Difference between revisions of "Team:Calgary/Parts"
| Line 80: | Line 80: | ||
<p style="text-indent: 0px">Parts <b>BBa_K2605000 to BBa_K2605004 and BBa_K2605009</b> comprise the first iteration of a novel toolkit for targeted gene integration and maintenance of expression in eukaryotic chassis. The collection contains chromatin modifying elements that limit silencing of the integrated gene and minimize neighborhood effects. These elements, as well as the mCherry-BGH reporter gene with our improved CMV promoter, have restriction sites for directional cloning into part BBa_K2605002, which is a Multiple Cloning Site (MCS) flanked by distinct FRT (FLP recombinase recognition target) sites. The MCS carries sites for an arbitrary construct to be directionally cloned. </p> | <p style="text-indent: 0px">Parts <b>BBa_K2605000 to BBa_K2605004 and BBa_K2605009</b> comprise the first iteration of a novel toolkit for targeted gene integration and maintenance of expression in eukaryotic chassis. The collection contains chromatin modifying elements that limit silencing of the integrated gene and minimize neighborhood effects. These elements, as well as the mCherry-BGH reporter gene with our improved CMV promoter, have restriction sites for directional cloning into part BBa_K2605002, which is a Multiple Cloning Site (MCS) flanked by distinct FRT (FLP recombinase recognition target) sites. The MCS carries sites for an arbitrary construct to be directionally cloned. </p> | ||
<br> | <br> | ||
| − | + | <p style="text-indent: 0px">In the first iteration of our gene integration strategy, | |
the FRT sites are leveraged for recombinase-mediated casette exchange (RMCE). | the FRT sites are leveraged for recombinase-mediated casette exchange (RMCE). | ||
Representing an experimental second design,<b>BBa_K2605006</b> is a prototype part intended to test our novel FLP-Beta strategy. For either RMCE or FLP-Beta, the toolkit is intended to leverage genome-integrated recombinase target sites.</p> | Representing an experimental second design,<b>BBa_K2605006</b> is a prototype part intended to test our novel FLP-Beta strategy. For either RMCE or FLP-Beta, the toolkit is intended to leverage genome-integrated recombinase target sites.</p> | ||
Revision as of 09:02, 17 October 2018
PARTS
Basic Parts
BBa_K2605000- mCherry + BGH
Lorem ipsum dolor sit amet consectetur adipisicing elit. Consequuntur, totam laudantium, dolor porro laboriosam illo tenetur velit nulla corrupti quasi non eum amet quod dolores, doloremque eius ad temporibus perferendis! Lorem ipsum dolor sit, amet consectetur adipisicing elit. Sit explicabo suscipit similique id expedita cum consequatur voluptatibus consectetur adipisci beatae unde, cupiditate inventore. Quis officiis quam porro a expedita non.
BBa_K2605001- CMV with 5' SalI restriction site
Lorem ipsum dolor sit amet consectetur adipisicing elit. Temporibus rerum vel eius ut dolore, ab obcaecati officiis modi porro, sunt deleniti, consequatur assumenda asperiores aliquid recusandae tenetur neque quae suscipit! Lorem ipsum dolor sit amet consectetur adipisicing elit. Optio a quam iusto quo, nesciunt odit fuga, similique aspernatur veritatis nemo commodi libero nobis magnam necessitatibus, quidem maiores error debitis minima. Lorem ipsum dolor sit amet consectetur adipisicing elit. Quam ipsum consequatur, deserunt assumenda odio natus. Quis, ea dolor! Voluptas dolore, facere cum illo sunt consectetur nam a soluta optio perferendis.
BBa_K2605002- Multiple cloning site with flanking FRT sites
Lorem ipsum dolor sit, amet consectetur adipisicing elit. Rerum sit perferendis eum delectus odit vero saepe, dignissimos aspernatur et libero quisquam minima soluta a suscipit tempora dolores non aliquid ratione? Lorem ipsum dolor, sit amet consectetur adipisicing elit. Sunt excepturi quod, doloribus et asperiores similique tempora, mollitia possimus doloremque officia deleniti eius aut dolorum fuga reiciendis adipisci esse quisquam quae.
BBa_K2605003- A2UCOE
BBa_K2605004- Chicken beta-globin insulator
BBa_K2605005- FRT2
BBa_K2605006- Hybrid FlpO-beta resolvase
Composite Parts
BBa_K2605007- CMV with 5' SalI restriction site + monomeric RFP optimized for bacteria
BBa_K2605008- CMV + monomeric RFP optimized for bacteria
BBa_K2605009- CMV with 5' SalI restriction site + mCherry + BGH
BBa_K26050010- CMV + mCherry + BGH
Parts Collection
Parts BBa_K2605000 to BBa_K2605004 and BBa_K2605009 comprise the first iteration of a novel toolkit for targeted gene integration and maintenance of expression in eukaryotic chassis. The collection contains chromatin modifying elements that limit silencing of the integrated gene and minimize neighborhood effects. These elements, as well as the mCherry-BGH reporter gene with our improved CMV promoter, have restriction sites for directional cloning into part BBa_K2605002, which is a Multiple Cloning Site (MCS) flanked by distinct FRT (FLP recombinase recognition target) sites. The MCS carries sites for an arbitrary construct to be directionally cloned.
In the first iteration of our gene integration strategy, the FRT sites are leveraged for recombinase-mediated casette exchange (RMCE). Representing an experimental second design,BBa_K2605006 is a prototype part intended to test our novel FLP-Beta strategy. For either RMCE or FLP-Beta, the toolkit is intended to leverage genome-integrated recombinase target sites.