Difference between revisions of "Team:NEFU China/Composite Part"

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{{NEFU_China}}
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<html lang="en">
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<meta charset="utf-8">
  
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<title>Composite Parts</title>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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    padding: 0px;
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
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</head>
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<h1>Composite Parts</h1>
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<body>
 +
<!--menu-->
  
 +
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
 +
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 +
 +
<ul class="firstmenu" style="float: left">
 +
 +
 +
<li class="mainlevel" id="mainlevel_01">
 +
<a href="https://2018.igem.org/Team:NEFU_China"><img id="home" src="https://static.igem.org/mediawiki/2018/a/a9/T--NEFU_China--_Home.png">HOME</a>
 +
</li>
 +
    <li class="mainlevel" id="mainlevel_02">
 +
<a href="https://2018.igem.org/Team:NEFU_China/Background"><img id="project" src="https://static.igem.org/mediawiki/2018/1/14/T--NEFU_China--_PROJECT.png">PROJECT</a>
 +
<ul id="sub_02">
 +
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
 +
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION &amp; DESIGN</a></li>
 +
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODE BOOK</a></li>
 +
  </ul>
 +
  </li>
 +
<li class="mainlevel" id="mainlevel_03">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENTS</a>
 +
  <ul id="sub_03">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">INFORMATION DESTRUCTION</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">Pre-RNA SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 +
  </ul>
 +
  </li>
 +
 
 +
  <li class="mainlevel" id="mainlevel_05">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 +
  <ul id="sub_05">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">KILLING MODEL</a></li>
 +
  </ul>
 +
  </li>
 +
  <li class="mainlevel" id="mainlevel_06">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Software"><img id="software" src="https://static.igem.org/mediawiki/2018/c/c0/T--NEFU_China--_SOFTWARE.png">SOFTWARE</a>
 +
  <ul id="sub_06">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 +
  </ul>
 +
  </li>
 +
  <li class="mainlevel" id="mainlevel_07">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Attributions"><img id="team" src="https://static.igem.org/mediawiki/2018/5/50/T--NEFU_China--_TEAM.png">TEAM</a>
 +
  <ul id="sub_07">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 +
  </ul>
 +
  </li>
 +
  <li class="mainlevel" id="mainlevel_08" >
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 +
  <ul id="sub_08">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Human_Practices" target="_self">OVERVIEW</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 +
  </ul>
 +
  </li>
 +
 +
</ul>
 +
</li>
 +
</div>
 +
<div class="layer-bottom">
 +
 +
<canvas id="canvas" style="background:#000000"></canvas>
  
−
<p>
+
<script type="text/javascript">
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
−
</p>
+
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 1800;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
−
</div>
 
  
−
<div class="column full_size">
+
var str1 = "ATCG";
−
<div class="highlight decoration_background">
+
                        var str2 = "01";
−
<h3>Note</h3>
+
function draw(){
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
+
context.fillStyle = "rgba(0,0,0,0.2)";
−
</div>
+
context.fillRect(0,0,W,H);
−
</div>
+
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
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 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
  
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
  
 +
draw();
 +
setInterval(draw,60);
 +
};
  
−
<div class="column full_size">
+
</script>
−
<h3>Best Composite Part Special Prize</h3>
+
 +
           
 +
 +
</div>
 +
<header id="home-banner">
 +
    <div class="row banner">
 +
        <div class="banner-text">
 +
       
 +
           
 +
        </div>
 +
    </div>
 +
</header>
 +
<div class="basicpart-content">
 +
<h1 style="font-size: 65px;color: orange;height: 84px;">Composite parts</h1>
 +
<table class="table" style="
 +
    margin-top: 35px!important;
 +
    font-size: 26px!important;
 +
">
 +
    <thead>
 +
<tr>
 +
  <th style="width: 185px;">Number</th>
 +
<th style="width: 185px;">Name</th>
 +
<th style="">Description</th>
 +
    </tr>
 +
    </thead>
 +
    <tbody>
  
−
<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
+
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542011" style="color:orange;"> BBa_K2542011</a></td>
 +
<td><a href="" style="color: yellow">pRPR1-Antisense-tRPR1</a></td>
 +
 +
<td>
 +
<p style="
 +
    font-size: 26px!important;
 +
">
 +
This part serves as a key structure in our scheme. This plasmid expresses a small RNA that can specifically bind to the secondary structure of the corresponding stem loop and resolve its structure, which allows the DNA sequence downstream the stem loop to be transcribed. </p></td>
 +
</tr>  
  
−
<br><br>
+
<tr>
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542012" style="color:orange;"> BBa_K2542012</a></td>
 +
<td><a href="#" style="color: yellow">HA1-pCYC-Stemloop1-EGFP-tCYC1-HA2</a></td>
 +
 +
<td style="
 +
    font-size: 26px!important;
 +
"><p style="
 +
    font-size: 26px!important;
 +
">
 +
This part serves as a lock structure in our scheme, in which EGFP is used as a substitute for our encrypted information, and can be used to verify the function of our lock part. Thus, only when a specific small RNA binds to the stem loop and changes the stem loop secondary structure, EGFP transcription can be initiated and EGFP expression can be achieved. This unit contains six parts: the CYC promoter, STEMLOOP, EGFP coding sequence, the CYC terminator and homology arms HA1 and HA2. HA1 and HA2 act as homology arms to achieve homologous recombination, which can insert the DNA with the lock structure sequence into the yeast genome. The stemloop acts as a lock to protect our encrypted information to be transmitted.</p></td>
 +
</tr>
 +
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542013" style="color:orange;"> BBa_K2542013</a></td>
 +
<td><a href="" style="color: yellow">pFig2c-hBax-tCYC1</a></td>
 +
 +
<td>
 +
<p style="
 +
    font-size: 26px!important;
 +
">
 +
This plasmid allows a-type yeast to sense the pheromone α factor secreted by α-type yeast. The a-type <i>Saccharomyces cerevisiae</i> expresses Bax protein, which can arrest cell cycle and reduce cell density. Therefore, upon induction by α factor the a-type yeast cells carrying this plasmid undergo a decrease in cell density, which can be used to protect our encrypted information. </p></td>
 +
</tr>
 +
 +
</tbody>
 +
</table>
  
 
</div>
 
</div>
 +
 +
</body>
  
 
</html>
 
</html>

Latest revision as of 22:35, 17 October 2018

Composite Parts

Composite parts

Number Name Description
BBa_K2542011 pRPR1-Antisense-tRPR1

This part serves as a key structure in our scheme. This plasmid expresses a small RNA that can specifically bind to the secondary structure of the corresponding stem loop and resolve its structure, which allows the DNA sequence downstream the stem loop to be transcribed.

BBa_K2542012 HA1-pCYC-Stemloop1-EGFP-tCYC1-HA2

This part serves as a lock structure in our scheme, in which EGFP is used as a substitute for our encrypted information, and can be used to verify the function of our lock part. Thus, only when a specific small RNA binds to the stem loop and changes the stem loop secondary structure, EGFP transcription can be initiated and EGFP expression can be achieved. This unit contains six parts: the CYC promoter, STEMLOOP, EGFP coding sequence, the CYC terminator and homology arms HA1 and HA2. HA1 and HA2 act as homology arms to achieve homologous recombination, which can insert the DNA with the lock structure sequence into the yeast genome. The stemloop acts as a lock to protect our encrypted information to be transmitted.
BBa_K2542013 pFig2c-hBax-tCYC1

This plasmid allows a-type yeast to sense the pheromone α factor secreted by α-type yeast. The a-type Saccharomyces cerevisiae expresses Bax protein, which can arrest cell cycle and reduce cell density. Therefore, upon induction by α factor the a-type yeast cells carrying this plasmid undergo a decrease in cell density, which can be used to protect our encrypted information.