Difference between revisions of "Team:SCAU-China/Improve"
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<html> | <html> | ||
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#arr-l,#arr-r { | #arr-l,#arr-r { | ||
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| − | + | width: 50px; | |
| − | + | height: 50px; | |
| − | + | background: url("https://static.igem.org/mediawiki/2018/b/b6/T--SCAU-China--toTop.png") no-repeat center center rgba(0, 0, 0, .3); | |
| − | + | position: fixed; | |
| − | + | right: 50px; | |
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| − | + | cursor: pointer; | |
| − | + | display: none; | |
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| − | + | position: relative; | |
} | } | ||
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| − | + | position: relative; | |
| − | + | width: 101%; | |
| − | + | margin: 0 auto; | |
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#title{ | #title{ | ||
| − | + | height: 100px; | |
| − | + | background: rgba(0, 0, 0, 0.3); | |
| − | + | text-align: left; | |
| − | + | font-size: 60px; | |
| − | + | color: white; | |
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height: 1710px; | height: 1710px; | ||
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#article1 p{ | #article1 p{ | ||
| − | + | text-align: left; | |
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| − | + | height: 50px; | |
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#art2 p{ | #art2 p{ | ||
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| − | + | ||
| − | + | font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; | |
| − | + | font-size:20px; | |
} | } | ||
| + | #art3 p{ | ||
| + | text-align: left; | ||
| + | position: relative; | ||
| + | color: white; | ||
| + | margin-top:0px; | ||
| + | |||
| + | font-family: "Helvetica Neue", Helvetica, Arial, sans-serif; | ||
| + | font-size:20px; | ||
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body { | body { | ||
| − | + | background-color: white; | |
} | } | ||
</style> | </style> | ||
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<span class="caret"></span> | <span class="caret"></span> | ||
<ul class='dropdown-menu'> | <ul class='dropdown-menu'> | ||
| − | + | <li><a href="https://2018.igem.org/Team:SCAU-China/ProjectOverview">Overview</a></li> | |
<li><a href="https://2018.igem.org/Team:SCAU-China/Background">Background</a></li> | <li><a href="https://2018.igem.org/Team:SCAU-China/Background">Background</a></li> | ||
<li><a href="https://2018.igem.org/Team:SCAU-China/Design">Design</a></li> | <li><a href="https://2018.igem.org/Team:SCAU-China/Design">Design</a></li> | ||
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<span class="caret"></span> | <span class="caret"></span> | ||
<ul class='dropdown-menu'> | <ul class='dropdown-menu'> | ||
| − | <li><a href="https://2018.igem.org/Team:SCAU-China/ | + | <li><a href="https://2018.igem.org/Team:SCAU-China/Model">Overview</a></li> |
| − | <li><a href="https://2018.igem.org/Team:SCAU-China/HAWNA">HAWNA</a></li> | + | <li><a href="https://2018.igem.org/Team:SCAU-China/Model/HAWNA">HAWNA</a></li> |
| − | <li><a href="https://2018.igem.org/Team:SCAU-China/PPIBoost">PPIBoost</a></li> | + | <li><a href="https://2018.igem.org/Team:SCAU-China/Model/PPIBoost">PPIBoost</a></li> |
| − | <li><a href="https://2018.igem.org/Team:SCAU-China/CultrueCondition">Cultrue Condition</a></li> | + | <li><a href="https://2018.igem.org/Team:SCAU-China/Model/CultrueCondition">Cultrue Condition</a></li> |
| − | + | </ul> | |
</li> | </li> | ||
</ul> | </ul> | ||
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</div> | </div> | ||
</div> | </div> | ||
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<div id="mainDiv"> | <div id="mainDiv"> | ||
<div class="heart"><!-- 版心 --> | <div class="heart"><!-- 版心 --> | ||
| − | + | <div id="mask"><!-- 半透明底板 --> | |
| − | + | ||
| − | + | <div class="DBoard" id="title"> | |
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| + | Improve | ||
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</div> | </div> | ||
| − | + | <div class="DBoard" id="title2"> | |
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| − | + | BBa_K2559005 /BBa_K2559009 | |
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| + | </div> | ||
| + | <div class="DBoard" id="art3"> | ||
| + | <p> | ||
| − | + | The part BBa_K2559006 has a sequence improvement on the basic part submitted by iGEM07_Peking (BBa_l714891) which encodes the SDY_eGFP. However, we found out a 16 bp nucleotides redundancy in the eGFP starting coding region in BBa_I714891, after checking the sequence of BBa_I714891 from NCBI. Therefore, we decided to delete the redundant 16 bp nucleotides in BBa_I714891 to amend the length of eGFP coding sequence. The amended eGFP coding biobrick is the BBa_K2559005. | |
| − | + | </p> | |
| − | + | <p>To test the function of BBa_K2559005, we designed a new E.coli expression vector containing our new part termed as BBa_K2559003under a strong E.coli endogenous promoter (PrplJ). Therefore, the amended eGFP in BBa_K2559005 was driven by PrplJ promoter, and expressed in DH10B. In addition, we also applied the BBa_K2559005 in the promoter intensity analysis of our other two new parts, the BBa_K2559004 and BBa_K2559011 which are relatively weaker E.coli endogenous promoters (PdapA and PcaiF) (Figure 1).</p> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/8/80/T--SCAU-China--Measurement014.png" alt="" /> | |
| − | + | <br><br> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--SCAU-China--Measurement015.png" alt="" /> | |
| − | + | <p style="font-size:16px; text-align: center;">Figure 1: Fluorescent intensity of amended eGFP driven by by PrplJ, PdapA, PcaiF promoter.</p> | |
| − | + | <p>We summarized that our improvedpart, the amended eGFP coding biobrick BBa_K2559005 worked well in DH10B. We also hoped that our improvement on the BBa_I714891 can help their future applications by other groups in the future. However, it is difficult for us to perform additional experiments with BBa_K2559005 and BBa_I714891 due to the unavailable BBa_I714891.</p> | |
| − | + | <p>To expand the application of BBa_K2559005, we searched theBBa_J04450stored in registry and do another improvement in the BBa_J04450. The BBa_J04450 is a strong RFP expression vector in E.coli. As the main page of BBa_J04450 mentioned, the E.coli colonies with BBa_J04450 were in red color under normal light after about 18 hour culture on LB plate (Figure 2). We used the BBa_K2559005 to replace the RFP region in BBa_J04450, the modified part is BBa_K2559009. We transferred the BBa_K2559009 to DH5α by heat-shock, and found that the fluorescence signal can be observed under the UV (Figure 2).</p> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/9/94/T--SCAU-CHINA--improve3.jpg" width="700px" height="700px" /> | |
| − | + | <p style="font-size:16px; text-align: center;">Figure 2 Colonies with BBa_J04450 (red colony) and BBa_K2559009 (green colony) visualized under UV lightbox.</p> | |
| − | + | <p>So, we confirm that our improved part BBa_K2559005 can work in different E.coli expression system. We are also looking forward to more application of the BBa_K2559009!</p> | |
| − | + | </div> | |
| − | + | ||
| + | <p>School's name:SCAU</p> | ||
| + | <p>Member's name:SCAU</p> | ||
| + | <p>Designed by:SCAU</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <!-- 回到顶部按钮 --> | ||
| + | <em id="toTop"></em> | ||
| − | + | <script> | |
| − | + | var dropdownBoxs = document.getElementsByClassName('dropdown'); | |
| − | + | var dropdownMenus = document.getElementsByClassName('dropdown-menu'); | |
| − | + | for (let i = 0 ; i < dropdownBoxs.length ; i ++) { | |
| + | // console.log(dropdownBoxs[i]); | ||
| + | // console.log(dropdownMenus[i]); | ||
| + | dropdownBoxs[i].index = i; | ||
| + | dropdownBoxs[i].onclick = function () { | ||
| + | var styles = document.defaultView.getComputedStyle(dropdownMenus[i]) || dropdownMenus[i].currentStyle; | ||
| + | // console.log(styles.display); | ||
| + | if (styles.display == 'none') { | ||
| + | for (let j = 0 ; j < dropdownBoxs.length ; j ++) { | ||
| + | dropdownMenus[j].style.display = 'none'; | ||
| + | dropdownMenus[i].style.display = 'block'; | ||
| + | } | ||
| + | } else { | ||
| + | for (let j = 0 ; j < dropdownBoxs.length ; j ++) { | ||
| + | dropdownMenus[j].style.display = 'none'; | ||
| + | } | ||
| + | } | ||
| + | } | ||
| + | } | ||
| + | </script> | ||
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| − | + | <script type="text/javascript"> | |
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| − | + | var a= function() { | |
| − | + | var box01 = document.getElementById("lists-DB"); | |
| − | + | var lis = document.getElementById("lists");//获得轮播图图片盒子 | |
| − | + | var btns = document.getElementById("buttons");//获得按钮盒子 | |
| − | + | var imgs = lis.getElementsByTagName("img");//获得图片伪数组 | |
| − | + | btns.style.width = 24 * (imgs.length - 2) + "px"; | |
| − | + | btns.style.marginLeft = -(12 * (imgs.length - 2)) + "px";//动态赋值 | |
| + | for(var i = 0;i < imgs.length - 2; i++){ | ||
| + | //动态生成小圆点 | ||
| + | var span = document.createElement("span"); | ||
| + | btns.appendChild(span); | ||
| + | } | ||
| − | |||
| − | |||
| − | + | //轮播图正式部分 | |
| − | + | var prev = document.getElementById("arr-l"); | |
| − | + | var next = document.getElementById("arr-r"); | |
| − | + | var animated = false;//防止计时器多次被触发变量 | |
| − | + | ||
| − | + | ||
| − | + | function animate(offset) { | |
| − | + | var time = 300;//滚动一张图片总用时 | |
| − | + | var inteval = 10;//滚动一次的间隔时间 | |
| − | + | var speed = offset / (time / inteval);//每次滚动滑动的像素 | |
| + | var left = parseInt(lis.style.left) + offset;//先计算出滚动后的left值 | ||
| + | function go() { | ||
| + | animated = true;//为true代表正在运行 | ||
| + | //滑动函数 | ||
| + | if(((speed > 0) + (parseInt(lis.style.left) < left)===2) || ((speed < 0) + (parseInt(lis.style.left)) > left===2)){ | ||
| + | lis.style.left = parseInt(lis.style.left) + speed + "px"; | ||
| + | setTimeout(go,inteval);//设置计时器 | ||
| + | } | ||
| + | else { | ||
| + | lis.style.left = left + "px"; | ||
| + | if(parseInt(lis.style.left) > -1000) lis.style.left = -(1000 * (imgs.length - 2)) + "px"; | ||
| + | if(parseInt(lis.style.left) < -(1000 * (imgs.length - 2))) lis.style.left = -1000 + "px"; | ||
| + | animated = false;//结束运行 | ||
| + | } | ||
| + | } | ||
| + | go();//调用函数 | ||
| + | |||
| + | } | ||
| + | prev.onclick = function() { | ||
| + | if(animated) return;//正在轮播停止 | ||
| + | if(index == 0) index = spans.length - 1; | ||
| + | else index --; | ||
| + | animate(1000); | ||
| + | showButton(); | ||
| + | } | ||
| + | next.onclick = function() { | ||
| + | if(animated) return; | ||
| + | if(index == spans.length - 1) index = 0; | ||
| + | else index ++; | ||
| + | animate(-1000); | ||
| + | showButton(); | ||
| + | } | ||
| + | var index = 0;//记录现在className为on的按钮 | ||
| + | |||
| + | var spans = btns.getElementsByTagName("span");//得到btns下所有的span标签 | ||
| + | spans[0].className = "on"; | ||
| + | for(var i = 0;i < spans.length;i ++ ) { | ||
| + | spans[i].index = i;//自定义属性 | ||
| + | spans[i].onclick = function() { | ||
| + | if(animated) return; | ||
| + | if(this.className == "on") return; | ||
| + | animate((index - this.index) * 1000); | ||
| + | index = this.index; | ||
| + | showButton(); | ||
| + | } | ||
| + | } | ||
| + | |||
| + | function showButton() { //显示按钮运动的函数 | ||
| + | for(var j = 0;j < spans.length;j ++) {//排它思想 | ||
| + | //if(spans[j].className == "on") oldIndex = j; | ||
| + | spans[j].className = ""; | ||
| + | spans[index].className = "on"; | ||
| + | } | ||
| + | } | ||
| + | |||
| + | var timer;//计时器变量 , 这里为什么不能为null | ||
| + | var interval = 3000;//点击间隔时间 | ||
| + | |||
| + | function play() {//自动点击next函数 | ||
| + | timer = setTimeout(function(){ | ||
| + | next.onclick();//自动点击next | ||
| + | play();//递归调用 | ||
| + | },interval); | ||
| + | } | ||
| + | |||
| + | function stop() { | ||
| + | //停止函数 | ||
| + | clearTimeout(timer); | ||
| + | } | ||
| − | + | box01.onmouseover = stop; //鼠标悬浮在轮播图上时停止 | |
| − | + | box01.onmouseout = play;//鼠标离开轮播图继续 | |
| − | + | play();//先自己调用一次 | |
} | } | ||
a(); | a(); | ||
| − | + | </script> | |
| − | + | <script type="text/javascript"> | |
| − | + | //封装函数js文件，方便调用 | |
| − | + | function $(id) {return document.getElementById(id);}//封装获取id对象函数 | |
| − | + | function show(obj) {obj.style.display = "block";}//封装显示函数 | |
| − | + | function hidden(obj) {obj.style.display = "none";}//封装隐藏函数 | |
| − | + | function scroll(){ | |
| − | + | if(window.pageYOffset != null) { | |
| − | + | //ie9+ 和 其他浏览器 | |
| − | + | return { | |
| − | + | top: window.pageYOffset, | |
| − | + | left: window.pageXOffset | |
| − | + | } | |
| − | + | } | |
| − | + | else if(document.compatMode == "CSS1Compat") { | |
| − | + | //非怪异浏览器：没有头部的 | |
| − | + | return { | |
| − | + | top: document.documentElement.scrollTop, | |
| − | + | left: document.documentElement.scrollLeft | |
| − | + | } | |
| − | + | } | |
| − | + | return {//剩下的全部都是怪异浏览器 | |
| − | + | top: document.body.scrollTop, | |
| − | + | left: document.body.scrollLeft | |
| − | + | } | |
| − | + | } | |
| − | + | var leader = 0,target = 0,timer = null;//计时器变量 | |
| − | + | window.onscroll = function() { | |
| − | + | //滚动时执行的函数 | |
| − | + | scroll().top > 0 ? show($("toTop")) : hidden($("toTop"));//隐藏和显示totop | |
| − | + | leader = scroll().top; //实时记录滚动距离 | |
| − | + | } | |
| − | + | $("toTop").onclick = function() { | |
| − | + | //toTop被点击时执行的函数 | |
| − | + | target = 0; | |
| − | + | timer = setInterval(function() { | |
| − | + | leader = leader + (target - leader) / 10; | |
| − | + | window.scrollTo(0,leader); | |
| − | + | if(leader == target) clearInterval(timer);//当到达顶端清除计时器 | |
| − | + | //必须写在里面,因为在计时器执行时判断 | |
| − | + | },20); | |
| − | + | } | |
| − | + | </script> | |
</html> | </html> | ||
Latest revision as of 11:16, 17 October 2018
Improve
BBa_K2559005 /BBa_K2559009
The part BBa_K2559006 has a sequence improvement on the basic part submitted by iGEM07_Peking (BBa_l714891) which encodes the SDY_eGFP. However, we found out a 16 bp nucleotides redundancy in the eGFP starting coding region in BBa_I714891, after checking the sequence of BBa_I714891 from NCBI. Therefore, we decided to delete the redundant 16 bp nucleotides in BBa_I714891 to amend the length of eGFP coding sequence. The amended eGFP coding biobrick is the BBa_K2559005.
To test the function of BBa_K2559005, we designed a new E.coli expression vector containing our new part termed as BBa_K2559003under a strong E.coli endogenous promoter (PrplJ). Therefore, the amended eGFP in BBa_K2559005 was driven by PrplJ promoter, and expressed in DH10B. In addition, we also applied the BBa_K2559005 in the promoter intensity analysis of our other two new parts, the BBa_K2559004 and BBa_K2559011 which are relatively weaker E.coli endogenous promoters (PdapA and PcaiF) (Figure 1).
Figure 1: Fluorescent intensity of amended eGFP driven by by PrplJ, PdapA, PcaiF promoter.
We summarized that our improvedpart, the amended eGFP coding biobrick BBa_K2559005 worked well in DH10B. We also hoped that our improvement on the BBa_I714891 can help their future applications by other groups in the future. However, it is difficult for us to perform additional experiments with BBa_K2559005 and BBa_I714891 due to the unavailable BBa_I714891.
To expand the application of BBa_K2559005, we searched theBBa_J04450stored in registry and do another improvement in the BBa_J04450. The BBa_J04450 is a strong RFP expression vector in E.coli. As the main page of BBa_J04450 mentioned, the E.coli colonies with BBa_J04450 were in red color under normal light after about 18 hour culture on LB plate (Figure 2). We used the BBa_K2559005 to replace the RFP region in BBa_J04450, the modified part is BBa_K2559009. We transferred the BBa_K2559009 to DH5α by heat-shock, and found that the fluorescence signal can be observed under the UV (Figure 2).
Figure 2 Colonies with BBa_J04450 (red colony) and BBa_K2559009 (green colony) visualized under UV lightbox.
So, we confirm that our improved part BBa_K2559005 can work in different E.coli expression system. We are also looking forward to more application of the BBa_K2559009!
School's name:SCAU
Member's name:SCAU
Designed by:SCAU