(34 intermediate revisions by 4 users not shown) | |||
Line 5: | Line 5: | ||
<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
<LINK rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Grenoble-Alpes/CSS" /> | <LINK rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Grenoble-Alpes/CSS" /> | ||
+ | <link rel="stylesheet" href="https://use.fontawesome.com/releases/v5.3.1/css/all.css" integrity="sha384-mzrmE5qonljUremFsqc01SB46JvROS7bZs3IO2EmfFsd15uHvIt+Y8vEf7N7fWAU" crossorigin="anonymous"> | ||
<style> | <style> | ||
#welcome{ | #welcome{ | ||
Line 10: | Line 11: | ||
} | } | ||
#text-container { | #text-container { | ||
− | padding: | + | padding:5vh; |
box-sizing:border-box; | box-sizing:border-box; | ||
− | margin-top:calc(50vh - | + | margin-top:calc(50vh - 57px); |
+ | margin-left:32vh;} | ||
#overlay { | #overlay { | ||
z-index:0; | z-index:0; | ||
Line 32: | Line 34: | ||
font-size: 1.5em!important; | font-size: 1.5em!important; | ||
margin: 1vh;} | margin: 1vh;} | ||
+ | .cellule { | ||
+ | position:relative; | ||
+ | transition: 0.3s ease; | ||
+ | cursor: pointer; | ||
+ | height: 40px;} | ||
+ | .cellule:hover { | ||
+ | transform: scale(1.2, 1.2); | ||
+ | } | ||
+ | #cellule1 { | ||
+ | top: 191px; | ||
+ | left: 370px;} | ||
+ | #cellule2 { | ||
+ | top: 0px; | ||
+ | left: 361px;} | ||
+ | #cellule3 { | ||
+ | top: 346px; | ||
+ | left: -322px;} | ||
+ | #cellule4 { | ||
+ | top: 411px; | ||
+ | left: 128px;} | ||
+ | #cellule5 { | ||
+ | top: 412px; | ||
+ | left: 436px;} | ||
+ | #cellule6 { | ||
+ | top: 151px; | ||
+ | left: -406px;} | ||
</style> | </style> | ||
Line 43: | Line 71: | ||
<div id="sous-menu"> | <div id="sous-menu"> | ||
<ul> | <ul> | ||
− | + | ||
− | + | ||
− | + | ||
<li> | <li> | ||
− | <a href="https://2018.igem.org/Team:Grenoble-Alpes/ | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/Description" id="current-nav">PROJECT </a> |
<ul> | <ul> | ||
− | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/ | + | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/biology" id="current-menu">BIOLOGY</a><ul><li> |
− | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/bacteria_choice">BACTERIA CHOICE</a> | |
− | + | </li><li> | |
− | + | ||
− | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/selection">TARGET SELECTION</a> | |
− | + | </li><li> | |
− | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis">PHAGE LYSIS & DNA EXTRACTION</a> | |
− | + | </li><li> | |
− | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">PROBE CONSTRUCTION</a> | |
− | + | </li><li> | |
− | + | ||
− | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation">CONSERVATION</a> | |
− | </li> | + | </li></ul></li> |
− | + | ||
− | + | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/Hardware">ENGINEERING</a></li> | |
− | + | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/Demonstrate">DEMONSTRATE</a></li></ul> | |
− | + | ||
− | </li> | + | </li></ul> |
− | + | ||
− | <a href="https://2018.igem.org/Team:Grenoble-Alpes/ | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </li> | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
<div id="text-container"> | <div id="text-container"> | ||
− | <p>This year, iGEM Grenoble-Alpes worked on the creation of a fully automated system capable of detecting pathogenic bacteria. This diagram below represents all the work that was done prior to the system design. </p><p>First of all, we had to choose which pathogenic bacteria we wanted to detect. This choice had to be coherent with the problematic approached. Actually, antibiotic resistance is a growing major concern and Pseudomonas aeruginosa is one of the most problematic bacterium nowadays. | + | <br> |
− | </p><p>After the bacteria choice, the next question was: What do we want to detect? So a target selection work using bioinformatics was made. | + | <p>This year, iGEM Grenoble-Alpes worked on the creation of a fully automated system capable of detecting pathogenic bacteria. This diagram below represents all the work that was done prior to the system design. </p><p>First of all, we had to <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/hybridation">choose which pathogenic bacteria</a></b> we wanted to detect. This choice had to be coherent with the problematic approached. Actually, antibiotic resistance is a growing major concern and Pseudomonas aeruginosa is one of the most problematic bacterium nowadays. After choosing the bacteria we wanted to make our proof of concept on, we got familiar with how bacteriophages work as an alternative to antibiotics and how to use then to <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis">extract the DNA</a></b> of our bacteria of interest. |
− | </p><p>Finally, the last step was the probe design leading to the probe construction. All these steps will be described in more details later. | + | </p><p>After the bacteria choice, the next question was: What do we want to detect? So a <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/selection">target selection </a></b>work using bioinformatics was made. |
− | </p><p>In parallel to the detection probes, we thought about the component conservation in the system. To do so, conservation and freeze-dry tests were realized on the DNA, enzymes and the bacteria in the system. | + | </p><p>Finally, the last step was the probe design leading to the <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">probe construction</a></b>. All these steps will be described in more details later. |
+ | </p><p>In parallel to the detection probes, we thought about the <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation">component conservation</a></b> in the system. To do so, conservation and freeze-dry tests were realized on the DNA, enzymes and the bacteria in the system. | ||
</p><p>All these points are essential for the efficiency of the detection system. | </p><p>All these points are essential for the efficiency of the detection system. | ||
</p> | </p> | ||
− | <img src="https://static.igem.org/mediawiki/2018/ | + | <br> |
+ | <div style="height:693px; width:939px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/b/b7/T--Grenoble-Alpes--fondbio.png" style="position: absolute; | ||
+ | height: 737px; | ||
+ | width: 950px;margin-left:20px;"> | ||
+ | <a href="https://2018.igem.org/Team:Grenoble-Alpes/selection"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/8/8f/T--Grenoble-Alpes--tstitle.png" id="cellule1" class="cellule"></a> | ||
+ | <a href="https://2018.igem.org/Team:Grenoble-Alpes/bacteria_choice"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/6/62/T--Grenoble-Alpes--bctitle.png" id="cellule2" class="cellule"></a> | ||
+ | <a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/0d/T--Grenoble-Alpes--conservation.png" id="cellule3" class="cellule"></a> | ||
+ | <a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/e/e1/T--Grenoble-Alpes--dnaextr.png" id="cellule4" class="cellule"></a> | ||
+ | <a href="https://2018.igem.org/Team:Grenoble-Alpes/construction"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--Grenoble-Alpes--hybrid.png" id="cellule5" class="cellule"></a> | ||
+ | <a href="https://2018.igem.org/Team:Grenoble-Alpes/construction"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/a/a2/T--Grenoble-Alpes--probe.png" id="cellule6" class="cellule"></a></div> | ||
Line 104: | Line 127: | ||
</div> | </div> | ||
</body> | </body> | ||
+ | <footer> | ||
+ | <a href="mailto:Igem.grenoble.alpes@gmail.com">Igem.grenoble.alpes@gmail.com</a> | ||
+ | <div id="icons"> | ||
+ | <a href="https://www.facebook.com/igemfrancegrenoble/?ref=br_rs"><i class="fab fa-facebook fa-2x"></i></a> | ||
+ | <a href="https://twitter.com/igem_grenoble?lang=fr"><i class="fab fa-twitter fa-2x"></i></a> | ||
+ | <a href="https://www.linkedin.com/in/igem-grenoble-367974116/?originalSubdomain=fr"><i class="fab fa-linkedin fa-2x"></i></a> | ||
+ | <a href=""></a> | ||
+ | </div> | ||
+ | </footer> | ||
</html> | </html> |
Latest revision as of 17:11, 17 October 2018
Template loop detected: Template:Grenoble-Alpes
BIOLOGY
This year, iGEM Grenoble-Alpes worked on the creation of a fully automated system capable of detecting pathogenic bacteria. This diagram below represents all the work that was done prior to the system design.
First of all, we had to choose which pathogenic bacteria we wanted to detect. This choice had to be coherent with the problematic approached. Actually, antibiotic resistance is a growing major concern and Pseudomonas aeruginosa is one of the most problematic bacterium nowadays. After choosing the bacteria we wanted to make our proof of concept on, we got familiar with how bacteriophages work as an alternative to antibiotics and how to use then to extract the DNA of our bacteria of interest.
After the bacteria choice, the next question was: What do we want to detect? So a target selection work using bioinformatics was made.
Finally, the last step was the probe design leading to the probe construction. All these steps will be described in more details later.
In parallel to the detection probes, we thought about the component conservation in the system. To do so, conservation and freeze-dry tests were realized on the DNA, enzymes and the bacteria in the system.
All these points are essential for the efficiency of the detection system.