Difference between revisions of "Team:Rheda Bielefeld/OverviewExperiment"

Line 26: Line 26:
 
After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
 
After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
 
<br> <br>
 
<br> <br>
−
Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/" style="color:yellow;"> NCBI</a>. How to use the program MEGA7 and the database of NCBI is also mentioned in the Methods.
+
Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/" style="color:yellow;"> NCBI</a>. How to use the program MEGA7 and the database of NCBI is also mentioned in the Methods.
 
<br> <br>
 
<br> <br>
 
Our research also required a good handling with the light- and the electron microscope. We describe in the Methods how to use them.
 
Our research also required a good handling with the light- and the electron microscope. We describe in the Methods how to use them.

Revision as of 07:42, 17 October 2018

Home
The Team Our School Bielefeld University Rheda
Description Notebook
Overview Methods Pollen PCR Assays Cloning Strategy
Human Practices Marta Event
Safety Form Biosafety
Attributions Partners

Overview Experiments

For our project, we needed to do a lot of experiments. At first, it was a bit difficult to find some well working methods, but after a while of researching we were finally able to get very good results.

We did two assays [1] [2] to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.

After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR [1] [3] . Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.

Our team also dealed with searching for DNA sequences saved in the database of NCBI. How to use the program MEGA7 and the database of NCBI is also mentioned in the Methods.

Our research also required a good handling with the light- and the electron microscope. We describe in the Methods how to use them.

One essential part of our project is the cloning of [...]. If you want to see results and more about our cloning experiments, click here:

Pictures



Working at the assays