Difference between revisions of "Team:Pasteur Paris/Medals"

Revision as of 14:47, 14 October 2018

Bronze medal criteria

Explanation	Criteria achieved
Registration and Giant Jamboree Attendance	We registered for iGEM then had a great summer time. We are now waiting to attend the Giant Jamboree.
Competition Deliverables	We plan to make the competition deliverables met on time.
Attributions	We completed the attributions page on our wiki.
Characterization / Contribution	We successfully completed the InterLab Measurement Study.

Silver medal criteria

Explanation	Criteria achieved
Validated Part / Validated Contribution	We created a new BioBrick part as expected : BBa_K2616000. This part permits to express pro-NGF and normally, secrete it in the extracellular medium using E. coli type I secretion system. We used inducible promoter T7, in order to control pro-NGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. pro-NGF is addressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacide of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated pro-NGF from this 60 aminoacid long sequence by a cleaving site for TEV protease Nter-ENLYFQ-Cter. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. We succeeded in characterizing our pro-NGF by sequencing and mass spectrometry but could not purify it from the media.
Collaboration	We hosted the 4th Parisian Meet-Up where we organized a Tiny Jamboree in the morning in order to practice with all the French teams for the Giant. We also organized and attended round tables about bioethics with multiples professionals during the afternoon. We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this page .
Human Practices	Ethics and safety: Security: The Institut Pasteur is a highly restricted area so we had ID badges to enter every building and unity. + substances we used Sustainability: ? Kill switch ? Social justice: ? Intellectual property rights: account of state of the art, no infrigement of IP laws.

Gold medal criteria

Explanation	Criteria achieved
Integrated Human Practices	Integrated HP
Improve a Previous Part or Project
Model Your Project	We modelled how NGF is produced in our modified E. Coli, its diffusion in an environment and its consequences on neurons growth. It helped to predict how to use NGF in our experiments.

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 We hosted the <a href="https://2018.igem.org/Team:Pasteur_Paris/Collaborations#MeetUp"style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">4th Parisian Meet-Up </a>where we organized a Tiny Jamboree in the morning in order to practice with all the French teams for the Giant. We also organized and attended round tables about bioethics with multiples professionals during the afternoon.</br>
-We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this <a href="https://2018.igem.org/Team:Pasteur_Paris/Collaborations" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank"> <b> page </b> </a>.</br>
+We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this <a href="https://2018.igem.org/Team:Pasteur_Paris/Collaborations" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank"> page </a>.</br>
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