Difference between revisions of "Team:Grenoble-Alpes/biology"

 
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<div id="sous-menu">
 
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<a href="https://2018.igem.org/Team:Grenoble-Alpes">HOME</a>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/team">TEAM</a>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/Description" id="current-nav">PROJECT </a>
 
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/team#team-members">TEAM MEMBERS</a></li>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/biology" id="current-menu">BIOLOGY</a><ul><li>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/team#advisors">ADVISORS</a></li>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/bacteria_choice">BACTERIA CHOICE</a>
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                                                <a href="https://2018.igem.org/Team:Grenoble-Alpes/selection">TARGET SELECTION</a>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/project" id="current-menu">PROJECT </a>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis">PHAGE LYSIS & DNA EXTRACTION</a>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/biology">BIOLOGY</a></li>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">PROBE CONSTRUCTION</a>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/proof_of_concept">PROOF OF CONCEPT</a></li>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation">CONSERVATION</a>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/modelling">
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MODELLING
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/Demonstrate">DEMONSTRATE</a></li></ul>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/events">EVENTS</a></li>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/ressources">RESSOURCES</a>
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/notebook">NOTEBOOK</a>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/protocols">PROTOCOLS</a></li>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/componants">COMPONANTS</a></li>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/medal_criteria">MEDAL CRITERIA</a></li>
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<p>This year, iGEM Grenoble-Alpes worked on the creation of a fully automated system capable of detecting pathogenic bacteria. This diagram below represents all the work that was done prior to the system design. </p><p>First of all, we had to <a href="url">choose which pathogenic bacteria</a> we wanted to detect. This choice had to be coherent with the problematic approached. Actually, antibiotic resistance is a growing major concern and Pseudomonas aeruginosa is one of the most problematic bacterium nowadays.  
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<p>This year, iGEM Grenoble-Alpes worked on the creation of a fully automated system capable of detecting pathogenic bacteria. This diagram below represents all the work that was done prior to the system design. </p><p>First of all, we had to <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/hybridation">choose which pathogenic bacteria</a></b> we wanted to detect. This choice had to be coherent with the problematic approached. Actually, antibiotic resistance is a growing major concern and Pseudomonas aeruginosa is one of the most problematic bacterium nowadays. After choosing the bacteria we wanted to make our proof of concept on, we got familiar with how bacteriophages work as an alternative to antibiotics and how to use then to <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis">extract the DNA</a></b> of our bacteria of interest.
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</p><p>After the bacteria choice, the next question was: What do we want to detect? So a <a href="https://2018.igem.org/Team:Grenoble-Alpes/selection">target selection </a>work using bioinformatics was made.  
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</p><p>After the bacteria choice, the next question was: What do we want to detect? So a <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/selection">target selection </a></b>work using bioinformatics was made.  
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</p><p>Finally, the last step was the probe design leading to the <a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">probe construction</a>. All these steps will be described in more details later.  
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</p><p>Finally, the last step was the probe design leading to the <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">probe construction</a></b>. All these steps will be described in more details later.  
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</p><p>In parallel to the detection probes, we thought about the <a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation">component conservation</a> in the system. To do so, conservation and freeze-dry tests were realized on the DNA, enzymes and the bacteria in the system.
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</p><p>In parallel to the detection probes, we thought about the <b><a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation">component conservation</a></b> in the system. To do so, conservation and freeze-dry tests were realized on the DNA, enzymes and the bacteria in the system.
 
</p><p>All these points are essential for the efficiency of the detection system.
 
</p><p>All these points are essential for the efficiency of the detection system.
 
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis">
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">
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Latest revision as of 17:11, 17 October 2018

Template loop detected: Template:Grenoble-Alpes

BIOLOGY


This year, iGEM Grenoble-Alpes worked on the creation of a fully automated system capable of detecting pathogenic bacteria. This diagram below represents all the work that was done prior to the system design.

First of all, we had to choose which pathogenic bacteria we wanted to detect. This choice had to be coherent with the problematic approached. Actually, antibiotic resistance is a growing major concern and Pseudomonas aeruginosa is one of the most problematic bacterium nowadays. After choosing the bacteria we wanted to make our proof of concept on, we got familiar with how bacteriophages work as an alternative to antibiotics and how to use then to extract the DNA of our bacteria of interest.

After the bacteria choice, the next question was: What do we want to detect? So a target selection work using bioinformatics was made.

Finally, the last step was the probe design leading to the probe construction. All these steps will be described in more details later.

In parallel to the detection probes, we thought about the component conservation in the system. To do so, conservation and freeze-dry tests were realized on the DNA, enzymes and the bacteria in the system.

All these points are essential for the efficiency of the detection system.