Difference between revisions of "Team:BJRS China"

 
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  <div><a href="https://2018.igem.org"><img src="https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg" width="100px" height="100px" style="position:relative;top:15px;"></a></div>
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<div class="navbar-header"><a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="120px" height="120px" style="position:relative;top:0px;left:90%;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BJRS_China--logo2.jpeg"width="1200px" height="50px"style="position:relative;top:-114px;left:10px;"></a></div>
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<div class="navbar-header"><a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="100px" height="100px" style="position:relative;top:-15px;left:90%;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BJRS_China--logo2.jpeg"width="1200px" height="50px"style="position:relative;top:-108px;"></a>
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     <div>
 
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       <ul class="nav navbar-nav">
 
       <ul class="nav navbar-nav">
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a>
 
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             <li><a href="https://2018.igem.org/Team:BJRS_China">BACKGROUND</a></li>
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<li><a href="#">DESIGN</a></li>   
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Results">RESULTS</a></li>
 
             <li><a href="https://2018.igem.org/Team:BJRS_China/Improve">IMPROVE</a></li>
 
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<li><a href="https://2018.igem.org/Team:BJRS_China/InterLab">INTERLAB</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Demonstrate">DEMONSTRATE</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Measurement">MEASUREMENT</a></li>
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        <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a>
 
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             <li><a href="#">ENGAGEMENT</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Human_Practices">Human Practices</a></li>
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             <li><a href="#">GOLD INTERGRATED</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Public_Engagement">Public Engagement</a></li>
 
           </ul>
 
           </ul>
 
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ACHIEVEMENTS <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ACHIEVEMENTS <b class="caret"></b> </a>
 
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Applied_Design">JUDGING CRITERIA</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Judging_Criteria">JUDGING CRITERIA</a></li>
 
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         <li><a href="#">SAFETY</a></li>
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ATTRIBUTION <b class="caret"></b> </a>
 
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <b class="caret"></b> </a>
 
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             <li><a href="#">NOTEBOOKS</a></li>
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<li><a href="#">PROTOCOL</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Protocol">PROTOCOL</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Acknowledgement">ACKNOWLEDGEMENT</a></li>
 
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   <h1 class="text-center"><strong> OxygenMAX</strong></h1>
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   <h1 class="text-center"style="color:white"><strong> </strong></h1>
 
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  <p class="text-center"><font size="4">    Previous work have shown that the expression of bacterial Vitreoscilla hemoglobin (VHb) can help bacteria utilize oxygen more efficiently. However, the bacterial cell membrane makes efficient hemoglobin-oxygen contact a challenge. Based on this, our team designed a VHb surface display system to express VHb on the outermost shell of the bacteria to raise the hemoglobin-oxygen contacting efficiency. Consequently，this could help bacteria tolerate the low oxygen environment better. We named this system OxygenMAX system. Basically, our OxygenMAX system can be applied to industrial fermentation to raise the high-cell-density growth of the engineering bacteria in bioreactors. Also, allowing for the better growth ability of the OxygenMAX system carried bacteria, our system can help avoid contamination with miscellaneous bacteria in industrial fermentation. Moreover, our OxygenMAX system can be applied to other low-oxygen engineering bacteria working condition like biosensor in intestinal tract, water or soil.</font></p>
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  <p class="text-center"><font size="4">    With the rapid development of biological technology ，more and more engineering bacteria with multiple functions have been created, which can be applied to various fields such as industry production, agriculture, environment, diagnosis and therapy.</font></p>
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  <p class="text-center"><font size="4">    Industrial biotechnology, represented by the successive listing of polylactide, biosteel, and polylactic acid, has set off the third wave of biotechnology industry at the turn of the century, and has attracted more and more attention from the government and the public.</font></p>
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  <p class="text-center"><font size="4">    In January 2017, the National Development and Reform Commission issued the “The 13th five-year plan for biological industry development”, the planning is put forward to the biological manufacturing industry output value over one trillion yuan in 2020, bio-based products was 25%, the proportion in all chemical production and biological energy to replace fossil energy quantity more than 56 million tons of standard coal, in areas such as electricity, gas supply, heating, fuel to achieve the goal of full scale application.</font></p>
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  <p class="text-center"><font size="4">    However, in spite of the high attention, the commercialization of industrial biotechnology is not as fast as we expected，one of the important limiting factors is the low bioprocessing efficiency during microbial fermentation. At present, one way to solve this problem is to achieve the technology of high-cell-density growth.At present, one way to solve this problem is to develop that can be achieved. Among them, the key factor limiting cell density in bioreactors is the availability of oxygen during the late phases of fermentation.</font></p>
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  <p class="text-center"><font size="4">    The growth and metabolism of aerobic microorganisms both require oxygen. When the oxygen demand of bacteria is not met, the respiration of the bacteria is inhibited, thereby inhibiting growth, and even causing the accumulation of by-products. The cell metabolic rate will slow down, and the formation of metabolites will also be hindered.</font></p>
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  <p class="text-center"><font size="4">    For aerobic fermentation, dissolved oxygen is usually both a nutrient and an environmental factor. The activity of aerobic microorganism enzymes is highly dependent on oxygen, and the production of some products requires the participation of oxygen.</font></p>
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  <p class="text-center"><font size="4">    Compared with oxygen dissolved in pure water, the solubility of oxygen is significantly reduced in fermentation broth due to the presence of various dissolved nutrients, inorganic salts and metabolites of microorganisms. In addition, the concentration of dissolved oxygen varies with temperature, pressure and salt. In general, the higher the temperature, the more salt dissolved, the lower the dissolved oxygen in the water; The higher the pressure, the higher the dissolved oxygen in the water.</font></p>
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  <p class="text-center"><font size="4">    At present, the lack of oxygen in the fermentation process is mainly caused by the following two factors.</font></p>
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  <p class="text-center"><font size="4">    One is insufficient dissolved oxygen in the medium. Dissolved oxygen refers to the dissolved oxygen in the molecular state of water. Since oxygen is undissolved gas, under the condition of normal pressure, 25 ° C, oxygen solubility in the water in the air is only about 0.25 tendency for L. Therefore, if the outside world cannot supply oxygen in time during the fermentation process, the amount of dissolved oxygen in the water can only maintain a very short period of normal respiration of the bacteria, it will be exhausted. Therefore, it is necessary to constantly introduce sterile air into the fermentation system and further disperse it by stirring in the fermentation tank, so as to maintain a moderate concentration of dissolved oxygen in the fermentation liquid, but sometimes it is still unable to meet the oxygen demand of fermentation production. With the increase of fermentation tank volume, the raw material and power consumption of the fermentation process have increased to a large extent, and the demand for oxygen is also increasing.</font></p>
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Latest revision as of 14:18, 17 October 2018

BJRS