-A: we collected pollen to work with
-B: we tried to integrate genes for pectinases into the E.coli so that it can produce pectinases to break the pollen
-C: we extracted the pollens’ DNA
-D+E: it is the idea of a test strip that identifies the pollen with our E.coli
Abstract
Although there is already useful medicine, we are convinced that we can optimize the use of such medicine and reduce the exposure to unnecessary drugs because they have negative side effects like lowering the personal performance capacity, becoming tired and many others.
Therefore we use a DNA-based method which uses pectinase and cellulase to open the pollen and isolates their DNA. This DNA will be used for a PCR with specific primers against the birch allergen "Bet". By hereby identifying pollen we aim to measure the current pollen exposure in the air.