Difference between revisions of "Team:SCAU-China/Improve"

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{{SCAU-China}}
 
 
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<div class="column full_size judges-will-not-evaluate">
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a {
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<h3>★  ALERT! </h3>
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text-decoration: none;
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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}
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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</style>
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<div class="Tan MingYang"></div>
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<div class="Li JiaDong"></div>
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<div class="Huang XinLing"></div>
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<div class="Fan ZhongZhao"></div>
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 +
<div class="navbar-default">
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<div class="container">
 +
<div class="navbar-header">
 +
<a href="https://2018.igem.org/Team:SCAU-China" class="navbar-brand">SCAU-2018</a>
 
</div>
 
</div>
 +
<div class="navbar">
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<ul style="float: left;" class="nav">
 +
<li class="dropdown">
 +
<a href="javascript:void(0)">TEAM</a>
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<span class="caret"></span>
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<ul class='dropdown-menu'>
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<li>
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<a href="https://2018.igem.org/Team:SCAU-China/Members">Members</a>
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</li>
 +
<li>
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<a href="https://2018.igem.org/Team:SCAU-China/Attributions">Attributions</a>
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</li>
  
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</ul>
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</li>
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<li class="dropdown">
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<a href="javascript:void(0)">PROJECT</a>
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<span class="caret"></span>
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<ul class='dropdown-menu'>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/ProjectOverview">Overview</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Background">Background</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Design">Design</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/SRK">Synergistic Recombination Kit</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/MM">Mathematical Model of Biological Intrinsic Regulation System</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Type">Type II CRISPR/Cas 9 Kit</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/MOM">Method for Optimizing Microbial Cell Culture</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Outlook">Outlook</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Demonstrate">Demonstrate</a></li>
  
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<div class="clear"></div>
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</ul>
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</li>
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<li class="dropdown">
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<a href="javascript:void(0)">LAB WORK</a>
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<span class="caret"></span>
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<ul class='dropdown-menu'>
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<li><a href="https://2018.igem.org/Team:SCAU-China/Experiments">Experiments</a></li>
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<li><a href="https://2018.igem.org/Team:SCAU-China/Parts">Parts</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Improve">Improve</a></li>
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<li><a href="https://2018.igem.org/Team:SCAU-China/InterLab">Interlab</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Measurement">Measurement</a></li>
 +
</ul>
 +
</li>
  
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<li class="dropdown">
 +
<a href="javascript:void(0)">MODEL</a>
 +
<span class="caret"></span>
 +
<ul class='dropdown-menu'>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Model">Overview</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Model/HAWNA">HAWNA</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Model/PPIBoost">PPIBoost</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Model/CultrueCondition">Cultrue Condition</a></li>
 +
</ul>
 +
</li>
 +
</ul>
 +
<ul style="float: right;" class="nav">
 +
<li class="dropdown">
 +
<a href="javascript:void(0)">SAFETY</a>
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<span class="caret"></span>
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<ul class='dropdown-menu'>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Safety">Safety</a></li>
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</ul>
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</li>
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<li class="dropdown">
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<a href="javascript:void(0)">HUMAN PRACTICES</a>
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<span class="caret"></span>
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<ul class='dropdown-menu'>
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<li><a href="https://2018.igem.org/Team:SCAU-China/Human_Practices">Overview</a></li>
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<li><a href="https://2018.igem.org/Team:SCAU-China/silver">Silver</a></li>
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<li><a href="https://2018.igem.org/Team:SCAU-China/Integrated">Integrated</a></li>
 +
<li><a href="https://2018.igem.org/Team:SCAU-China/Public_Engagement">Public Engagement & Education</a></li>
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<li><a href="https://2018.igem.org/Team:SCAU-China/Collaborations">Collaborations</a></li>
  
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<div class="column full_size">
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</ul>
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<h1>Improve</h1>
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</li>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
+
</ul>
 +
</div>
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</div>
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</div>
  
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<h3>Gold Medal Criterion #2</h3>
 
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement.  Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
 
  
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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<div id="mainDiv">
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<div class="heart"><!-- 版心 -->
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<div id="mask"><!-- 半透明底板 -->
  
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<br><br>
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<div class="DBoard" id="title">  
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
+
  
 +
 +
Improve
  
 
</div>
 
</div>
 +
<div class="DBoard" id="title2">
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 +
BBa_K2559005 /BBa_K2559009
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</div>
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<div class="DBoard" id="art3">
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<p>
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 +
The part BBa_K2559006 has a sequence improvement on the basic part submitted by iGEM07_Peking (BBa_l714891) which encodes the SDY_eGFP. However, we found out a 16 bp nucleotides redundancy in the eGFP starting coding region in BBa_I714891, after checking the sequence of BBa_I714891 from NCBI. Therefore, we decided to delete the redundant 16 bp nucleotides in BBa_I714891 to amend the length of eGFP coding sequence. The amended eGFP coding biobrick is the BBa_K2559005.
 +
</p>
 +
<p>To test the function of BBa_K2559005, we designed a new E.coli expression vector containing our new part termed as BBa_K2559003under a strong E.coli endogenous promoter (PrplJ). Therefore, the amended eGFP in BBa_K2559005 was driven by PrplJ promoter, and expressed in DH10B. In addition, we also applied the BBa_K2559005 in the promoter intensity analysis of our other two new parts, the BBa_K2559004 and BBa_K2559011 which are relatively weaker E.coli endogenous promoters (PdapA and PcaiF) (Figure 1).</p>
 +
<img src="https://static.igem.org/mediawiki/2018/8/80/T--SCAU-China--Measurement014.png" alt="" />
 +
<br><br>
 +
<img src="https://static.igem.org/mediawiki/2018/3/3f/T--SCAU-China--Measurement015.png" alt="" />
 +
<p style="font-size:16px; text-align: center;">Figure 1: Fluorescent intensity of amended eGFP driven by by PrplJ, PdapA, PcaiF promoter.</p>
 +
<p>We summarized that our improvedpart, the amended eGFP coding biobrick BBa_K2559005 worked well in DH10B. We also hoped that our improvement on the BBa_I714891 can help their future applications by other groups in the future. However, it is difficult for us to perform additional experiments with BBa_K2559005 and BBa_I714891 due to the unavailable BBa_I714891.</p>
 +
<p>To expand the application of BBa_K2559005, we searched theBBa_J04450stored in registry and do another improvement in the BBa_J04450. The BBa_J04450 is a strong RFP expression vector in E.coli. As the main page of BBa_J04450 mentioned, the E.coli colonies with BBa_J04450 were in red color under normal light after about 18 hour culture on LB plate (Figure 2). We used the BBa_K2559005 to replace the RFP region in BBa_J04450, the modified part is BBa_K2559009. We transferred the BBa_K2559009 to DH5α by heat-shock, and found that the fluorescence signal can be observed under the UV (Figure 2).</p>
 +
<img src="https://static.igem.org/mediawiki/2018/9/94/T--SCAU-CHINA--improve3.jpg" width="700px" height="700px" />
 +
<p style="font-size:16px; text-align: center;">Figure 2 Colonies with BBa_J04450 (red colony) and BBa_K2559009 (green colony) visualized under UV lightbox.</p>
 +
<p>So, we confirm that our improved part BBa_K2559005 can work in different E.coli expression system. We are also looking forward to more application of the BBa_K2559009!</p>
 +
</div>
 +
 +
<p>School's name:SCAU</p>
 +
<p>Member's name:SCAU</p>
 +
<p>Designed by:SCAU</p>
 +
</div>
 +
</div>
 +
</div>
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<!-- 回到顶部按钮 -->
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for (let j = 0 ; j < dropdownBoxs.length ; j ++) {
 +
dropdownMenus[j].style.display = 'none';
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}
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}
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}
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}
 +
</script>
 +
 +
 +
<script type="text/javascript">
 +
 +
var a= function() {
 +
var box01 = document.getElementById("lists-DB");
 +
var lis = document.getElementById("lists");//获得轮播图图片盒子
 +
var btns = document.getElementById("buttons");//获得按钮盒子
 +
var imgs = lis.getElementsByTagName("img");//获得图片伪数组
 +
btns.style.width = 24 * (imgs.length - 2) + "px";
 +
btns.style.marginLeft = -(12 * (imgs.length - 2)) + "px";//动态赋值
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for(var i = 0;i < imgs.length - 2; i++){
 +
//动态生成小圆点
 +
var span = document.createElement("span");
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btns.appendChild(span);
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}
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//轮播图正式部分
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var prev = document.getElementById("arr-l");
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var next = document.getElementById("arr-r");
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var animated = false;//防止计时器多次被触发变量
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function animate(offset) {
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var time = 300;//滚动一张图片总用时
 +
var inteval = 10;//滚动一次的间隔时间
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var speed = offset / (time / inteval);//每次滚动滑动的像素
 +
var left = parseInt(lis.style.left) + offset;//先计算出滚动后的left值
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function go() {
 +
animated = true;//为true代表正在运行
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//滑动函数
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if(((speed > 0) + (parseInt(lis.style.left) < left)===2) || ((speed < 0) + (parseInt(lis.style.left)) > left===2)){
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lis.style.left = parseInt(lis.style.left) + speed + "px";
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setTimeout(go,inteval);//设置计时器
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}
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else {
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lis.style.left = left + "px";
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if(parseInt(lis.style.left) > -1000) lis.style.left = -(1000 * (imgs.length - 2)) + "px";
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if(parseInt(lis.style.left) < -(1000 * (imgs.length - 2))) lis.style.left = -1000 + "px";
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animated = false;//结束运行
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}
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}
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go();//调用函数
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}
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prev.onclick = function() {
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if(animated) return;//正在轮播停止
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if(index == 0) index = spans.length - 1;
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else index --;
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animate(1000);
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showButton();
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next.onclick = function() {
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if(animated) return;
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if(index == spans.length - 1) index = 0;
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else index ++;
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animate(-1000);
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showButton();
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}
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var index = 0;//记录现在className为on的按钮
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 +
var spans = btns.getElementsByTagName("span");//得到btns下所有的span标签
 +
spans[0].className = "on";
 +
for(var i = 0;i < spans.length;i ++ ) {
 +
spans[i].index = i;//自定义属性
 +
spans[i].onclick = function() {
 +
if(animated) return;
 +
if(this.className == "on") return;
 +
animate((index - this.index) * 1000);
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index = this.index;
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showButton();
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}
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}
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function showButton() { //显示按钮运动的函数
 +
for(var j = 0;j < spans.length;j ++) {//排它思想
 +
//if(spans[j].className == "on") oldIndex = j;
 +
spans[j].className = "";
 +
spans[index].className = "on";
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}
 +
}
 +
 +
var timer;//计时器变量 , 这里为什么不能为null
 +
var interval = 3000;//点击间隔时间
 +
 +
function play() {//自动点击next函数
 +
timer = setTimeout(function(){
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next.onclick();//自动点击next
 +
play();//递归调用
 +
},interval);
 +
}
 +
 +
function stop() {
 +
//停止函数
 +
clearTimeout(timer);
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}
 +
 +
 +
box01.onmouseover = stop; //鼠标悬浮在轮播图上时停止
 +
box01.onmouseout = play;//鼠标离开轮播图继续
 +
play();//先自己调用一次
 +
}
 +
a();
 +
</script>
 +
<script type="text/javascript">
 +
//封装函数js文件，方便调用
 +
function $(id) {return document.getElementById(id);}//封装获取id对象函数
 +
function show(obj) {obj.style.display = "block";}//封装显示函数
 +
function hidden(obj) {obj.style.display = "none";}//封装隐藏函数
 +
function scroll(){
 +
if(window.pageYOffset != null) {
 +
//ie9+ 和 其他浏览器
 +
return {
 +
top: window.pageYOffset,
 +
left: window.pageXOffset
 +
}
 +
}
 +
else if(document.compatMode == "CSS1Compat") {
 +
//非怪异浏览器：没有头部的
 +
return {
 +
top: document.documentElement.scrollTop,
 +
left: document.documentElement.scrollLeft
 +
}
 +
}
 +
return {//剩下的全部都是怪异浏览器
 +
top: document.body.scrollTop,
 +
left: document.body.scrollLeft
 +
}
 +
}
 +
 +
var leader = 0,target = 0,timer = null;//计时器变量
 +
window.onscroll = function() {
 +
//滚动时执行的函数
 +
scroll().top > 0 ? show($("toTop")) : hidden($("toTop"));//隐藏和显示totop
 +
leader = scroll().top; //实时记录滚动距离
 +
}
 +
 +
$("toTop").onclick = function() {
 +
//toTop被点击时执行的函数
 +
target = 0;
 +
timer = setInterval(function() {
 +
leader = leader + (target - leader) / 10;
 +
window.scrollTo(0,leader);
 +
if(leader == target) clearInterval(timer);//当到达顶端清除计时器
 +
//必须写在里面,因为在计时器执行时判断
 +
},20);
 +
}
  
 +
</script>
  
  
 
</html>
 
</html>

Latest revision as of 11:16, 17 October 2018

SCAU-2018
Improve
BBa_K2559005 /BBa_K2559009

The part BBa_K2559006 has a sequence improvement on the basic part submitted by iGEM07_Peking (BBa_l714891) which encodes the SDY_eGFP. However, we found out a 16 bp nucleotides redundancy in the eGFP starting coding region in BBa_I714891, after checking the sequence of BBa_I714891 from NCBI. Therefore, we decided to delete the redundant 16 bp nucleotides in BBa_I714891 to amend the length of eGFP coding sequence. The amended eGFP coding biobrick is the BBa_K2559005.

To test the function of BBa_K2559005, we designed a new E.coli expression vector containing our new part termed as BBa_K2559003under a strong E.coli endogenous promoter (PrplJ). Therefore, the amended eGFP in BBa_K2559005 was driven by PrplJ promoter, and expressed in DH10B. In addition, we also applied the BBa_K2559005 in the promoter intensity analysis of our other two new parts, the BBa_K2559004 and BBa_K2559011 which are relatively weaker E.coli endogenous promoters (PdapA and PcaiF) (Figure 1).



Figure 1: Fluorescent intensity of amended eGFP driven by by PrplJ, PdapA, PcaiF promoter.

We summarized that our improvedpart, the amended eGFP coding biobrick BBa_K2559005 worked well in DH10B. We also hoped that our improvement on the BBa_I714891 can help their future applications by other groups in the future. However, it is difficult for us to perform additional experiments with BBa_K2559005 and BBa_I714891 due to the unavailable BBa_I714891.

To expand the application of BBa_K2559005, we searched theBBa_J04450stored in registry and do another improvement in the BBa_J04450. The BBa_J04450 is a strong RFP expression vector in E.coli. As the main page of BBa_J04450 mentioned, the E.coli colonies with BBa_J04450 were in red color under normal light after about 18 hour culture on LB plate (Figure 2). We used the BBa_K2559005 to replace the RFP region in BBa_J04450, the modified part is BBa_K2559009. We transferred the BBa_K2559009 to DH5α by heat-shock, and found that the fluorescence signal can be observed under the UV (Figure 2).

Figure 2 Colonies with BBa_J04450 (red colony) and BBa_K2559009 (green colony) visualized under UV lightbox.

So, we confirm that our improved part BBa_K2559005 can work in different E.coli expression system. We are also looking forward to more application of the BBa_K2559009!

School's name:SCAU

Member's name:SCAU

Designed by:SCAU