Difference between revisions of "Team:Jilin China/Result/Version 3"

(Created page with "{{:Team:Jilin_China/CSS}} {{:Team:Jilin_China/CSS_Details}} {{:Team:Jilin_China/Navbar}} {{:Team:Jilin_China/Js}} <html><!--For CSS and Js--> <style> .s1{ background: url(htt...")
 
Line 50: Line 50:
 
<section class="s1">
 
<section class="s1">
 
   <div class="title_con">
 
   <div class="title_con">
−
   <p><span>TOOLKITS</span><br>VERSION 13.0</p>
+
   <p><span>TOOLKITS</span><br>VERSION 3.0</p>
 
   <br />
 
   <br />
 
   <table>
 
   <table>
Line 70: Line 70:
 
     <li class="pragraph_1 start" id="pragraph_1">
 
     <li class="pragraph_1 start" id="pragraph_1">
 
     <div>
 
     <div>
−
       <h2>Title</h2>
+
       <h2>Results</h2>
 +
<p>We added 10 cold-reprssible RNA-based thermosensors and 8 cold-inducible RNA-based thermosensors to the SynRT Toolkit. After that, we named the updated toolkit as SynRT Toolkit version 3.0.</p>
 +
<h3>Cold-repressible RNA-based thermosensors</h3>
 +
<p>Cold-repressible RNA-based thermosensors are designed based on the RNase III. We constructed their measurement devices and measured the activities at five temperatures: 15, 25, 29, 35 and 37℃. The measured temperature range is lower than the heat-inducible RNA-based thermosensors' temperature range. We also designed a new negtive control, which always has a cleavage site of RNase III, so it will be digest by the enzyme. After measurement, we chose 10 out of 50 thermosensors. </p>
 +
<p>Figure 1 shows the change of activities of different thermosensors. We find that all of these thermosensors' activities increase with temperature increasing. </p>
 +
<img src="https://static.igem.org/mediawiki/2018/4/48/T--Jilin_China--result--R3bar.png" width="95%"></img>
 +
<p>For most of the cold-repressible RNA-based thermosensors, the change of activities from 15 to 25℃ is noticeable than 25 to 35℃. And the melting temperature is lower than the heat-inducible RNA thermosensors. Figure 2 shows the fold-change of activity in different temperature. </p>
 +
<img src="https://static.igem.org/mediawiki/2018/4/48/T--Jilin_China--result--R3bar.png" width="95%"></img>
 
     </div>
 
     </div>
 
     </li>
 
     </li>

Revision as of 04:50, 14 October 2018

TOOLKITS
VERSION 3.0


Promoters

VERSION 3.0

  • Results

    We added 10 cold-reprssible RNA-based thermosensors and 8 cold-inducible RNA-based thermosensors to the SynRT Toolkit. After that, we named the updated toolkit as SynRT Toolkit version 3.0.

    Cold-repressible RNA-based thermosensors

    Cold-repressible RNA-based thermosensors are designed based on the RNase III. We constructed their measurement devices and measured the activities at five temperatures: 15, 25, 29, 35 and 37℃. The measured temperature range is lower than the heat-inducible RNA-based thermosensors' temperature range. We also designed a new negtive control, which always has a cleavage site of RNase III, so it will be digest by the enzyme. After measurement, we chose 10 out of 50 thermosensors.

    Figure 1 shows the change of activities of different thermosensors. We find that all of these thermosensors' activities increase with temperature increasing.

    For most of the cold-repressible RNA-based thermosensors, the change of activities from 15 to 25℃ is noticeable than 25 to 35℃. And the melting temperature is lower than the heat-inducible RNA thermosensors. Figure 2 shows the fold-change of activity in different temperature.