Difference between revisions of "Team:BJRS China/Design"

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<h1>Design</h1>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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<div class="navbar-header"><a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="120px" height="120px" style="position:relative;top:0px;left:90%;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BJRS_China--logo2.jpeg"width="1200px" height="50px"style="position:relative;top:-114px;left:10px;"></a></div>
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    <div>
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      <ul class="nav navbar-nav">
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a>
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          <ul class="dropdown-menu">
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Background">BACKGROUND</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Design">DESIGN</a></li> 
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Results">RESULTS</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Improve">IMPROVE</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/InterLab">INTERLAB</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Demonstrate">DEMONSTRATE</a></li>
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          </ul>
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        <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Human_Practices">Human Practices</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Engagement">Engagement</a></li>
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          </ul>
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        </li>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ACHIEVEMENTS <b class="caret"></b> </a>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Parts">PARTS</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Judging_Criteria">JUDGING CRITERIA</a></li>
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        <li><a href="https://2018.igem.org/Team:BJRS_China/Safety">SAFETY</a></li>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ATTRIBUTION <b class="caret"></b> </a>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Attributions">ATTRIBUTION</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Collaborations">COLLABORATION</a></li>
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          </ul>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <b class="caret"></b> </a>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Members">MEMBERS</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Notebooks">NOTEBOOKS</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Protocol">PROTOCOL</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Acknowledgement">ACKNOWLEDGEMENT</a></li>
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:BJRS_China/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
 
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          <h1 style = "text-align: center">Design</h1>
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<h3>What should this page contain?</h3>
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<ul>
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<li>Explanation of the engineering principles your team used in your design</li>
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<li>Discussion of the design iterations your team went through</li>
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<li>Experimental plan to test your designs</li>
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            <h2>Overview</h2>
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<p><font size="4">&emsp;&emsp;This year our project focused on enhancing the growth and production efficiency of the engineered bacteria during high-density fermentation. Previous research has found that expressing <i>Vitreoscilla</i> hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.<sup>[1]</sup> Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.<sup>[2]</sup> Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.</font></p>
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<h3>Inspiration</h3>
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
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Revision as of 18:04, 16 October 2018

BJRS


Design

Overview

  This year our project focused on enhancing the growth and production efficiency of the engineered bacteria during high-density fermentation. Previous research has found that expressing Vitreoscilla hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.[1] Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.[2] Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.