Difference between revisions of "Team:SCAU-China/Parts"
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Revision as of 09:27, 17 October 2018
The parts we used are listed at the table below.
| Part number | Type | Function description | Medal |
|---|---|---|---|
| BBa_K515107 | Composite | Dendra2 cording | Bronze |
| BBa_K2559000 | Basic | endoglucanase | Silver |
| BBa_K2559001 | Basic | cellulose complement protein A | Silver |
| BBa_K2559002 | Basic | β-glucosidase | Silver |
| BBa_K2559003 | Basic | E. coli. Promoter PrplJ | Unsubmit |
| BBa_K2559004 | Basic | E. coli. Promoter Pdapa | Unsubmit |
| BBa_K2559011 | Basic | E. coli. Promoter PcaiF | Unsubmit |
| BBa_K2559007 | Basic | Promoter of Heat Shock Protein Pgroel | Unsubmit |
| BBa_K2559008 | Basic | slr0168 | Unsubmit |
| BBa_K2559012 | Basic | Promoter of RuBisCO(Prbc) | Unsubmit |
| BBa_K2559013 | Basic | Terminator of RuBisCO(Prbc) | Unsubmit |
| BBa_K2559005 | Basic | enhance green fluorescent protein | Gold |
| BBa_K2559009 | Composite | expression of eGFP | Gold |
(1) BBa_K515107
We characterized the BBa_K515107 part comprising the coding sequence BBa_K515007 which coded for Dendra2, a photoconvertible reporter protein under the control of the repressible promoter TetR (BBa_R0040) and the RBS (BBa_B0034).
(2) BBa_K2559000/ BBa_K2559001/ BBa_K2559002
These three parts were obtained from the Acetobacter xylinum bacterial cellulose synthase, as they were suspected to function together to regulate the expression and secretion of bacterial cellulose.
The bcs Z(BBa_K2559000) is coding an endoglucanase in the process of cellulose hydrolysis, bcs H (BBa_K2559001) can express cellulose complement protein A which affects the expression levels of bcsB and bcsC, interacts with bcsD. bglX (BBa_K2559003) is a β-glucosidase.
(3) BBa_K2559003/ BBa_K2559004/ BBa_K2559011
The three parts BBa_K2559003, BBa_K2559004 and BBa_K2559011 were obtained from E. coli, and but all the promoter pRplj , pDapa and pCaif are totally different in expressional intensity(Figure1).
(4) BBa_K2559007/ BBa_K2559008/ BBa_K2559012/ BBa_K2559013
The four parts BBa_K2559007, BBa_K2559008 ,BBa_K2559012 BBa_K2559013 were used for the construction of our synergistic recombination and CRISPR/cas9 Kit
School's name:SCAU
Member's name:SCAU
Designed by:SCAU