Difference between revisions of "Team:Madrid-OLM/Hardware"

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                             <p class="lead">The environment that motivated the birth of the final prototype was a different one. We would love to consider this device as a mature version of the initial one. We introduced the following improvements: </p>
 
                             <p class="lead">The environment that motivated the birth of the final prototype was a different one. We would love to consider this device as a mature version of the initial one. We introduced the following improvements: </p>
 
                             <ol class="ourlist">
 
                             <ol class="ourlist">
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                                 <li class="nomargin"><p class="lead">Arduino’s M7 diode, which job is to avoid an eventual situation of reverse current, has been removed. This is because of his inability to stand the 4 amperes that go through the system when the 8 motors are at their full capacity..</p></li>
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                                 <li class="nomargin"><p class="lead">Electrochemical system of measurement: we required a potentiostat and some electrodes for testing the protein and aptamers binding. </p></li>
−
                                 <p class="lead nomargin">In its position, we have solder a IRLZ44N transistor, able to stand up to 50 A. To do it, the pins of the source and drain were connected in a similar way as the pins of the diode and the gate pin was connected to the 12V  power supply. A heat sink was also put in the upper side.</p>
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                                 <li class="nomargin"><p class="lead">Integrate an electrode into a microfluidic chip.</p></li>
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                                 <li><p class="lead">An Arduino Shield was mounted to increase the total of pins to 8 Vin an 8 GND, to connects the power of the motor drivers.</p></li>
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                                <li class="nomargin"><p class="lead">Correlate the protein concentration to the potentiostat readings.</p></li>
 +
                                <li class="nomargin"><p class="lead">Automated pressure pump control for the microfluidic chip.</p></li>
 +
                                 <li class="nomargin"><p class="lead">Capability of real-time data uploading to the cloud.</p></li>
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                                <li class="nomargin"><p class="lead">Integrate this data in an iOS app.</p></li>
 +
                                <li><p class="lead">Integrate every subsystem into a functional device.</p></li>
 
                             </ol>
 
                             </ol>
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                             <p class="lead">Besides, we would love to consider that our final version of the device is versatile enough to be presented as a resilient biodevice workbench. It might be used by anyone that requires a device for experimenting with aptasensors based on an electrochemical system of measurement.</p> 
−
                               
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                            <p class="lead">Although we are presenting a design that seems to be closed, we are proud to share with everyone our design in <a href="http://github.com/OpenLabMadrid/iGEM-Madrid-OLM/tree/master/CAD/Second%20Prototype">our Github</a>. It is open to improvements. We would love to receive as much feedback as possible. </p>
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                             <p class="lead">The results demonstrate the capacity of our electrode to change the voltage proportionally to the bound thrombin. We could establish a mathematical model which correlates the voltage drop (compared to a negative control) to the thrombin concentration in the sample.</p>
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                         </div>
 
                         </div>
 
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Revision as of 09:35, 15 October 2018

Madrid-OLM

Overview final device

Final device

Our second device was born from a need. It was not preconceived, as the first prototype. We needed to solve many experimental inconveniences and many misconceptions that we had when we designed the first prototype.

First Prototype

The environment that motivated the birth of the final prototype was a different one. We would love to consider this device as a mature version of the initial one. We introduced the following improvements:

  1. Electrochemical system of measurement: we required a potentiostat and some electrodes for testing the protein and aptamers binding.

  2. Integrate an electrode into a microfluidic chip.

  3. Correlate the protein concentration to the potentiostat readings.

  4. Automated pressure pump control for the microfluidic chip.

  5. Capability of real-time data uploading to the cloud.

  6. Integrate this data in an iOS app.

  7. Integrate every subsystem into a functional device.

Besides, we would love to consider that our final version of the device is versatile enough to be presented as a resilient biodevice workbench. It might be used by anyone that requires a device for experimenting with aptasensors based on an electrochemical system of measurement.

Although we are presenting a design that seems to be closed, we are proud to share with everyone our design in our Github. It is open to improvements. We would love to receive as much feedback as possible.