Difference between revisions of "Team:BJRS China/Design"
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<img src="https://static.igem.org/mediawiki/2018/8/85/T--BJRS_China--design2.jpg" width="60%" > | <img src="https://static.igem.org/mediawiki/2018/8/85/T--BJRS_China--design2.jpg" width="60%" > | ||
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| + | <h2>Surface display system</h2> | ||
| + | <p><font size="4">  Our project used three different surface display system including INP (<a href='http://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a>), intimin and autotransporter. We fused these surface transporter proteins with fluorescent protein meos and sfGFP respectively and observed the bacteria carrying these plasmids under confocal microscope to confirm that these systems could work effectively.</font></p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2018/a/a8/T--BJRS_China--design3.jpg" width="60%" > | ||
| + | <figcaption><div style="padding-left:300px;padding-right:300px"><b>Figure 1</b> The framework of the three kinds of surface displaying fluorescent protein parts. | ||
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Revision as of 18:17, 16 October 2018
Design
Overview
This year our project focused on enhancing the growth and production efficiency of the engineered bacteria during high-density fermentation. Previous research has found that expressing Vitreoscilla hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.[1] Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.[2] Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.
Surface display system
Our project used three different surface display system including INP (BBa_K523013), intimin and autotransporter. We fused these surface transporter proteins with fluorescent protein meos and sfGFP respectively and observed the bacteria carrying these plasmids under confocal microscope to confirm that these systems could work effectively.
