Difference between revisions of "Team:Rheda Bielefeld/OverviewExperiment"

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After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
 
After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
 
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Our team also dealed with searching for DNA sequences saved in the database of NGBI.
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Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/" NCBI> </a>. How to use the program MEGA7 and the database is also mentioned in the Overview.
 
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Revision as of 14:07, 16 October 2018

Overview Experiments

For our project, we needed to do a lot of experiments. At first, it was a bit difficult to find some well working methods, but after a while of researching we were finally able to get very good results.

We did two assays [1] [2] to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.

After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR [1] [3] . Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.

Our team also dealed with searching for DNA sequences saved in the database of . How to use the program MEGA7 and the database is also mentioned in the Overview.

Pictures



Working at the assays