Difference between revisions of "Team:Calgary/Protocols"

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Revision as of 22:47, 15 October 2018

Team:Calgary/Notebook - 2018.igem.org

PROTOCOLS



Below are the protocols used by the team.

Materials
  • iGEM 2018 distribution kit
  • ddH₂O
    		•
    Protocol
    1. Add 10μL of ddH₂O to the desired well.
    2. Pipette up and down 3-5 times.
    3. Incubate at room temperature for 10 minutes.
    4. Transform cells with 1μL of rehydrated DNA as per transformation protocol. Store the remaining amount at -20°C.

    Materials
    • Synthesized DNA from IDT or Genscript
    • ddH₂O
    Protocol
    1. Centrifuge tube containing the synthesized DNA for 1 minute at 3000g.
    2. Add 20μL ddH₂O.
    3. Vortex for 1 minute.
    4. Incubate at 50°C for 15 minutes.
    5. Briefly centrifuge. Store at -20°C.

    Experimental Details

    Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
    Materials
    • iGEM 2017 distribution kit
    • ddH₂O
    Protocols
    1. Add 10μL of ddH₂O to the desired well.
    2. Pipette up and down 3-5 times.
    3. Incubate at room temperature for 10 minutes.
    4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

    Experimental Details

    Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
    Materials
    • iGEM 2017 distribution kit
    • ddH₂O
    Protocols
    1. Add 10μL of ddH₂O to the desired well.
    2. Pipette up and down 3-5 times.
    3. Incubate at room temperature for 10 minutes.
    4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

    Experimental Details

    Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
    Materials
    • iGEM 2017 distribution kit
    • ddH₂O
    Protocols
    1. Add 10μL of ddH₂O to the desired well.
    2. Pipette up and down 3-5 times.
    3. Incubate at room temperature for 10 minutes.
    4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

    Experimental Details

    Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
    Materials
    • iGEM 2017 distribution kit
    • ddH₂O
    Protocols
    1. Add 10μL of ddH₂O to the desired well.
    2. Pipette up and down 3-5 times.
    3. Incubate at room temperature for 10 minutes.
    4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.