Difference between revisions of "Team:NEFU China/Composite Part"

 
(16 intermediate revisions by 6 users not shown)
Line 2: Line 2:
 
<head>
 
<head>
 
<meta charset="utf-8">
 
<meta charset="utf-8">
−
<title>Untitled Document</title>
 
−
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&amp;action=raw&amp;ctype=text/css"  rel="stylesheet" type="text/css">
 
−
<style>
 
  
 +
<title>Composite Parts</title>
 +
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 +
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-style-compositeparts&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 +
 +
<style>
 
#top_title, #sideMenu{
 
#top_title, #sideMenu{
 
     display: none;
 
     display: none;
Line 15: Line 17:
 
     margin-top: 0px;
 
     margin-top: 0px;
 
     margin-left: 0px;
 
     margin-left: 0px;
 +
}
 +
#foot-title h3 {
 +
    color:rgba(165,165,165,1.00);
 +
    line-height: 20px;
 +
    font-size: 18px;
 +
}
 +
#menu li ul li:hover ul{
 +
    background:rgba(0,0,0,0.75)!important;
 +
}
 +
li#mainlevel_01 a {
 +
    color: #FFE5B5!important;
 +
 +
}
 +
li#mainlevel_01 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_02 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_02 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_03 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_03 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_05 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_05 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_06 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_06 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_07 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_07 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_08 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_08 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
#menu li ul li ul li a:hover {
 +
    color: rgba(0,223,252,1);
 +
    border-top: dotted 1px rgba(255,255,255,0.91);
 +
    border-bottom: dotted 1px rgba(255,255,255,0.91);
 +
    background: rgba(0,223,252,.02);
 +
}
 +
#nav .mainlevel a {
 +
    color: black;
 +
    text-decoration:none;
 +
    line-height:32px;
 +
    display:block;
 +
    padding:0 5px;
 +
    font-size: 25px!important;
 +
font-family: 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif, 'Apple Color Emoji', 'Segoe UI Emoji', 'Segoe UI Symbol', 'Noto Color Emoji' !important;
 +
}
 +
.layer-bottom {
 +
z-index: -2;
 +
position: absolute;
 +
margin-top: 36px!important;
 +
}
 +
.basicpart-content {
 +
    padding-left: 150px;
 +
    padding-right: 150px;
 +
    padding-top: 40px;
 
}
 
}
  
 +
table.table {
 +
    background-color: transparent;
 +
}
 +
 +
.table th {
 +
    font-size: 22px;
 +
}
 +
 +
.table tbody {
 +
    background-color: transparent!important;
 +
}
 +
 +
.table td {
 +
    background-color: transparent;
 +
    font-size: 20px;
 +
    vertical-align: middle!important;
 +
    line-height: 27px;
 +
}
 +
 +
table.table {
 +
    background-color: rgb(0,0,0,0.6)!important;
 +
    color: white;
 +
}
 +
#background-reference {
 +
    padding-top: 50px;
 +
    padding-left: 150px;
 +
    padding-right:150px;
 +
    border:2px;
 +
    border-style: none;
 +
    font-family:"Open Sans" !important;
 +
    font-size:15px;
 +
    color: white !important;
 +
    box-shadow:inset 0px 15px 15px -15px #000;
 +
}
 +
 +
div#background-reference {}
 +
#background-reference h1 {
 +
    font-size: 50px;
 +
    color: white!important;
 +
}
 +
#background-reference p{
 +
    padding-top: 20px;
 +
    text-align:justify;
 +
    font-size: 20px;
 +
    text-justify:inter-ideograph;
 +
    color: white!important;
 +
}
 +
#background-reference a {
 +
    text-decoration: none;
 +
    color: white;
 +
}
 +
#background-reference a:hover{
 +
    color: orange;
 +
}
 
</style>
 
</style>
−
</head>
 
  
 +
</head>
 
<body>
 
<body>
 
<!--menu-->
 
<!--menu-->
−
<div id="menu">
+
 
−
<li id="nav">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
+
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
−
<a class="menu1" href="#" style="text-align: right;">☰</a>
+
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 +
 
<ul class="firstmenu" style="float: left">
 
<ul class="firstmenu" style="float: left">
 
 
Line 35: Line 196:
 
<ul id="sub_02">
 
<ul id="sub_02">
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION &amp; DESIGN</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Design" target="_self">DESIGN</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODE BOOK</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODING BOOK</a></li>
+
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
<li class="mainlevel" id="mainlevel_03">
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic_Part"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENTS</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">INFORMATION DESTRUCTION</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">Pre-RNA SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
      <li class="mainlevel" id="mainlevel_04">
+
   
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
+
−
  <ul id="sub_04">
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
+
−
  </ul>
+
−
  </li>
+
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <ul id="sub_05">
 
  <ul id="sub_05">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">MODEL1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">MODEL2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">KILLING MODEL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 69: Line 230:
 
  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 78: Line 240:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 84: Line 245:
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <ul id="sub_08">
 
  <ul id="sub_08">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Human_Practices" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 101: Line 256:
 
</li>
 
</li>
 
</div>
 
</div>
 +
<div class="layer-bottom">
 +
 +
<canvas id="canvas" style="background:#000000"></canvas>
 +
 +
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 1800;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 +
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
context.fillStyle = "rgba(0,0,0,0.2)";
 +
context.fillRect(0,0,W,H);
 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
for(var i=0;i<colunms/2;i++){
 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 +
</script>
 +
 +
           
 +
 +
</div>
 +
<header id="home-banner">
 +
    <div class="row banner">
 +
        <div class="banner-text">
 +
       
 +
           
 +
        </div>
 +
    </div>
 +
</header>
 +
<div class="basicpart-content">
 +
<h1 style="font-size: 65px;color: orange;height: 84px;">Composite parts</h1>
 +
<table class="table" style="
 +
    margin-top: 35px!important;
 +
    font-size: 26px!important;
 +
">
 +
    <thead>
 +
<tr>
 +
  <th style="width: 185px;">Number</th>
 +
<th style="width: 185px;">Name</th>
 +
<th style="">Description</th>
 +
    </tr>
 +
    </thead>
 +
    <tbody>
 +
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542011" style="color:orange;"> BBa_K2542011</a></td>
 +
<td><a href="" style="color: yellow">pRPR1-Antisense-tRPR1</a></td>
 +
 +
<td>
 +
<p style="
 +
    font-size: 26px!important;
 +
">
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This part serves as a key structure in our scheme. This plasmid expresses a small RNA that can specifically bind to the secondary structure of the corresponding stem loop and resolve its structure, which allows the DNA sequence downstream the stem loop to be transcribed. </p></td>
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<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542012" style="color:orange;"> BBa_K2542012</a></td>
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<td><a href="#" style="color: yellow">HA1-pCYC-Stemloop1-EGFP-tCYC1-HA2</a></td>
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This part serves as a lock structure in our scheme, in which EGFP is used as a substitute for our encrypted information, and can be used to verify the function of our lock part. Thus, only when a specific small RNA binds to the stem loop and changes the stem loop secondary structure, EGFP transcription can be initiated and EGFP expression can be achieved. This unit contains six parts: the CYC promoter, STEMLOOP, EGFP coding sequence, the CYC terminator and homology arms HA1 and HA2. HA1 and HA2 act as homology arms to achieve homologous recombination, which can insert the DNA with the lock structure sequence into the yeast genome. The stemloop acts as a lock to protect our encrypted information to be transmitted.</p></td>
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<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542013" style="color:orange;"> BBa_K2542013</a></td>
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<td><a href="" style="color: yellow">pFig2c-hBax-tCYC1</a></td>
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This plasmid allows a-type yeast to sense the pheromone α factor secreted by α-type yeast. The a-type <i>Saccharomyces cerevisiae</i> expresses Bax protein, which can arrest cell cycle and reduce cell density. Therefore, upon induction by α factor the a-type yeast cells carrying this plasmid undergo a decrease in cell density, which can be used to protect our encrypted information. </p></td>
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Latest revision as of 22:35, 17 October 2018

Composite Parts

Composite parts

Number Name Description
BBa_K2542011 pRPR1-Antisense-tRPR1

This part serves as a key structure in our scheme. This plasmid expresses a small RNA that can specifically bind to the secondary structure of the corresponding stem loop and resolve its structure, which allows the DNA sequence downstream the stem loop to be transcribed.

BBa_K2542012 HA1-pCYC-Stemloop1-EGFP-tCYC1-HA2

This part serves as a lock structure in our scheme, in which EGFP is used as a substitute for our encrypted information, and can be used to verify the function of our lock part. Thus, only when a specific small RNA binds to the stem loop and changes the stem loop secondary structure, EGFP transcription can be initiated and EGFP expression can be achieved. This unit contains six parts: the CYC promoter, STEMLOOP, EGFP coding sequence, the CYC terminator and homology arms HA1 and HA2. HA1 and HA2 act as homology arms to achieve homologous recombination, which can insert the DNA with the lock structure sequence into the yeast genome. The stemloop acts as a lock to protect our encrypted information to be transmitted.
BBa_K2542013 pFig2c-hBax-tCYC1

This plasmid allows a-type yeast to sense the pheromone α factor secreted by α-type yeast. The a-type Saccharomyces cerevisiae expresses Bax protein, which can arrest cell cycle and reduce cell density. Therefore, upon induction by α factor the a-type yeast cells carrying this plasmid undergo a decrease in cell density, which can be used to protect our encrypted information.