Difference between revisions of "Team:Jilin China/Attributions"

 
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   <!--<section class="s0"></section>-->
 
   <!--<section class="s0"></section>-->
 
   <ul class="sidenav">
 
   <ul class="sidenav">
−
   <li><a href="#pragraph_1">Team Accomplishment</a></li>
+
   <li><a href="#paragraph_1">Support</a></li>
−
   <li><a href="#pragraph_2">Support</a></li>
+
  <li><a href="#paragraph_2">Team Accomp.</a></li>
−
   <li><a href="#pragraph_3">Pre Coaching</a></li>
+
   <li><a href="#paragraph_3">HP</a></li>
−
   <li><a href="#pragraph_5">Acknowledgements</a></li>
+
   <li><a href="#paragraph_4">Sponsor</a></li>
 +
   <li><a href="#paragraph_5">Other Works</a></li>
 
   </ul>
 
   </ul>
 
   <section class="s2">
 
   <section class="s2">
 
   <ul>
 
   <ul>
−
     <li class="pragraph_1" id="pragraph_1">
+
     <li class="paragraph_1 start" id="paragraph_1">
 +
    <div>
 +
<h2>Support</h2>
 +
<p>Our project could finish only with helps from others. We really appreciate their kindness. Without specific explanation, all the people referred are from School of Life Sciences, Jilin University.</p>
 +
 +
<h3>General Support</h3>
 +
<p>We really appreciate Jilin University, School of Life Sciences and National Engineering Laboratory for AIDS Vaccine for their support in the iGEM competition. </p>
 +
<p>We sincerely showing graititude to Office of Undergraduate Education and Office of Global Engagement for their enormous help. </p>
 +
<p>Thanks our instructors, <b>Ali Hou</b> and <b>Yang Zhan</b>, and advisor <b>Jin Liu</b> for giving us the constructive suggestions and meticulous guidance to our project. </p>
 +
<p>Thanks our Coordinator of International Affairs <b>Siyu Zhou</b> for correcting our English writting on Wiki.</p>
 +
<p>We're truly grateful for <b>Gatekeeper</b> of our Life Science Building, who is most respectable, amiable and kind.</p>
 +
 
 +
 +
<h3>Technique Support</h3>
 +
                <p>Ph.D candidate <b>Xiangzhi Zeng</b> taght us how to make construction using Golden Gate Assembly method.
 +
<p>Senior Engineer <b>Tiejun Gu</b> taught us how to use apparatus we need in experiment.</p>
 +
<p>Ph.D candidate <b>Hongyan Xia</b> from Translational Cancer Research lab taught us the use of the plate reader.</p>
 +
<p>Senior Engineer <b>Aijun Zhang</b> from Core Facilities for life science and Master <b>Lin Zhou</b> taught us the use of the multifunctional plate reader. </p>
 +
<p>Ph.D candidate <b>Chu Wang</b> guided us PCR technique.</p>
 +
<p>Ph.D candidate <b>Fan Wu</b> guided us ultrasonic wave cleaving cells.</p>
 +
 +
<h3>Material and Apparatus Support</h3>
 +
<p>Dr. <b>Xinghong Zhao</b> ordered reagents for us.</p>
 +
<p>Dr. <b>Yubin Ge</b> provided us a plate reader.</p>
 +
<p>Dr. <b>Haifeng Tang</b> from National Biological Experiment Teaching Demonstration Center, Jilin University, provided us a PCR instrument.</p>
 +
<p>Ph.D candidate <b>Hongyan Xia</b> provided us Taq enzyme, dNTP mixture and 10x buffer. </p>
 +
<p>Ph.D candidates <b>Liyan Song</b> and <b>Hongge Wang</b> provided us PBS Buffer, micropipettor and thermostatic shaker.</p>
 +
<p>Ph.D candidate <b>Rui Zhu</b> provided multiple help on experiment instruments.
 +
                <p>Master <b>JiaoJiao Nie</b> provided us thermostatic shaker.</p>
 +
<p><b>Wan Lab</b> provided us thermostatic shaker and steam sterilizer.</p>
 +
<p>Master <b>Jie Guo</b> provided us a tube of Trans5α Chemically Competent Cell.</p>
 +
                <p><b>Xiang Lab</b> provided us ice maker.</p>
 +
 +
<h3>Model Support</h3>
 +
<p>Ph.D candidate <b>Haotian Guo</b> from INSERM gave us valuable suggestion about our modeling.</p>
 +
<p>Associate Pro.<b>Zouyong Kui</b> and Associate Pro.<b>Chunlin Cao</b> provided guidness on curve fitting.</p>
 +
<p>Associate Pro.<b>Yonggui Fu</b> provided advice to our modeling.</p>
 +
 +
<h3>Wiki Support</h3>
 +
<p>All codes were finished by our teammates.</p>
 +
 
 +
    </div>
 +
    </li>
 +
    <li class="paragraph_2" id="paragraph_2">
 +
    <div>
 +
    <h2>Team Accomplishment</h2>
 +
<p>Thanks for every team member's ideas in brainstorm. We designed the project together. <b>Dr. Ali Hou</b>, <b>Dr. Yang Zhan</b> and <b>Ph.D candidate Jin Liu</b> provide constructive suggestions to our project. Our team was responsible for all aspects of running the team. Here are some details of attribution: </p>
 +
<h3>Sequence Design</h3>
 +
<p><b>Xianbo Chen</b> and <b>Zhijie Gu</b> designed the sequence of the CspA mRNA thermosensors, <b>Jiangjiao Mao</b> designed the sequence of the thermosensors based on the RNA thermometers,  <b>Jiangjiao Mao</b>and  <b>Fangqi Liu</b> designed the sequence of the thermosensors based on theRNase E and <b>Fangqi Liu</b> designed the sequence of the thermosensors based one the RNase III. </p>
 +
<h3>Goldengate Assembly</h3>
 +
<p>Done by <b>Jiangjiao Mao</b>, <b>Shuting Zheng</b>, <b>Haimeng Yu</b> and <b>Cenrong Wang</b>. </p>
 +
<h3>Fluorescence Measurement</h3>
 +
<p>Done by <b>Zihao Wang</b>, <b>Fangqi Liu</b>, <b>Xutong Liu</b>, <b>Zhijie Gu</b>, <b>Xianbo Chen</b> and <b>Yu Ma</b>. They finished the experiments day and night. </p>
 +
<h3>Improvement</h3>
 +
<p>Done by <b>Hetian Yuan</b>, <b>Ruochen Chai</b> and <b>Rongzhen Yu</b>.</p>
 +
<h3>InterLab Study</h3>
 +
<p><b>Jiangjiao Mao</b> performed the calibration measurement. <b>Zihao Wang</b>, <b>Ruochen Chai</b> and <b>Hetian Yuan</b> performed the cell measurement. <b>Fangqi Liu</b> and <b>Xutong Liu</b> performed the CFU counts. </p>
 +
<h3>Biobrick Construction</h3>
 +
<p><b>Hetian Yuan</b>, <b>Ruochen Chai</b> and <b>Rongzhen Yu</b> construct the standard BioBricks. </p>
 +
<h3>Human Practices</h3>
 +
<p><b>Hetian Yuan</b>, <b>Ruochen Chai</b> and <b>Rongzhen Yu</b> designed our human practice work and coordinated and planned various HP activities. Besides, all of the team member participated the activities and gained a lot. </p>
 +
<h3>Wiki</h3>
 +
<p>Done by <b>Rongzhen Yu</b>.</p>
 +
<h3>Art Design</h3>
 +
<p>Done by <b>Rongzhen Yu</b>.</p>
 +
    </div>
 +
    </li>
 +
    <li class="paragraph_3" id="paragraph_3">
 
     <div>
 
     <div>
−
      <h2>Synthetic Promoters Construction</h2>
+
    <h2>Human Practices</h2>
−
       <p>With the development of synthetic biology, scientists have set a goal to construct de novo biobricks, parts and systems, while realize the strandardization  of biological system. De novo genetic biobricks are of great value in metabolic engineering and systems biology, especially. Promoters represent critical elements that can participate in gene expression and regulation, which play an important role in artificial systems. However, promoters from different sources have sophisticated regulation system, low universality, unpredictable expression intensity and lack of standardization, their applications in synthetic biology have been impeded. Fortunately, de novo promoters construction and identification have provided a brand new method to address this issue.</p>
+
      <h3>Acadamic Winter School</h3>
−
       <p>A prokaryotic promoter is made up of -35 region, -10 region, spacers, UP element as conservative regions and other non-conserved regions. According to current researches, a promoter is also considered as core promoter with its downstream sequence including 5’Untranslated Region (5’UTR). Strictly, promoters and ribosomes binding sites (RBS) are regulatory elements of transcription and translation respectively. Because of the short sequence of RBS, we connect synthetic RBS library to the library of synthetic core promoters, then a novel library of promoters can be constructed.</p>
+
       <p>Thanks <b>Dr.Yan Chen</b>, <b>Dr.Ali Hou</b> and <b>Dr.Yang Zhan</b> for supporting us conduct the iGEM winter school.</p>
 +
  <p><b>Zihao Wang</b>, <b>Zihan Lu</b>, <b>Ming Han</b>, <b>Zhongqiao Gan</b>, <b>Huadong Xing</b>, <b>Shuai Wang</b>, <b>Jin Liu</b> and <b>Letian Bao</b> taught cell biology, molecular biology, microbiology, genetic engineering, document retrieval, mathematical modeling and web authoring.</p>
 +
      <h3>STEM</h3>
 +
      <p>Thanks for Mr Qu Baofeng, manager of STEM center in United Experimental School of AHSJU (Affiliated High School Jilin University) and Livon, for providing its STEM platform for us to teach students.</p>  
 +
       <p>Thanks for Ms Jingjing Yang, biology teacher in United Experimental School of  AHSJU and Livon, for providing us advice for the teaching in junior high school.</p>
 +
      <p>Ms Ma and Ms Wang in United Experimental School of  AHSJU and Livon provided us the chance to communicate with the primary school students in Changchun.</p>
 +
      <p>Mr Wang and Ms Ji in United Experimental School of AHSJU and Livon provides us the chance to give courses to the junior high school students.</p>
 +
      <p><b>We really appreciate all the teachers, students and parents who participated in our activities!!! We can’t finish our EPE subject without them！</b></p>
 +
      <h3>TEDxJLU</h3>
 +
      <p>Thanks to the TEDx club of Jilin University for offering us chances to held an TEDx event.</p>  
 
     </div>
 
     </div>
 
     </li>
 
     </li>
−
     <li class="pragraph_2" id="pragraph_2">
+
     <li class="paragraph_4" id="paragraph_4">
 
     <div>
 
     <div>
−
       <h2>Temperature-induced Promoter Library Construction</h2>
+
       <h2>Sponsor</h2>
−
       <p>Compared to widely used chemically induced promoters, physically induced promoters don’t require special inducer, reducing cost while avoiding super-sensitivity of inducer concentration. Besides, physically induced promoters have special characteristics and a vast application prospect, and among all kinds of  physical factors, temperature has become our main concern this year with its vast prospect in fermentation engineering, drug targeting and other fields. As a result, our project, with a view to construct and characterize synthetic regulated Temperature-Sensitive promoter libraries for 2018 iGEM competition.</p>
+
       <p style="display:none;"></p>
−
      <p>According to current researches, there are three different types of biomolecules responsive to temperature: DNA, RNA and proteins. Actually, RNA is playing a dominant role in temperature sensing especially in bacteria. 5’UTR of mRNA forms secondary structure, in order to block or expose SD （Shine-Dalgarno sequence，binding to ribosomes in prokaryote cells）sequence. Compared to DNA and proteins, it is easier to reconstruct temperature-sensing RNA.</p>
+
      <p>We really appreciate <b>JIAXING TAIMEI TECHNOLOGY</b> for sponsoring us.</p>
−
      <p>Our primary objective is to construct a temperature-sensitive promoter library supplying the iGEM registry with temperature-sensitive promoter parts collection. However, natural temperature-sensitive promoters, which are in a small number lacking in standardization, are regulated by many trans-acting factors, we try to design and construct de novo synthetic temperature-sensitive promoters with current researches.
+
  <p>Introduction of TAIMEI:</p>
−
</p>
+
  <p>As a leading technology solution provider in the field of life sciences, Taimei Technology is dedicated to propel biopharmaceutical industry development through state-of-the-art information technology, with focus in the field of clinical research and pharmacovigilance. </p>
 +
  <p>Taimei Technology provides the cloud platform for seamless clinical research collaboration between sponsors, sites, CROs, patients, regulatory agencies and third-party providers. On the foundation of our platform are 6 technology-enabled solutions encompassing data management, project management, electronic regulatory submission, central medical imaging, pharmacovigilance, and drug logistics. With biopharma industry drug development pain points in mind, Taimei Technology is dedicated to improving R&D quality and efficiency for accelerating innovation time-to-market while reducing costs. </p>
 
     </div>
 
     </div>
 
     </li>
 
     </li>
−
    <li class="pragraph_3" id="pragraph_3">
+
<li class="paragraph_5" id="paragraph_5">
 
     <div>
 
     <div>
−
       <h2>What are we doing?</h2>
+
       <h2>Other Works</h2>
−
       <p>We construct our promoter library with the free combination of core promoter and 5＇UTR, then the expression efficiency is easily to be changed with the replacement of different core promoters and sequence changes of 5’UTR, realizing the bi-level regulation of both transcription and translation. Four synthetic standard regulated libraries have been constructed:</p>
+
       <p style="display:none;"></p>
−
       <ul>
+
       <p>Gene sequencing was done by Comate Bioscience Co. Ltd.</p>
−
      <li>(1) Heat-induced RNA thermosensors library, directional reconstructions to secondary structures of RNA thermometers;</li>
+
      <p>Gene synthesis was done by Genscript Biotechnology Co. Ltd.</p>
−
      <li>(2) Heat-repressible RNA thermosensors library, directional reconstructions to anti-RNaseE cleavage site;</li>
+
      <p>The reagents we used in our lab was bought from NEB, Takara, Transgen, Tiangen, etc.</p>
−
      <li>(3) Cold-induced RNA thermosensors library, directional reconstructions to mRNA pseudoknot of CspA;</li>
+
−
      <li>(4) Cold-repressible RNA thermosensors library, directional reconstructions to RNase III binding site.</li>
+
−
      </ul>
+
 
     </div>
 
     </div>
 
     </li>
 
     </li>

Latest revision as of 22:20, 17 October 2018

ATTRIBUTIONS

Attributions

  • Support

    Our project could finish only with helps from others. We really appreciate their kindness. Without specific explanation, all the people referred are from School of Life Sciences, Jilin University.

    General Support

    We really appreciate Jilin University, School of Life Sciences and National Engineering Laboratory for AIDS Vaccine for their support in the iGEM competition.

    We sincerely showing graititude to Office of Undergraduate Education and Office of Global Engagement for their enormous help.

    Thanks our instructors, Ali Hou and Yang Zhan, and advisor Jin Liu for giving us the constructive suggestions and meticulous guidance to our project.

    Thanks our Coordinator of International Affairs Siyu Zhou for correcting our English writting on Wiki.

    We're truly grateful for Gatekeeper of our Life Science Building, who is most respectable, amiable and kind.

    Technique Support

    Ph.D candidate Xiangzhi Zeng taght us how to make construction using Golden Gate Assembly method.

    Senior Engineer Tiejun Gu taught us how to use apparatus we need in experiment.

    Ph.D candidate Hongyan Xia from Translational Cancer Research lab taught us the use of the plate reader.

    Senior Engineer Aijun Zhang from Core Facilities for life science and Master Lin Zhou taught us the use of the multifunctional plate reader.

    Ph.D candidate Chu Wang guided us PCR technique.

    Ph.D candidate Fan Wu guided us ultrasonic wave cleaving cells.

    Material and Apparatus Support

    Dr. Xinghong Zhao ordered reagents for us.

    Dr. Yubin Ge provided us a plate reader.

    Dr. Haifeng Tang from National Biological Experiment Teaching Demonstration Center, Jilin University, provided us a PCR instrument.

    Ph.D candidate Hongyan Xia provided us Taq enzyme, dNTP mixture and 10x buffer.

    Ph.D candidates Liyan Song and Hongge Wang provided us PBS Buffer, micropipettor and thermostatic shaker.

    Ph.D candidate Rui Zhu provided multiple help on experiment instruments.

    Master JiaoJiao Nie provided us thermostatic shaker.

    Wan Lab provided us thermostatic shaker and steam sterilizer.

    Master Jie Guo provided us a tube of Trans5α Chemically Competent Cell.

    Xiang Lab provided us ice maker.

    Model Support

    Ph.D candidate Haotian Guo from INSERM gave us valuable suggestion about our modeling.

    Associate Pro.Zouyong Kui and Associate Pro.Chunlin Cao provided guidness on curve fitting.

    Associate Pro.Yonggui Fu provided advice to our modeling.

    Wiki Support

    All codes were finished by our teammates.

  • Team Accomplishment

    Thanks for every team member's ideas in brainstorm. We designed the project together. Dr. Ali Hou, Dr. Yang Zhan and Ph.D candidate Jin Liu provide constructive suggestions to our project. Our team was responsible for all aspects of running the team. Here are some details of attribution:

    Sequence Design

    Xianbo Chen and Zhijie Gu designed the sequence of the CspA mRNA thermosensors, Jiangjiao Mao designed the sequence of the thermosensors based on the RNA thermometers, Jiangjiao Maoand Fangqi Liu designed the sequence of the thermosensors based on theRNase E and Fangqi Liu designed the sequence of the thermosensors based one the RNase III.

    Goldengate Assembly

    Done by Jiangjiao Mao, Shuting Zheng, Haimeng Yu and Cenrong Wang.

    Fluorescence Measurement

    Done by Zihao Wang, Fangqi Liu, Xutong Liu, Zhijie Gu, Xianbo Chen and Yu Ma. They finished the experiments day and night.

    Improvement

    Done by Hetian Yuan, Ruochen Chai and Rongzhen Yu.

    InterLab Study

    Jiangjiao Mao performed the calibration measurement. Zihao Wang, Ruochen Chai and Hetian Yuan performed the cell measurement. Fangqi Liu and Xutong Liu performed the CFU counts.

    Biobrick Construction

    Hetian Yuan, Ruochen Chai and Rongzhen Yu construct the standard BioBricks.

    Human Practices

    Hetian Yuan, Ruochen Chai and Rongzhen Yu designed our human practice work and coordinated and planned various HP activities. Besides, all of the team member participated the activities and gained a lot.

    Wiki

    Done by Rongzhen Yu.

    Art Design

    Done by Rongzhen Yu.

  • Human Practices

    Acadamic Winter School

    Thanks Dr.Yan Chen, Dr.Ali Hou and Dr.Yang Zhan for supporting us conduct the iGEM winter school.

    Zihao Wang, Zihan Lu, Ming Han, Zhongqiao Gan, Huadong Xing, Shuai Wang, Jin Liu and Letian Bao taught cell biology, molecular biology, microbiology, genetic engineering, document retrieval, mathematical modeling and web authoring.

    STEM

    Thanks for Mr Qu Baofeng, manager of STEM center in United Experimental School of AHSJU (Affiliated High School Jilin University) and Livon, for providing its STEM platform for us to teach students.

    Thanks for Ms Jingjing Yang, biology teacher in United Experimental School of AHSJU and Livon, for providing us advice for the teaching in junior high school.

    Ms Ma and Ms Wang in United Experimental School of AHSJU and Livon provided us the chance to communicate with the primary school students in Changchun.

    Mr Wang and Ms Ji in United Experimental School of AHSJU and Livon provides us the chance to give courses to the junior high school students.

    We really appreciate all the teachers, students and parents who participated in our activities!!! We can’t finish our EPE subject without them！

    TEDxJLU

    Thanks to the TEDx club of Jilin University for offering us chances to held an TEDx event.

  • Sponsor

    We really appreciate JIAXING TAIMEI TECHNOLOGY for sponsoring us.

    Introduction of TAIMEI:

    As a leading technology solution provider in the field of life sciences, Taimei Technology is dedicated to propel biopharmaceutical industry development through state-of-the-art information technology, with focus in the field of clinical research and pharmacovigilance.

    Taimei Technology provides the cloud platform for seamless clinical research collaboration between sponsors, sites, CROs, patients, regulatory agencies and third-party providers. On the foundation of our platform are 6 technology-enabled solutions encompassing data management, project management, electronic regulatory submission, central medical imaging, pharmacovigilance, and drug logistics. With biopharma industry drug development pain points in mind, Taimei Technology is dedicated to improving R&D quality and efficiency for accelerating innovation time-to-market while reducing costs.

  • Other Works

    Gene sequencing was done by Comate Bioscience Co. Ltd.

    Gene synthesis was done by Genscript Biotechnology Co. Ltd.

    The reagents we used in our lab was bought from NEB, Takara, Transgen, Tiangen, etc.