Difference between revisions of "Team:Jilin China/Result/Version 2"
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<p>After the integrated human practice work, we update SynRT Toolkit to version 2.0. In version 2.0, we added 23 heat-repressible RNA-based thermosensors to Toolkit.</p> | <p>After the integrated human practice work, we update SynRT Toolkit to version 2.0. In version 2.0, we added 23 heat-repressible RNA-based thermosensors to Toolkit.</p> | ||
<p>Heat-repressible RNA-based thermosensors were based on the RNase E. We measured these thermosensors' activity by using measurement device like before. But this time, we designed a new negative control. In the following, it will be called negative control-2. Negative control-2 has a cleavage site of RNase E. It will always be digest by enzyme. Due to the effiency of RNase E, we decided to use negative control-2 instead of traditional negative control.</p> | <p>Heat-repressible RNA-based thermosensors were based on the RNase E. We measured these thermosensors' activity by using measurement device like before. But this time, we designed a new negative control. In the following, it will be called negative control-2. Negative control-2 has a cleavage site of RNase E. It will always be digest by enzyme. Due to the effiency of RNase E, we decided to use negative control-2 instead of traditional negative control.</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/c/cc/T--Jilin_China--result--REbar.png" | + | <img src="https://static.igem.org/mediawiki/2018/c/cc/T--Jilin_China--result--REbar.png" width="95%" length="95%"></img> |
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Revision as of 18:09, 13 October 2018
VERSION 2.0
-
Results
After the integrated human practice work, we update SynRT Toolkit to version 2.0. In version 2.0, we added 23 heat-repressible RNA-based thermosensors to Toolkit.
Heat-repressible RNA-based thermosensors were based on the RNase E. We measured these thermosensors' activity by using measurement device like before. But this time, we designed a new negative control. In the following, it will be called negative control-2. Negative control-2 has a cleavage site of RNase E. It will always be digest by enzyme. Due to the effiency of RNase E, we decided to use negative control-2 instead of traditional negative control.
