrenal gland, central nervous system, mouth, uterus, pituitary gland and so on. In 1994, IARC evaluated its carcinogenicity, classifying acrylamide as a class 2 carcinogen (2A), a possible human carcinogen, mainly based on the fact that acrylamide can be metabolized into its carcinogenic active metabolite epoxy propanamide in both animals and humans.
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                                2. TEMED:
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                                    <p>
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                                      Inflammable, volatile, corrosive, strong neurotoxicity, in order to avoid inhaling, it is supposed to use the rear cover to tighten the bottle cap.
 +
                                    </p>
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                                </div>
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<h3>Safe Project Design</h3>
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                                3. Ammonium persulfate:
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                            </a>
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                                    <p>
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                                      It is irritant and corrosive to skin mucous membrane. Once inhaling ,it may cause rhinitis, laryngitis, shortness of breath and coughing. What’s more , it can cause intense irritation, pain and even burns after contact with eyes and skin. Oral administration causes abdominal pain, nausea and vomiting. Long-term exposure to skin contact can cause allergic dermatitis.                                    </p>
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                </div>             
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            </div>
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        </div>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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                                4. SDS：
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                            </a>
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                                    <p>
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                                      It can stimulate mucosa and upper respiratory tract, as well as eye and skin. It also can cause allergic reaction of respiratory system.β-mercaptoethanol (BME)：Inhalation, ingestion or absorption of BME through the skin will be toxic. The symptoms include cyanosis, vomiting, tremor, headache, convulsions, coma, and even death. It also has strong irritation to eyes and skinwhich may cause corneal opacity.
 +
                                    </p>
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                                </div>
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                        </div>
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            </div>
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        </div>
  
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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                    <div class="pan-heading text-pan-heading" id="collapsehead">
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<li>Including an "induced lethality" or "kill-switch" device</li>
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                                5. Methanol:
 +
                            </a>
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                        </div>
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                                    <p>
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                                      Methanol toxicity has the greatest impact on the nervous system and blood system of the human body. It can cause toxic reactions through the digestive tract, respiratory tract or skin intake. Methanol vapor can damage the respiratory mucosa and eyesight of human. However, it is a low toxicity reagents.
 +
                                    </p>
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                                </div>
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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                                6. Glacial acetic acid:
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                            </a>
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<h3>Safe Shipment</h3>
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                        </div>
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                    </div>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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                                      It is irritant to nose, throat and respiratory tract after inhalation. It has a strong irritating effect on the eye. Its contact with skin can lead to erythema, even causing chemical burns.All toxic items mentioned above will be collected and specially treated.
 +
                                    </p>
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Revision as of 10:19, 13 October 2018

<!DOCTYPE html> Team:Tianjin - 2018.igem.org

Although our project barely involves complex genetic pollution or the use of toxic reagents, our instructor still attaches great importance to the lab safety during the experiment. Before entering the lab, every operator must accept comprehensive training and have a deep understanding of equipment and reagents. All wastes are divided and gathered into special bottles, later get handled by professionals in a unified manner. In our activities like open day and program shooting, we also paid close attention on the safety of visitors and guided them to use the appliance correctly.

Safety Training

Before we start doing experiments, our teacher first held a systematic safety training, which contains the safe use of experimental apparatus and other laboratory safety rules. Only when we had qualified awareness of lab safety and security can we do the experiments ourselves.>

To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.

We have weekly meetings to summarize the problems when doing experiments last week and came up with solutions. During the meetings, students would sum up the mistakes and remind each other of the safety precautions.

General laboratory safety

1.Equipment safety

To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.

To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.

To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.

To ensure that everyone does the experiments in a safe and proper manner, our adviser set up a rule that everyone must complete the experiment under the supervision of another student. In this way, when there is a safety emergency, students can get immediate help from others.

2.Personal and operational safety

Before the experiment, we should carefully study the use of each instrument and get to know how to deal with the dangerous chemicals.

We should watch out during operation to avoid the failure of instrument and the consequent damage.

We must not eat or drink in the lab.

We should wear disposable gloves and avoid touching our mouth, eyes and face during operation.

It’s forbidden to take any instruments away from our laboratory.

Specific reagents safety

We set up a special room for gel electrophoresis and other similar toxic experiments. All things in this room are not allowed to be touched without protection or taken out. We investigate the hazardous reagents that may involve and the property information is posted in the lab:

1. Acrylamide:
2. TEMED:
3. Ammonium persulfate:
4. SDS：
5. Methanol:
6. Glacial acetic acid: