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<p class="lead">During this competition, we have faced a different kind of problems, but the principal ones were the lack of money and time. We have felt the absence of resources in both of the parts of our project, the aptamer development, of the device manufacturing. For solving this issue we have learned to make all the equipment that we had need during the project by ourselves, getting the inspiration in the DIYBio movement. Now we are proud to contribute to the iGEM community with a new successful way of:</p> | <p class="lead">During this competition, we have faced a different kind of problems, but the principal ones were the lack of money and time. We have felt the absence of resources in both of the parts of our project, the aptamer development, of the device manufacturing. For solving this issue we have learned to make all the equipment that we had need during the project by ourselves, getting the inspiration in the DIYBio movement. Now we are proud to contribute to the iGEM community with a new successful way of:</p> | ||
<ol class="ourlist"> | <ol class="ourlist"> | ||
− | <li><p class="lead">Making affordable PDMS chips with a new manufacturing workflow.</p></li> | + | <li class="marginmod"><p class="lead">Making affordable PDMS chips with a new manufacturing workflow.</p></li> |
<a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/HardawareMicrofluidics"> | <a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/HardawareMicrofluidics"> | ||
<span class="btn__text"> | <span class="btn__text"> | ||
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</a> | </a> | ||
− | <li><p class="lead">Helping future teams to discover more and more aptamers through a new SELEX protocol based on 3D printed nitrocellulose columns.</p></li> | + | <li class="marginmod"><p class="lead">Helping future teams to discover more and more aptamers through a new SELEX protocol based on 3D printed nitrocellulose columns.</p></li> |
<a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/AptamerProtocols#dicoverymenu"> | <a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/AptamerProtocols#dicoverymenu"> | ||
<span class="btn__text"> | <span class="btn__text"> | ||
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</a> | </a> | ||
− | <li><p class="lead">Developing a new quantitative method for aptamer characterization, improving the earlier semi-quantitative ways.</p></li> | + | <li class="marginmod"><p class="lead">Developing a new quantitative method for aptamer characterization, improving the earlier semi-quantitative ways.</p></li> |
<a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/AptCharacterization"> | <a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/AptCharacterization"> | ||
<span class="btn__text"> | <span class="btn__text"> | ||
Line 54: | Line 58: | ||
</a> | </a> | ||
− | <li><p class="lead">Establishing a simple and cost-effective method to make electrochemical measurements of protein concentration with aptamers.</p></li> | + | <li class="marginmod"><p class="lead">Establishing a simple and cost-effective method to make electrochemical measurements of protein concentration with aptamers.</p></li> |
<a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/ElectrodeIntegration"> | <a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/ElectrodeIntegration"> | ||
<span class="btn__text"> | <span class="btn__text"> | ||
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</a> | </a> | ||
− | <li><p class="lead">Integrating all the technologies in our final design, which is an automated device for aptasensing, easily replicable as long as it is made with digital fabrication techniques.</p></li> | + | <li class="marginmod"><p class="lead">Integrating all the technologies in our final design, which is an automated device for aptasensing, easily replicable as long as it is made with digital fabrication techniques.</p></li> |
<a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/FinalPrototype"> | <a class="btn btn--primary-2 btn--sm type--uppercase" href="https://2018.igem.org/Team:Madrid-OLM/FinalPrototype"> | ||
<span class="btn__text"> | <span class="btn__text"> |
Revision as of 18:23, 15 October 2018
Contribution
During this competition, we have faced a different kind of problems, but the principal ones were the lack of money and time. We have felt the absence of resources in both of the parts of our project, the aptamer development, of the device manufacturing. For solving this issue we have learned to make all the equipment that we had need during the project by ourselves, getting the inspiration in the DIYBio movement. Now we are proud to contribute to the iGEM community with a new successful way of:
Making affordable PDMS chips with a new manufacturing workflow.
Microfluidics
Helping future teams to discover more and more aptamers through a new SELEX protocol based on 3D printed nitrocellulose columns.
Selex Protocol
Developing a new quantitative method for aptamer characterization, improving the earlier semi-quantitative ways.
Aptamer Characterization
Establishing a simple and cost-effective method to make electrochemical measurements of protein concentration with aptamers.
Binding to electrode
Integrating all the technologies in our final design, which is an automated device for aptasensing, easily replicable as long as it is made with digital fabrication techniques.
Final Prototype