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<div id="text-container"> | <div id="text-container"> | ||
− | <p> | + | <p>It is impossible to use bacteriophages and to develop a detection device if we do not know what are the needs and the constraints in hospitals and in medical analysis laboratory. |
So we need to discuss our project with physicians, specialists and even with the whole population. We also developed partnerships with local research laboratories and companies, to borrow equipment, recover bacteria and bacteriophages, and, most important, ask them for advice. | So we need to discuss our project with physicians, specialists and even with the whole population. We also developed partnerships with local research laboratories and companies, to borrow equipment, recover bacteria and bacteriophages, and, most important, ask them for advice. | ||
</p> | </p> | ||
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<br> | <br> | ||
− | <p | + | <p> <b><a href="#GROUND">GROUND FLOOR:</a> </b> |
+ | We established partnerships with local companies like Biomérieux and local laboratories like TIMC in order to benefit from their advice/expertise and we had the opportunity to get some of their biological material. Our engineers and researcher partners gave us a lot of recommendations all along the summer and helped us to get an external point of view on our project.</p> | ||
<br> | <br> | ||
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<br> | <br> | ||
− | <p><b> <a href="#THIRD"> THIRD FLOOR:</a></b> All the societies must be implicated in our project. But it is essential for us to be aware of what people know about phage therapy and antimicrobial resistance. | + | <p><b> <a href="#THIRD"> THIRD FLOOR:</a></b> All the societies must be implicated in our project. But it is essential for us to be aware of what people know about phage therapy and antimicrobial resistance. It is important to evaluate people's knowledge and then produce an appropriate outreach. </p> |
<br> <br> | <br> <br> | ||
<center><h3>CLICK ON THE PEOPLE YOU WANT TO HEAR FROM ! Everybody has something interesting to share with you.</h3> </center> | <center><h3>CLICK ON THE PEOPLE YOU WANT TO HEAR FROM ! Everybody has something interesting to share with you.</h3> </center> | ||
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<div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | <div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | ||
<br><h3><center><font style="color:#666666;">Learn how partnerships had influenced our project !</font><center></h3> | <br><h3><center><font style="color:#666666;">Learn how partnerships had influenced our project !</font><center></h3> | ||
− | + | <br> | |
<div class="collapse slide"> | <div class="collapse slide"> | ||
<h2><font size="6"><center><font color="#4256f4"><FONT id="optitl">BIOMERIEUX</font></font></center></font></h2> | <h2><font size="6"><center><font color="#4256f4"><FONT id="optitl">BIOMERIEUX</font></font></center></font></h2> | ||
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</div> | </div> | ||
− | < | + | <p><br><br><br><br> <br><br></p> |
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<br> | <br> | ||
<center><FONT id="optitl"><font size="10"><font color="white">FIRST FLOOR:</font></font></font></center> | <center><FONT id="optitl"><font size="10"><font color="white">FIRST FLOOR:</font></font></font></center> | ||
− | <br></div><br><br> | + | <br></div><p><br><br></p> |
<div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | <div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | ||
<br> | <br> | ||
− | <h3 style="line-height:1.3"><font size="6"><center><font color="#4256f4"><FONT id="optitl">INTERVIEW WITH PIERRE-ALAIN FALCONNET<br>Medical Analysis Laboratory Manager<br>ORIADE NOVIALE | + | <h3 style="line-height:1.3"><font size="6"><center><font color="#4256f4"><FONT id="optitl">INTERVIEW WITH PIERRE-ALAIN FALCONNET<br>Medical Analysis Laboratory Manager<br>ORIADE NOVIALE</font></font></center></font></h3> |
<br> | <br> | ||
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<p>Yes | <p>Yes | ||
</p> | </p> | ||
+ | |||
+ | </div> | ||
+ | <div class="collapse slide"> | ||
+ | <h2><font size="5">Visit the medical analysis laboratory!</font></h2> | ||
+ | |||
+ | <br> | ||
+ | <figure><center><img src="https://static.igem.org/mediawiki/2018/d/da/T--Grenoble-Alpes--labo10.png" style="width:115vh"></center><br> | ||
+ | <figcaption><center>Figure 1: Immunoassay Section / Oriade Noviale Meylan </figcaption></figure></center> | ||
+ | <br> | ||
+ | <br> | ||
+ | <figure><center><img src="https://static.igem.org/mediawiki/2018/7/73/T--Grenoble-Alpes--labo11.png" style="width:115vh"></center><br> | ||
+ | <figcaption><center>Figure 2: Treadmill for petri dishes / Oriade Noviale Meylan | ||
+ | </figcaption></figure></center> | ||
+ | <br> | ||
+ | <br> | ||
+ | <figure><center><img src="https://static.igem.org/mediawiki/2018/3/35/T--Grenoble-Alpes--labo12.png" style="width:115vh"></center> | ||
+ | <br> | ||
+ | <figcaption><center>Figure 3: Pre-analysis Section / Oriade Noviale Meylan | ||
+ | </figcaption></figure></center> | ||
+ | <br> | ||
+ | <br> | ||
+ | <figure><center><img src="https://static.igem.org/mediawiki/2018/5/5e/T--Grenoble-Alpes--labo13.png" style="width:115vh"></center> | ||
+ | <br> | ||
+ | <figcaption><center>Figure 4: Bench of automatized microscope / Oriade Noviale Meylan | ||
+ | </figcaption></figure></center> | ||
+ | <br> | ||
+ | <br> | ||
+ | <figure><center><img src="https://static.igem.org/mediawiki/2018/e/ef/T--Grenoble-Alpes--labo14.png" style="width:115vh"></center> | ||
+ | <br> | ||
+ | <figcaption><center>Figure 5: Molecular Biology Section / Oriade Noviale Meylan | ||
+ | </figcaption></figure></center> | ||
+ | <br> | ||
+ | |||
</div> | </div> | ||
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− | < | + | <p><br><br><br><br> <br><br></p> |
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<br> | <br> | ||
</div> | </div> | ||
− | <br> | + | <p><br> |
− | <br> | + | <br></p> |
<div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | <div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | ||
<br> | <br> | ||
− | <h3 style="line-height:1.3"><font size="6"><center><font color="#4256f4"><FONT id="optitl">INTERVIEW WITH PR. MAX MAURIN<br>Head of Bacteriology Department<br>La Tronche Hospital | + | <h3 style="line-height:1.3"><font size="6"><center><font color="#4256f4"><FONT id="optitl">INTERVIEW WITH PR. MAX MAURIN<br>Head of Bacteriology Department<br>La Tronche Hospital</font></font></center></font></h3> |
<br> | <br> | ||
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<br> | <br> | ||
− | + | <p> | |
− | + | Dr. Maurin thinks that our system is very interesting and answers well the given problem. However, some biological risks have to be taken into account such as the dissemination of the system components. If these aspects are well evaluated and handled, the system is very promising. Yet, Dr. Morin thinks that it will be very difficult to play on the sensitivity and the specificity of our system to compete with today’s methods in molecular biology. Among all the different specificities of our system, it is mostly the bacteriophages selection that caught his attention the most.</p><p> | |
− | + | ||
− | + | ||
At last, Dr. Morin thinks that physicians do not have enough ethical formations and are not aware enough of new bioethical laws.</p> | At last, Dr. Morin thinks that physicians do not have enough ethical formations and are not aware enough of new bioethical laws.</p> | ||
<br> | <br> | ||
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“There are many strains that became resistant to most of the antibiotics” so “the therapeutic possibilities decreased little by little”.<br> | “There are many strains that became resistant to most of the antibiotics” so “the therapeutic possibilities decreased little by little”.<br> | ||
“It is a real issue for public health” but we know how to treat most of the cases though. | “It is a real issue for public health” but we know how to treat most of the cases though. | ||
− | “There is an emergence of of therapeutic dead-ends in the hospital, that we we did not see since long time ago. People have infections with zero treatment possible to cure them, | + | “There is an emergence of of therapeutic dead-ends in the hospital, that we we did not see since long time ago. People have infections with zero treatment possible to cure them, that is rare.” <br> |
“Each year, new resistances emerge and we see their prevalence increasing. That is a reality.” | “Each year, new resistances emerge and we see their prevalence increasing. That is a reality.” | ||
</p> | </p> | ||
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<div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | <div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | ||
<br> | <br> | ||
− | <h3 style="line-height:1.3"><font size="6"><center><font color="#4256f4"><FONT id="optitl">INTERVIEW WITH PR. OLIVIER EPAULARD<br>Head of the Infectious and Tropical Diseases Department<br>La Tronche Hospital | + | <h3 style="line-height:1.3"><font size="6"><center><font color="#4256f4"><FONT id="optitl">INTERVIEW WITH PR. OLIVIER EPAULARD<br>Head of the Infectious and Tropical Diseases Department<br>La Tronche Hospital</font></font></center></font></h3> |
<br> | <br> | ||
<figure><center><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Grenoble-Alpes--actors%26partOlivierEpaulard.jpeg" style="width:50vh"></center></figure> | <figure><center><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Grenoble-Alpes--actors%26partOlivierEpaulard.jpeg" style="width:50vh"></center></figure> | ||
<br> | <br> | ||
− | + | ||
<p>Pr. Epaulard thinks that the project is very interesting. He is not overly passionate about the identification aspect but rather by the therapy aspect of the project. The diagnostic tools he uses have already very good specificity and sensitivity. It is going to be hard to compete with them. He verified many aspects of the biology in the system and was pleased with our answers. </p> | <p>Pr. Epaulard thinks that the project is very interesting. He is not overly passionate about the identification aspect but rather by the therapy aspect of the project. The diagnostic tools he uses have already very good specificity and sensitivity. It is going to be hard to compete with them. He verified many aspects of the biology in the system and was pleased with our answers. </p> | ||
<p>Pr. Epaulard thinks that one can learn about bioethics through practice. Therefore, there is no need for more theoretical courses on ethics in a physician formation.</p> | <p>Pr. Epaulard thinks that one can learn about bioethics through practice. Therefore, there is no need for more theoretical courses on ethics in a physician formation.</p> | ||
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<figure><center><img src="https://static.igem.org/mediawiki/2018/8/8c/T--Grenoble-Alpes--actors%26partHG.png" style="width:50vh"></center></figure> | <figure><center><img src="https://static.igem.org/mediawiki/2018/8/8c/T--Grenoble-Alpes--actors%26partHG.png" style="width:50vh"></center></figure> | ||
<br> | <br> | ||
+ | <h2><center><font size="4"> We do not put the image and the name of H.G because phage therapy is not allowed in France</font></font><center></h2> | ||
<p>After getting infected feet wounds, H.G was treated with antibiotics but the infection was not cured. No antibiotic worked and the infection spread. The only way to treat this patient was to amputate the two legs. H.G had heard about phage therapy and contacted a doctor specialized on the subject. He chose to try phage therapy to cure his wounds. Bacteriophages were applied for a year on the wounds and he is now almost cured.</p><p> | <p>After getting infected feet wounds, H.G was treated with antibiotics but the infection was not cured. No antibiotic worked and the infection spread. The only way to treat this patient was to amputate the two legs. H.G had heard about phage therapy and contacted a doctor specialized on the subject. He chose to try phage therapy to cure his wounds. Bacteriophages were applied for a year on the wounds and he is now almost cured.</p><p> | ||
On the pictures, you can see the state of his left foot before and after the phage therapy. </p> | On the pictures, you can see the state of his left foot before and after the phage therapy. </p> | ||
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<h2><font size="5">Read the interview with H.G</font></h2> | <h2><font size="5">Read the interview with H.G</font></h2> | ||
<p><b><i>Where did the infection come from?</i></b></p> | <p><b><i>Where did the infection come from?</i></b></p> | ||
− | <p>A wound, I have hammer legs (claw feet/toes) (deformation of the toe), the toes do not touch the ground and in the shoe it made a light bulb. One evening a piece left and it made a sore and the infection came. It | + | <p>A wound, I have hammer legs (claw feet/toes) (deformation of the toe), the toes do not touch the ground and in the shoe it made a light bulb. One evening a piece left and it made a sore and the infection came. It has been 13 years since I have the infection. 6 months ago it was two feet.</p> |
<p><b><i>Were you treated early on right foot?</i></b></p> | <p><b><i>Were you treated early on right foot?</i></b></p> | ||
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<p><b><i>When did the antibiotics stop working?</i></b></p> | <p><b><i>When did the antibiotics stop working?</i></b></p> | ||
− | <p>It | + | <p>It has been 3 years since antibiotics are no longer effective.</p> |
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</p> | </p> | ||
− | <p><b><i>Yes it | + | <p><b><i>Yes it is true that you prefer to try rather than lose your leg</i></b></p> |
<p>Yes. There is a blood test today and then 4 days later then 8 days later and there are 8 sessions to be done. | <p>Yes. There is a blood test today and then 4 days later then 8 days later and there are 8 sessions to be done. | ||
</p> | </p> | ||
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</p> | </p> | ||
− | <p><b><i>Do you know if it | + | <p><b><i>Do you know if it is a phage cocktail or a phage in particular? |
</i></b></p> | </i></b></p> | ||
<p>One for <i>Staphylococcus</i> and one for the other bacterium. | <p>One for <i>Staphylococcus</i> and one for the other bacterium. | ||
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<p><b><i>How much does a treatment cost? | <p><b><i>How much does a treatment cost? | ||
</i></b></p> | </i></b></p> | ||
− | <p>It costs nothing, it | + | <p>It costs nothing, it is flasks, it costs 45 euros with all inclusive for 6 bottles! |
</p> | </p> | ||
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<p><b><i>Did the treatment hurt you? | <p><b><i>Did the treatment hurt you? | ||
</i></b></p> | </i></b></p> | ||
− | <p>No, we feel nothing at all, it | + | <p>No, we feel nothing at all, it is just put on wounds and swallow (morning fasting) and also I pay attention to what I eat, wholemeal bread, whole rice no potato no red meat, no coffee, no sugar .. it is for healing especially |
</p> | </p> | ||
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<center><FONT id="optitl"><font size="10"><font color="white">THIRD FLOOR: </font></font></font></center></div> | <center><FONT id="optitl"><font size="10"><font color="white">THIRD FLOOR: </font></font></font></center></div> | ||
− | <br><br> | + | <p><br><br></p> |
<div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | <div style="padding:3px; padding-left:6px; border:1px dotted #d0d0d0; border-left:5px solid #4256f4; margin-left:20px; background-color: #f2efef;"> | ||
− | <br><h3><center><font style="color:#666666;"> | + | <br><h3><center><font style="color:#666666;">Our Survey about antimicrobial resistance, phage therapy and iGEM</font><center></h3><br> |
<p>"Science without conscience is only ruin of the soul" said F. Rabelais. Therefore, what consciousness are we talking about ? That of the scientist alone, that of his peers, or even the collective consciousness of a society, of the whole of humanity? For a long time, science thought itself capable of inventing, evaluating and understanding the stakes of its discoveries by its protagonists alone, but it is clear that this vision no longer has any relevance today. We have entered a new era, that of post-modernity; it is characterized by a questioning of science | <p>"Science without conscience is only ruin of the soul" said F. Rabelais. Therefore, what consciousness are we talking about ? That of the scientist alone, that of his peers, or even the collective consciousness of a society, of the whole of humanity? For a long time, science thought itself capable of inventing, evaluating and understanding the stakes of its discoveries by its protagonists alone, but it is clear that this vision no longer has any relevance today. We have entered a new era, that of post-modernity; it is characterized by a questioning of science | ||
and its basic axioms, but also, a doubt as to the ability of its actors to consider all the | and its basic axioms, but also, a doubt as to the ability of its actors to consider all the | ||
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are at the heart of our lives and our future. Thus, they must be thought of and questioned, that | are at the heart of our lives and our future. Thus, they must be thought of and questioned, that | ||
in view of their potential consequences. The technique, but also the science that gives it body | in view of their potential consequences. The technique, but also the science that gives it body | ||
− | must be the concern of all | + | must be the concern of all. </p> |
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<p><i>Click <a href="https://static.igem.org/mediawiki/2018/6/66/T--Grenoble-Alpes--questactor.pdf"><b>here</b></a> if you want to read the full text.</i><br> | <p><i>Click <a href="https://static.igem.org/mediawiki/2018/6/66/T--Grenoble-Alpes--questactor.pdf"><b>here</b></a> if you want to read the full text.</i><br> |
Latest revision as of 17:05, 17 October 2018
Template loop detected: Template:Grenoble-Alpes
ACTORS AND PARTNERSHIPS
It is impossible to use bacteriophages and to develop a detection device if we do not know what are the needs and the constraints in hospitals and in medical analysis laboratory. So we need to discuss our project with physicians, specialists and even with the whole population. We also developed partnerships with local research laboratories and companies, to borrow equipment, recover bacteria and bacteriophages, and, most important, ask them for advice.
GROUND FLOOR: We established partnerships with local companies like Biomérieux and local laboratories like TIMC in order to benefit from their advice/expertise and we had the opportunity to get some of their biological material. Our engineers and researcher partners gave us a lot of recommendations all along the summer and helped us to get an external point of view on our project.
FIRST FLOOR: We visited a medical analysis laboratory and we met the laboratory manager. We discussed with him about the detection system used in his laboratory and about the advantages and limits of our system, if it was used in this kind of laboratory.
SECOND FLOOR: We met with doctors and patients who talked with us about bacteriophages and antibiotic resistance. We discussed with them to know if our detection system could be used in hospitals and what modification we should make in order to improve it. You can have a look at them on the third floor (see our schematic below).
THIRD FLOOR: All the societies must be implicated in our project. But it is essential for us to be aware of what people know about phage therapy and antimicrobial resistance. It is important to evaluate people's knowledge and then produce an appropriate outreach.
CLICK ON THE PEOPLE YOU WANT TO HEAR FROM ! Everybody has something interesting to share with you.
Learn how partnerships had influenced our project !
INTERVIEW WITH PIERRE-ALAIN FALCONNET
Medical Analysis Laboratory Manager
ORIADE NOVIALE
Medical Analysis Laboratory Manager
ORIADE NOVIALE
![](https://static.igem.org/mediawiki/2018/e/e8/T--Grenoble-Alpes--falcact.jpg)
We presented our system to Pierre Alain Falconnet to see if it could work in the context of a medical analysis laboratory. The system seems totally adapted to the laboratory use. According to him, the operating principles are good. It is still necessary to make specificity and sensitivity tests and to know the processing time of a sample.
The most important is to have an accreditation (COFRAC, CEIVD) because without this, the analysis laboratories will probably not want to have the system.
If the use of phages is regulated by a standard P2 and not P3 or P4, he could even use the machine in his own laboratory.
He offered to help us by giving us test samples.
INTERVIEW WITH PR. MAX MAURIN
Head of Bacteriology Department
La Tronche Hospital
Head of Bacteriology Department
La Tronche Hospital
![](https://static.igem.org/mediawiki/2018/b/b8/T--Grenoble-Alpes--actors%26partMAxMaurin.jpeg)
Dr. Maurin thinks that our system is very interesting and answers well the given problem. However, some biological risks have to be taken into account such as the dissemination of the system components. If these aspects are well evaluated and handled, the system is very promising. Yet, Dr. Morin thinks that it will be very difficult to play on the sensitivity and the specificity of our system to compete with today’s methods in molecular biology. Among all the different specificities of our system, it is mostly the bacteriophages selection that caught his attention the most.
At last, Dr. Morin thinks that physicians do not have enough ethical formations and are not aware enough of new bioethical laws.
INTERVIEW WITH PR. OLIVIER EPAULARD
Head of the Infectious and Tropical Diseases Department
La Tronche Hospital
Head of the Infectious and Tropical Diseases Department
La Tronche Hospital
![](https://static.igem.org/mediawiki/2018/e/e9/T--Grenoble-Alpes--actors%26partOlivierEpaulard.jpeg)
Pr. Epaulard thinks that the project is very interesting. He is not overly passionate about the identification aspect but rather by the therapy aspect of the project. The diagnostic tools he uses have already very good specificity and sensitivity. It is going to be hard to compete with them. He verified many aspects of the biology in the system and was pleased with our answers.
Pr. Epaulard thinks that one can learn about bioethics through practice. Therefore, there is no need for more theoretical courses on ethics in a physician formation.
INTERVIEW WITH H.G, PATIENT TREATED WITH PHAGE THERAPY
![](https://static.igem.org/mediawiki/2018/8/8c/T--Grenoble-Alpes--actors%26partHG.png)
We do not put the image and the name of H.G because phage therapy is not allowed in France
After getting infected feet wounds, H.G was treated with antibiotics but the infection was not cured. No antibiotic worked and the infection spread. The only way to treat this patient was to amputate the two legs. H.G had heard about phage therapy and contacted a doctor specialized on the subject. He chose to try phage therapy to cure his wounds. Bacteriophages were applied for a year on the wounds and he is now almost cured.
On the pictures, you can see the state of his left foot before and after the phage therapy.
Our Survey about antimicrobial resistance, phage therapy and iGEM
"Science without conscience is only ruin of the soul" said F. Rabelais. Therefore, what consciousness are we talking about ? That of the scientist alone, that of his peers, or even the collective consciousness of a society, of the whole of humanity? For a long time, science thought itself capable of inventing, evaluating and understanding the stakes of its discoveries by its protagonists alone, but it is clear that this vision no longer has any relevance today. We have entered a new era, that of post-modernity; it is characterized by a questioning of science and its basic axioms, but also, a doubt as to the ability of its actors to consider all the dimensions of their discipline, as well as their legitimacy to choose in the name of the community. The stakes of science and technology are unprecedented, these two disciplines are at the heart of our lives and our future. Thus, they must be thought of and questioned, that in view of their potential consequences. The technique, but also the science that gives it body must be the concern of all.
Click here if you want to read the full text.
And see our results in our video !