Difference between revisions of "Team:Pasteur Paris/Medals"
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| − | <td>We created a new BioBrick part as expected : <a href="http://parts.igem.org/Part:BBa_K2616000" style="font-weight: bold ; color:#85196a;"target="_blank">BBa_K2616000</a>. This part permits to secrete proNGF directly in the extracellular medium using E. coli type I secretion system. We used inducible promoter T7, in order to control proNGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. proNGF is adressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacid of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated proNGF from this 60 aminoacid long sequence by the cleaving site for TEV protease ENLYFQ. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. </td> | + | <td>We created a new BioBrick part as expected : <a href="http://parts.igem.org/Part:BBa_K2616000" style="font-weight: bold ; color:#85196a;"target="_blank">BBa_K2616000</a>. This part permits to secrete proNGF directly in the extracellular medium using <i>E. coli<i/> type I secretion system. We used inducible promoter T7, in order to control proNGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. proNGF is adressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacid of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated proNGF from this 60 aminoacid long sequence by the cleaving site for TEV protease ENLYFQ. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. </td> |
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Revision as of 22:21, 16 October 2018
Bronze medal criteria
| Explanation | Criteria achieved |
| Registration and Giant Jamboree Attendance | We registered for the iGEM Giant Jamboree and we can't wait to be there! |
| Competition Deliverables | We met the deadlines for every required deliverable. |
| Attributions | We completed the attributions page on our wiki. |
| Characterization / Contribution | We successfully completed the InterLab Measurement Study. |
Silver medal criteria
| Explanation | Criteria achieved |
| Validated Part / Validated Contribution | We created a new BioBrick part as expected : BBa_K2616000. This part permits to secrete proNGF directly in the extracellular medium using E. coli type I secretion system. We used inducible promoter T7, in order to control proNGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. proNGF is adressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacid of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated proNGF from this 60 aminoacid long sequence by the cleaving site for TEV protease ENLYFQ. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. |
| Collaboration | We hosted the 4th Parisian Meet-Up where we organized a Tiny Jamboree in the morning in order to practice with all the French teams for the Giant one in October. We also organized and attended round tables about bioethics with multiple professionals during the afternoon. We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this page . |
| Human Practices | We took into account the advice of many professionals in the conception and confinement of our interface NeuronArch. Our juridic team has worked with the Ethical Committee of the Pasteur Institute on the ethical and safety questions surrounding the use of GMOs inside the human body. We have tried to destigmatize the use of GMOs to the general public. Because we also thought about the consequences of a possible release of our GMOs in the environnement, we also integrated a thermosensitive Kill-Switch inside our bacteria. You can read about it on this page. |
Gold medal criteria
| Explanation | Criteria achieved |
| Integrated Human Practices | We thought with care of every aspect of our project by interviewing many experts of their fields (biofilms, infections, microfluidic and prosthesis) but also associations for the right of amputees as well as surgeons and took into account their advices in our final biological interface as well as our prototype. You can read about the progression of NeuronArch on this page |
| Improve a Previous Part or Project | We improved the part BBa_K237002 from the iGEM team SDU Denmark 2009. We optimized the RIP sequence for our chassis E. coli BL21 (DE3) pLysS strain and added a secretion signal peptide to address the RIP to E. coli Type II Secretion System. We confirmed our part BBa_K2616001 by sequencing and are still waiting for the result from the mass spectrometry platform. |
| Model Your Project | We modeled how NGF is produced in our modified E. Coli , its diffusion in a medium environment and its consequences on neurons growth. It helped to have an insight on which concentration of NGF to use in our experiments and also helped in predicting the future growth of nerves inside of our prototype. The mechanical modeling presents tools to visualize the constraints applied on the humerus and femur bone and was used to choose the best material to use for a prosthesis and the best configuration possible for a bone. Learn more about our modeling on this page |