Difference between revisions of "Team:Grenoble-Alpes/components"

 
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<style>
 
<style>
 
#welcome{
 
#welcome{
background-image: url("https://static.igem.org/mediawiki/2018/7/76/T--Grenoble-Alpes--fluoWelcomPic.jpeg");
+
background-image: url("https://static.igem.org/mediawiki/2018/f/fb/T--Grenoble-Alpes--componentsWelcomePic.jpeg");
 
}
 
}
 
#text-container {
 
#text-container {
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margin-top:calc(50vh - 39px);}
 
margin-top:calc(50vh - 39px);}
 
#overlay {
 
#overlay {
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opacitu:0.3;
 
z-index:0;
 
z-index:0;
 
height:calc(100vh - 21px);
 
height:calc(100vh - 21px);
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<section id="corpus">
 
<section id="corpus">
<h1>
+
<h1><font size="9">
Biology
+
BIOLOGY
</h1>
+
</font> </h1>
  
 
<h4>
 
<h4>
Total=$
+
Total=$455.51
 
</h4>
 
</h4>
 
<br>
 
<br>
  
 
<center><p style="display: inline-block;" class="test">
 
<center><p style="display: inline-block;" class="test">
<a  href="#lysis"><img style="margin-left: 25px; margin-right: 25px;" src="https://static.igem.org/mediawiki/2018/c/cc/T--Grenoble-Alpes--componentsFIg1.png" style="width:30vh"/></a>
+
<a  href="#lysis"><img style="margin-left: 25px; margin-right: 25px; width:30vh" src="https://static.igem.org/mediawiki/2018/c/cc/T--Grenoble-Alpes--componentsFIg1.png"/></a>
<a  href="#DNAb"><img style="margin-left: 25px; margin-right: 25px;" src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="DNA extraction" title="DNA extraction"/></a>
+
<br>
<a href="#probe"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Probe design and digestion" title="Probe design and digestion" /></a>
+
<a  href="#DNAb"><img style="margin-left: 25px; margin-right: 25px;width:30vh" src="https://static.igem.org/mediawiki/2018/f/fb/T--Grenoble-Alpes--componentsFIg2.png" title="DNA extraction"/></a>
<a href="#hybrid"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Hybridization" title="Hybridization"/></a>
+
<br>
<a href="#transfo"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Transformation" title="Transformation" ></a>
+
<a href="#probe"><img style="margin-left: 25px; margin-right: 25px;width:30vh"  src="https://static.igem.org/mediawiki/2018/8/8d/T--Grenoble-Alpes--componentsFIg3.png" alt="Probe design and digestion" title="Probe design and digestion" /></a>
 +
<br>
 +
<a href="#hybrid"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/6/60/T--Grenoble-Alpes--componentsFIg4.png" alt="Hybridization" title="Hybridization"/></a>
 +
<br>
 +
<a href="#transfo"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/2/28/T--Grenoble-Alpes--componentsFIg5.png" alt="Transformation" title="Transformation" ></a>
 +
<br>
 +
<a href="#Utilities"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/8/89/T--Grenoble-Alpes--ComponentsButtonUtilities.png" alt="Utilities" title="Utilities" ></a>
 
</p></center>
 
</p></center>
<h1>
+
<h1><font size="9">
Engineering
+
ENGINEERING
</h1>
+
</font></h1>
  
 
<h4>
 
<h4>
Total=$1281.84
+
Total=$1350.84
 
</h4>
 
</h4>
 +
<br>
 
<center><p style="text-align:center;" class="test">
 
<center><p style="text-align:center;" class="test">
<a href="#autom"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Automation" title="Automation"/></a>
+
<a href="#autom"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/0/04/T--Grenoble-Alpes--componentsFIg6.png" alt="Automation" title="Automation"/></a>
<a href="#struct"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Structure" title="Structure" /></a>
+
<br>
<a href="#DNAe"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="DNA extraction" title="DNA extraction" /></a>
+
<a href="#struct"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/c/c7/T--Grenoble-Alpes--componentsFIg7.png" alt="Structure" title="Structure" /></a>
<a href="#Temp"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Temperature control" title="Temperature control" /></a>
+
<br>
<a href="#Fluo"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Fluorescence unit" title="Fluorescence unit" /></a>
+
<a href="#DNAe"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/f/fb/T--Grenoble-Alpes--componentsFIg2.png" alt="DNA extraction" title="DNA extraction" /></a>
<a href="#elec"><img style="margin-left: 25px; margin-right: 25px;"  src="https://static.igem.org/mediawiki/2018/d/d7/T--Grenoble-Alpes--ComponentsSubImage.png" alt="Electronic components" title="Electronic components"/></a>
+
<br>
 +
<a href="#Temp"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/8/8f/T--Grenoble-Alpes--componentsFIg8.png" alt="Temperature control" title="Temperature control" /></a>
 +
<br>
 +
<a href="#Fluo"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/2/2f/T--Grenoble-Alpes--componentsFIg9.png" alt="Fluorescence unit" title="Fluorescence unit" /></a>
 +
<br>
 +
<a href="#elec"><img style="margin-left: 25px; margin-right: 25px; width:30vh"  src="https://static.igem.org/mediawiki/2018/9/99/T--Grenoble-Alpes--componentsFIg10.png" alt="Electronic components" title="Electronic components"/></a>
 
</p></center>
 
</p></center>
 
 
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+
<hr>
  
 
<center><table style="margin-bottom: 100px;">
 
<center><table style="margin-bottom: 100px;">
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</th>
 
</th>
 
<th>
 
<th>
Price in $
+
Price in $ per µL
 
</th>
 
</th>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
73.40
+
1.64
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
72.37
+
0.81
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
73.40
+
0.82
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
67.20
+
0.75
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
97.64
+
4.91
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
65.48
+
5.06
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
56.29
+
0.57
 
</td>
 
</td>
 
</tr>
 
</tr>
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<tr>
 
<tr>
 
<td class="consumable">
 
<td class="consumable">
Fsp
+
FspI
 
</td>
 
</td>
 
<td>
 
<td>
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</td>
 
</td>
 
<td class="amount">
 
<td class="amount">
69.27
+
1.55
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="totamount">
 
<td class="totamount">
XX
+
16.11
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="totamount">
 
<td class="totamount">
XX
+
82.00
 +
</td>
 +
</tr>
 +
 
 +
</table></center>
 +
 
 +
<center><table style="margin-bottom: 100px;">
 +
<caption id="Utilities">
 +
Utilities
 +
</caption>
 +
<tr>
 +
<th>
 +
Consumable
 +
</th>
 +
<th>
 +
Where can it be found to an affordable price?
 +
</th>
 +
<th>
 +
Price in $
 +
</th>
 +
</tr>
 +
 
 +
<tr>
 +
<td class="consumable">
 +
Cones (x16)
 +
</td>
 +
<td>
 +
Eppendorf
 +
</td>
 +
<td class="amount">
 +
0.56
 +
</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td class="consumable">
 +
Eppendorf tubes (0.5 and 1.5 mL) x58
 +
</td>
 +
<td>
 +
Eppendorf
 +
</td>
 +
<td class="amount">
 +
5.76
 +
</td>
 +
</tr>
 +
 
 +
 
 +
<tr>
 +
<td class="tot" colspan="2">
 +
Total
 +
</td>
 +
<td class="totamount">
 +
6.32
 
</td>
 
</td>
 
</tr>
 
</tr>
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<td class="amount">
 
<td class="amount">
 
42.41
 
42.41
 +
</td>
 +
</tr>
 +
 +
<tr>
 +
<td class="consumable">
 +
Raspberry Pi 7" Touch screen display
 +
</td>
 +
<td>
 +
Raspberry Pi
 +
</td>
 +
<td class="amount">
 +
69.00
 
</td>
 
</td>
 
</tr>
 
</tr>
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</td>
 
</td>
 
<td class="totamount">
 
<td class="totamount">
295.78
+
364.78
 
</td>
 
</td>
 
</tr>
 
</tr>
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</body>
 
</body>
<footer>
+
<footer style="font-size: 12px;">
 
<a href="mailto:Igem.grenoble.alpes@gmail.com">Igem.grenoble.alpes@gmail.com</a>
 
<a href="mailto:Igem.grenoble.alpes@gmail.com">Igem.grenoble.alpes@gmail.com</a>
 
<div id="icons">
 
<div id="icons">

Latest revision as of 17:46, 17 October 2018

Template loop detected: Template:Grenoble-Alpes

COMPONENTS

This page presents, on one hand, the cost of a single analysis in the biology part and on the other hand, the cost of the device in the engineer part. You may click on the field you are interested in, to access to the detailed amount per sector. The overall cost is given in $.

BIOLOGY

Total=$455.51




Probe design and digestion
Hybridization
Transformation
Utilities

ENGINEERING

Total=$1350.84


Automation
Structure
DNA extraction
Temperature control
Fluorescence unit
Electronic components


Bacterial lysis
Consumable Where can it be found to an affordable price? Price in $
Phages Laval university 300.00
Lysis buffer bioMerieux 0.23
Total 300.23
DNA extraction
Consumable Where can it be found to an affordable price? Price in $
easyMAG Magnetic Silica bioMerieux 0.03
easyMAG Buffer 1 bioMerieux 0.23
easyMAG Buffer 2 bioMerieux 0.31
easyMAG Buffer 3 bioMerieux 0.28
Total 0.85
Probe design and digestion
Consumable Where can it be found to an affordable price? Price in $ per µL
Nt.BspQI NEB 1.64
Nb.BsmI NEB 0.81
Nb.BssSI NEB 0.82
NgoMIV NEB 0.75
SphI-HF Promega 4.91
HaeIII Promega 5.06
RsaI Promega 0.57
FspI NEB 1.55
Total 16.11
Hybridization
Consumable Where can it be found to an affordable price? Price in $
DNA probes / 0.00 (given by iGEM)
DNA / 0.00 (product of phage lysis)
Total 0.00
Transformation
Consumable Where can it be found to an affordable price? Price in $
TOP10 Home-made 0.00
DH5alpha Home-made 0.00
SOC Broth or LB Broth NEB 82.00
Total 82.00
Utilities
Consumable Where can it be found to an affordable price? Price in $
Cones (x16) Eppendorf 0.56
Eppendorf tubes (0.5 and 1.5 mL) x58 Eppendorf 5.76
Total 6.32

Automation
Consumable Where can it be found to an affordable price? Price in $
Stepper motor Nema 17 (x2) eMotion Tech 32.30
Arduino Mega Arduino 40.37
Raspberry Pi 3B+ Raspberry Pi 42.41
Raspberry Pi 7" Touch screen display Raspberry Pi 69.00
Magnetic switch (x4) GoTronic 18.00
Neodymium magnet Supermagnete 1.40
Coupler (x2) eMotion Tech 11.30
Electronic pipette Second hand reseller 150.00
Total 364.78
Structure
Consumable Where can it be found to an affordable price? Price in $
Plexiglas Hardware shop 80.74
Delrin (for machining) Amazon 38.64 (given by bioMerieux)
PLA (for 3D printing) eMotion Tech 23.07 (given by Grenoble-INP Phelma)
Aluminum slot Hardware shop 51.95
Ball bearing (x3) RS-Online 22.49 (given by Grenoble-INP Phema)
Screw (M3 to M8) Hardware shop 57.67
Total 274.56
DNA extraction
Consumable Where can it be found to an affordable price? Price in $
Neodymium magnet Supermagnete 5.60
Actuator Actuonix 70.00 (given by bioMerieux)
Total 75.60
Temperature control
Consumable Where can it be found to an affordable price? Price in $
Cartridge heater (x4) Farnell 69.20
Peltier unit (x4) Farnell 344.68 (given by Phelma)
Thermal paste LDLC 17.30 (given by LDLC Grenoble)
Radiator + fan (x2) Amazon 34.60 (salvage)
Infrared sensor Digikey 27.30
Total 493.08
Fluorescence unit
Consumable Where can it be found to an affordable price? Price in $
Filter EdmundOptics 51.91
Smartphone magnifying lens Amazon 4.61
Picamera noIR v2 Raspberry Pi 25.00
565 nm LED (x8) Farnell 1.84
Total 83.36
Electronic components
Consumable Where can it be found to an affordable price? Price in $
Resistor (x19) RS-Online 11.02
DRV8825 pololu (x2) eMotion Tech 23.07
Chemical capacitor (x2) GoTronic 0.46
Polyester capacitor RS-Online 0.45
Bipolar transistor (x6) RS-Online 0.72
Transistor MOSFET (x3) RS-Online 1.61
Diode (x3) GoTronic 5.19
5V-3A power supply RS 4.61
12V supply Amazon 12.33
Total 59.46