Difference between revisions of "Team:Pasteur Paris/Medals"
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<td><b>Validated Part / Validated Contribution</b></td> | <td><b>Validated Part / Validated Contribution</b></td> | ||
| − | <td>We created a new BioBrick part as expected : <a href="http://parts.igem.org/Part:BBa_K2616000" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">BBa_K2616000</a>. This part permits to | + | <td>We created a new BioBrick part as expected : <a href="http://parts.igem.org/Part:BBa_K2616000" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">BBa_K2616000</a>. This part permits to express pro-NGF and normally, secrete it in the extracellular medium using E. coli type I secretion system. We used inducible promoter T7, in order to control pro-NGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. pro-NGF is addressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacide of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated pro-NGF from this 60 aminoacid long sequence by a cleaving site for TEV protease Nter-ENLYFQ-Cter. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. We succeeded in characterizing our pro-NGF by sequencing and mass spectrometry but could not purify it from the media. </td> |
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<td><b> Collaboration </b</td> | <td><b> Collaboration </b</td> | ||
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| − | We hosted the <a href="https://2018.igem.org/Team:Pasteur_Paris/Collaborations#MeetUp"style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">4th Parisian Meet-Up </a>where we | + | We hosted the <a href="https://2018.igem.org/Team:Pasteur_Paris/Collaborations#MeetUp"style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">4th Parisian Meet-Up </a>where we organized a Tiny Jamboree in the morning in order to practice with all the French teams for the Giant. We also organized and attended round tables about bioethics with multiples professionals during the afternoon.</br> |
| − | We collaborated with other teams, | + | We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this <a href="https://2018.igem.org/Team:Pasteur_Paris/Collaborations" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank"> <b> page </b> </a>.</br> |
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Revision as of 14:41, 14 October 2018
Bronze medal criteria
| Explanation | Criteria achieved |
| Registration and Giant Jamboree Attendance | We registered for iGEM then had a great summer time. We are now waiting to attend the Giant Jamboree. |
| Competition Deliverables | We plan to make the competition deliverables met on time. |
| Attributions | We completed the attributions page on our wiki. |
| Characterization / Contribution | We successfully completed the InterLab Measurement Study. |
Silver medal criteria
| Explanation | Criteria achieved |
| Validated Part / Validated Contribution | We created a new BioBrick part as expected : BBa_K2616000. This part permits to express pro-NGF and normally, secrete it in the extracellular medium using E. coli type I secretion system. We used inducible promoter T7, in order to control pro-NGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. pro-NGF is addressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacide of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated pro-NGF from this 60 aminoacid long sequence by a cleaving site for TEV protease Nter-ENLYFQ-Cter. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. We succeeded in characterizing our pro-NGF by sequencing and mass spectrometry but could not purify it from the media. |
| Collaboration | We hosted the 4th Parisian Meet-Up where we organized a Tiny Jamboree in the morning in order to practice with all the French teams for the Giant. We also organized and attended round tables about bioethics with multiples professionals during the afternoon. We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this page . |
| Human Practices | Ethics and safety: Security: The Institut Pasteur is a highly restricted area so we had ID badges to enter every building and unity. + substances we used Sustainability: ? Kill switch ? Social justice: ? Intellectual property rights: account of state of the art, no infrigement of IP laws. |
Gold medal criteria
| Explanation | Criteria achieved |
| Integrated Human Practices | Integrated HP |
| Improve a Previous Part or Project | |
| Model Your Project | We modelled how NGF is produced in our modified E. Coli, its diffusion in an environment and its consequences on neurons growth. It helped to predict how to use NGF in our experiments. |