CRISPR

Developing a cell line capable of simple, targeted, and large gene insertions

The Integration stage of our project was designed to develop a cell line which could facilitate the simple insertion of recombinant DNA into pre-made "socket" within the genome. This was attempted through two main steps: A CRISPR/Cas9 step and a recombinase-mediated step.

FLP-Beta

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Chromatin Modifying Elements

Maintenance of integrated genes via minimization of gene silencing and neighbourhood effects

Gene inserts are at risk of being rendered ineffective even after successful integration into the genome, as the spread of heterochromatin and DNA methylation can cause gene silencing. Furthermore, regulatory elements within both the insert and genome near the locus of integration may interact bidirectionally, leading to changes in gene expression known as neighbourhood effects. Chromatin modifying elements (CMEs) can help to generate an isolated, protected pocket within the genome, thereby assuring stable and sustained expression of integrated genes within eukaryotic systems.

Microfluidics

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Software

Each year, teams come to the iGEM competition with software that they have developed in conjunction with their research. However, we found it difficult to efficiently access these tools due to the sheer amount of projects and volume of wiki content. Thus, we created an online database called SARA, the Software Aggregating Research Assistant, which organizes software tools that have been created by iGEM teams and allows for the simplified searching. SARA also provides the opportunity for old software to be updated and improved to stay current, and decreases the likelihood that teams will create redundant software.