Difference between revisions of "Team:NEFU China/Basic Part"

 
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Line 1: Line 1:
 
<html lang="en">
 
<html lang="en">
−
 
 
<head>
 
<head>
 
<meta charset="utf-8">
 
<meta charset="utf-8">
−
<meta http-equiv="X-UA-Compatible" content="IE=edge">
 
  
 
<title>Basic Parts</title>
 
<title>Basic Parts</title>
−
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-members-foot&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-style-basicparts&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-style-basicparts&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 +
 
<style>
 
<style>
−
 
 
#top_title, #sideMenu{
 
#top_title, #sideMenu{
 
     display: none;
 
     display: none;
Line 22: Line 19:
 
}
 
}
 
#foot-title h3 {
 
#foot-title h3 {
−
font-size:18px!important;
+
    color:rgba(165,165,165,1.00);
 +
    line-height: 20px;
 +
    font-size: 18px;
 +
}
 +
#menu li ul li:hover ul{
 +
    background:rgba(0,0,0,0.75)!important;
 +
}
 +
li#mainlevel_01 a {
 +
    color: #FFE5B5!important;
 +
 +
}
 +
li#mainlevel_01 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_02 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_02 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_03 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_03 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_05 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_05 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_06 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_06 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_07 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_07 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_08 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_08 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
#menu li ul li ul li a:hover {
 +
    color: rgba(0,223,252,1);
 +
    border-top: dotted 1px rgba(255,255,255,0.91);
 +
    border-bottom: dotted 1px rgba(255,255,255,0.91);
 +
    background: rgba(0,223,252,.02);
 +
}
 +
#nav .mainlevel a {
 +
    color: black;
 +
    text-decoration:none;
 +
    line-height:32px;
 +
    display:block;
 +
    padding:0 5px;
 +
    font-size: 25px!important;
 +
font-family: 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif, 'Apple Color Emoji', 'Segoe UI Emoji', 'Segoe UI Symbol', 'Noto Color Emoji' !important;
 +
}
 +
.layer-bottom {
 +
z-index: -2;
 +
position: absolute;
 +
margin-top: 36px!important;
 +
}
 +
.basicpart-content {
 +
    padding-left: 150px;
 +
    padding-right: 150px;
 +
    padding-top: 40px;
 +
}
 +
 
 +
table.table {
 +
    background-color: transparent;
 +
}
 +
 
 +
.table th {
 +
    font-size: 22px;
 +
}
 +
 
 +
.table tbody {
 +
    background-color: transparent!important;
 +
}
 +
 
 +
.table td {
 +
    background-color: transparent;
 +
    font-size: 20px;
 +
    vertical-align: middle!important;
 +
    line-height: 27px;
 +
}
 +
 
 +
table.table {
 +
    background-color: rgb(0,0,0,0.6)!important;
 +
    color: white;
 +
}
 +
#background-reference {
 +
    padding-top: 50px;
 +
    padding-left: 150px;
 +
    padding-right:150px;
 +
    border:2px;
 +
    border-style: none;
 +
    font-family:"Open Sans" !important;
 +
    font-size:15px;
 +
    color: white !important;
 +
    box-shadow:inset 0px 15px 15px -15px #000;
 +
}
 +
 
 +
div#background-reference {}
 +
#background-reference h1 {
 +
    font-size: 50px;
 +
    color: white!important;
 +
}
 +
#background-reference p{
 +
    padding-top: 20px;
 +
    text-align:justify;
 +
    font-size: 20px;
 +
    text-justify:inter-ideograph;
 +
    color: white!important;
 +
}
 +
#background-reference a {
 +
    text-decoration: none;
 +
    color: white;
 +
}
 +
#background-reference a:hover{
 +
    color: orange;
 
}
 
}
 
</style>
 
</style>
−
</head>
 
  
 +
</head>
 
<body>
 
<body>
 
<!--menu-->
 
<!--menu-->
−
<div id="menu">
+
 
−
<li id="nav">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
+
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
 +
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 
 
 
<ul class="firstmenu" style="float: left">
 
<ul class="firstmenu" style="float: left">
Line 42: Line 196:
 
<ul id="sub_02">
 
<ul id="sub_02">
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION &amp; DESIGN</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Design" target="_self">DESIGN</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODE BOOK</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODING BOOK</a></li>
+
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
<li class="mainlevel" id="mainlevel_03">
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic_Part"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENTS</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">INFORMATION DESTRUCTION</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">Pre-RNA SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
      <li class="mainlevel" id="mainlevel_04">
+
   
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
+
−
  <ul id="sub_04">
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
+
−
  </ul>
+
−
  </li>
+
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <ul id="sub_05">
 
  <ul id="sub_05">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">MODEL1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">MODEL2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">KILLING MODEL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 76: Line 230:
 
  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 85: Line 240:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 91: Line 245:
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <ul id="sub_08">
 
  <ul id="sub_08">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Human_Practices" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 108: Line 256:
 
</li>
 
</li>
 
</div>
 
</div>
−
<div id="lead">
+
<div class="layer-bottom">
−
    <div id="lead-content">
+
−
        <h1>Basic Parts</h1>
+
<canvas id="canvas" style="background:#000000"></canvas>
−
        <h2>Basic Parts's introduction.</h2>
+
 
−
    </div>
+
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 3000;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 
 +
 
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
context.fillStyle = "rgba(0,0,0,0.2)";
 +
context.fillRect(0,0,W,H);
 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
for(var i=0;i<colunms/2;i++){
 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 
 +
</script>
 +
 +
           
 +
 
</div>
 
</div>
−
 
+
<header id="home-banner">
−
<div id="about" style="box-shadow: inset 0px 15px 15px -15px green;">
+
    <div class="row banner">
−
<div class="container">
+
        <div class="banner-text">
−
<div class="row">
+
           
−
                <div class="col-md-4">
+
           
−
                    <h2 class="heading">Basic Parts</h2>
+
        </div>
−
                </div>
+
−
                <div class="col-md-8">
+
−
                    <p>
+
−
                        IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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                    </p>
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                </div>
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        </div>
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     </div>
 
     </div>
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</div>
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</header>
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<hr style="text-align: center;margin-left: 150px;margin-right: 150px;">
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<div class="basicpart-content">
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<div class="humanpractice">
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<h1 style="font-size: 65px;color: orange;height: 84px;">Basic parts</h1>
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    <h2 class="heading">Basic Parts</h2>
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<table class="table" style="
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    <div id="title1" class="humanpractice-block">
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    margin-top: 35px!important;
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        <h3>title：111111111111111</h3>
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">
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    <thead>
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        <p>
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<tr>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
+
  <th style="width: 185px;">Number</th>
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        </p>
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<th style="width: 185px;">Name</th>
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    </div>
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<th style="">Description</th>
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    <div id="title2" class="humanpractice-block">
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    </tr>
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<h3>title：222222222222222</h3>
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    </thead>
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    <tbody>
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<p>
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<tr>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
+
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1154001" style="color:orange;">BBa_K1154001</a></td>
−
        </p>
+
<td><a href="#" style="color: yellow">pFUS1</a></td>
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    </div>
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−
<div id="title3" class="humanpractice-block">
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<h3>title：333333333333333</h3>
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<p>
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−
            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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−
        </p>
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−
    </div>
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<div id="title4" class="humanpractice-block">
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<h3>title：444444444444444</h3>
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<p>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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−
        </p>
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    </div>
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<div id="title5" class="humanpractice-block">
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<h3>title：555555555555555</h3>
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<p>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
+
−
        </p>
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    </div>
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</div>
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<br>
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<br>
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<br>
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<div class="foot">
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<div id="foot-title">
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<table>
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<td>
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<h1>Userfull links</h1>
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<h2>
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<a href="https://2018.igem.org/Team:NEFU_China">Home</a>
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&nbsp;&nbsp;&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Model">Model</a>
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                        &nbsp;&nbsp;
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                        <a href="https://2018.igem.org/Team:NEFU_China/Description">Project</a>
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&nbsp;&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Software">Software</a>
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    &nbsp;&nbsp;
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    </h2>
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<h2>
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<a href="https://2018.igem.org/Team:NEFU_China/Basic_Part">Parts</a>
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&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Members">Teams</a>
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&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Lock_Key">Results</a>
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&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Notebook">Notebook</a>
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                        &nbsp;&nbsp;
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</h2>
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<h2>
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<a href="https://2018.igem.org/Team:NEFU_China/Human_Practices">Human &nbsp;Practice</a>
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</h2>
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</td>
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<td style="padding-left: 70px">
+
−
<h1>Follow us</h1>
+
−
<img alt="facebook" src="https://static.igem.org/mediawiki/2018/b/b5/T--NEFU_China--facebook.png">
+
−
&nbsp;&nbsp;&nbsp;
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        <img alt="twitter" src="https://static.igem.org/mediawiki/2018/3/36/T--NEFU_China--twitter.png">
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&nbsp;&nbsp;&nbsp;
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<img alt="wehchat" src="https://static.igem.org/mediawiki/2018/c/ca/T--NEFU_China--wechat.png">
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        <br>
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        <img alt="facebook" src="https://static.igem.org/mediawiki/2018/b/b5/T--NEFU_China--facebook.png">
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&nbsp;&nbsp;&nbsp;
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        <img alt="twitter" src="https://static.igem.org/mediawiki/2018/3/36/T--NEFU_China--twitter.png">
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&nbsp;&nbsp;&nbsp;
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<img alt="wehchat" src="https://static.igem.org/mediawiki/2018/c/ca/T--NEFU_China--wechat.png">
+
 
 
−
</td>
+
<td><p>
−
<td style="padding-left: 70px">
+
Our team were aimed at adjusting the expression level of any existing promoter. Thus, we modified the Kozak sequence of the promoter in the plasmid <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K775004" style="color:orange;">BBa_K775004</a>, a part from the University of the TU-Delft 2012 iGEM team. Our goal is to reduce the expression efficacy of this vector, and make it suitable to express a suicide gene for information destruction. To reduce the expression efficiency of this part in yeast, we modified the Kozak sequence by mutating the sequence “AAAAAA” to “GCCACC”. After that, our information destruction system was built and kill the information host yeast in a desired period of time. See more details in the basic part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542014" style="color:orange;">BBa_K2542014</a>. We used the enhanced green fluorescent protein (EGFP) to test the effect of this modification. The&nbsp;generated plasmid&nbsp;allows&nbsp;a&nbsp;type&nbsp;yeast&nbsp;to&nbsp;sense&nbsp;the α factor&nbsp;secreted&nbsp;by&nbsp;α-type&nbsp;yeast&nbsp;and&nbsp;express&nbsp;EGFP. Thus, when the promoter was activated by &nbsp;factor&nbsp;secreted&nbsp;by&nbsp;α-type&nbsp;yeast, the EGFP would be expressed. Thus, the EGFP expression would be the standard to tell the strength of our improved promoter.</p></td>
−
<h1>Contact us</h1>
+
</tr>
−
<h3>iGEM-NEFU_China2018</h3>
+
−
<h3>Email: hexinglu@nefu.edu.cn</h3>
+
<tr>
−
<h3>No.26 Hexing Road, Xiangfang <br>District, Harbin, Heilongjiang <br>Province 150000</h3>
+
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1829002" style="color:orange;"> BBa_K1829002</a></td>
−
</td>
+
<td><a href="" style="color: yellow">pFig2c</a></td>
−
</tr>
+
−
</table>
+
<td>
−
</div>
+
<p>
−
<div id="foot-declare">
+
Our team attempted to verify the strengthen of a promoter called Fig2c in a part of <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1829002" style="color:orange;">BBa_K1829002</a> from the iGEM 2015 UCSF team. For this purpose, we inserted the pFig2c promoter and EGFP coding sequence into the pesc-ura plasmid, and transferred it into a-type <i>Saccharomyces cerevisiae</i>. The expression of the Fig2c promoter was induced by adding α mating  factor. When the Fig2c promoter is induced, EGFP is expressed. In this way, we can detect the strengthen of the Fig2c promoter using EGFP as a reporter. </p></td>
−
<p>
+
</tr>  
−
+
−
</p>
+
                <tr>
−
</div>
+
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542000" style="color:orange;"> BBa_K2542000</a></td>
−
</div>
+
<td><a href="#" style="color: yellow">Riboswitch1</a></td>
−
   
+
 +
<td><p>
 +
A type of riboswitch, onstitutive promoter derived from the yeast, and it can initiate medium-intensity gene expression. We use it to control the transcription of our information.</p></td>
 +
</tr>
 +
 
 +
                <tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542001" style="color:orange;"> BBa_K2542001</a></td>
 +
<td><a href="#" style="color: yellow">Riboswitch2</a></td>
 +
 +
<td><p>
 +
A type of riboswitch, onstitutive promoter derived from the yeast, and it can initiate medium-intensity gene expression. We use it to control the transcription of our information.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542002" style="color:orange;"> BBa_K2542002</a></td>
 +
<td><a href="#" style="color: yellow">Riboswitch3</a></td>
 +
 +
<td><p>
 +
A type of riboswitch, onstitutive promoter derived from the yeast, and it can initiate medium-intensity gene expression. We use it to control the transcription of our information.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542003" style="color:orange;"> BBa_K2542003</a></td>
 +
<td><a href="#" style="color: yellow">Key1</a></td>
 +
 +
<td><p>
 +
A Small RNA which can change the secondary structure of the riboswitch (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542000" style="color:orange;">BBa_K2542000</a>). It can transcribe a small RNA that adapts to the riboswitch (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542000" style="color:orange;">BBa_K2542000</a>) and alters its secondary structure so that transcription is not blocked by the riboswitch.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542004" style="color:orange;"> BBa_K2542004</a></td>
 +
<td><a href="#" style="color: yellow">Key2</a></td>
 +
 +
<td><p>
 +
A Small RNA which can change the secondary structure of the riboswitch (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542001" style="color:orange;">BBa_K2542001</a>). It can transcribe a small RNA that adapts to the riboswitch （<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542001" style="color:orange;">BBa_K2542001</a>） and alters its secondary structure so that transcription is not blocked by the riboswitch.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542005" style="color:orange;"> BBa_K2542005</a></td>
 +
<td><a href="#" style="color: yellow">Key3</a></td>
 +
 +
<td><p>
 +
A Small RNA which can change the secondary structure of the riboswitch (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542002" style="color:orange;">BBa_K2542002</a>). It can transcribe a small RNA that adapts to the riboswitch （<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542002" style="color:orange;">BBa_K2542002</a>） and alters its secondary structure so that transcription is not blocked by the riboswitch.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542006" style="color:orange;"> BBa_K2542006</a></td>
 +
<td><a href="#" style="color: yellow">pUra</a></td>
 +
 +
<td><p>
 +
This is a constitutive promoter that initiates gene expression in yeast.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542007" style="color:orange;"> BBa_K2542007</a></td>
 +
<td><a href="#" style="color: yellow">Homologous arm1</a></td>
 +
 +
<td><p>
 +
It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542008" style="color:orange;">BBa_K2542008</a>.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542008" style="color:orange;"> BBa_K2542008</a></td>
 +
<td><a href="#" style="color: yellow">Homologous arm2</a></td>
 +
 +
<td><p>
 +
It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542007" style="color:orange;">BBa_K2542007</a>.</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542009" style="color:orange;"> BBa_K2542009</a></td>
 +
<td><a href="#" style="color: yellow">pRPR1</a></td>
 +
 +
<td><p>
 +
It' a promoter capable of being recognized by RNA polymerase III, and the RNA driven by it does not add 5' cap and 3' poly A tail. This is a promoter and is usually used with the RPR1 terminator (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542010" style="color:orange;">BBa_K2542010</a>).</p></td>
 +
</tr>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542010" style="color:orange;"> BBa_K2542010</a></td>
 +
<td><a href="#" style="color: yellow">tRPR1</a></td>
 +
 +
<td><p>
 +
This is a terminator and is usually used with the RPR1 promoter (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542009" style="color:orange;">BBa_K2542009</a>).</p></td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
 +
</div>
 +
 
 +
 
 
</body>
 
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Latest revision as of 23:19, 17 October 2018

Basic Parts

Basic parts

Number Name Description
BBa_K1154001 pFUS1

Our team were aimed at adjusting the expression level of any existing promoter. Thus, we modified the Kozak sequence of the promoter in the plasmid BBa_K775004, a part from the University of the TU-Delft 2012 iGEM team. Our goal is to reduce the expression efficacy of this vector, and make it suitable to express a suicide gene for information destruction. To reduce the expression efficiency of this part in yeast, we modified the Kozak sequence by mutating the sequence “AAAAAA” to “GCCACC”. After that, our information destruction system was built and kill the information host yeast in a desired period of time. See more details in the basic part BBa_K2542014. We used the enhanced green fluorescent protein (EGFP) to test the effect of this modification. The generated plasmid allows a type yeast to sense the α factor secreted by α-type yeast and express EGFP. Thus, when the promoter was activated by  factor secreted by α-type yeast, the EGFP would be expressed. Thus, the EGFP expression would be the standard to tell the strength of our improved promoter.
BBa_K1829002 pFig2c

Our team attempted to verify the strengthen of a promoter called Fig2c in a part of BBa_K1829002 from the iGEM 2015 UCSF team. For this purpose, we inserted the pFig2c promoter and EGFP coding sequence into the pesc-ura plasmid, and transferred it into a-type Saccharomyces cerevisiae. The expression of the Fig2c promoter was induced by adding α mating factor. When the Fig2c promoter is induced, EGFP is expressed. In this way, we can detect the strengthen of the Fig2c promoter using EGFP as a reporter.

BBa_K2542000 Riboswitch1

A type of riboswitch, onstitutive promoter derived from the yeast, and it can initiate medium-intensity gene expression. We use it to control the transcription of our information.
BBa_K2542001 Riboswitch2

A type of riboswitch, onstitutive promoter derived from the yeast, and it can initiate medium-intensity gene expression. We use it to control the transcription of our information.
BBa_K2542002 Riboswitch3

A type of riboswitch, onstitutive promoter derived from the yeast, and it can initiate medium-intensity gene expression. We use it to control the transcription of our information.
BBa_K2542003 Key1

A Small RNA which can change the secondary structure of the riboswitch (BBa_K2542000). It can transcribe a small RNA that adapts to the riboswitch (BBa_K2542000) and alters its secondary structure so that transcription is not blocked by the riboswitch.
BBa_K2542004 Key2

A Small RNA which can change the secondary structure of the riboswitch (BBa_K2542001). It can transcribe a small RNA that adapts to the riboswitch （BBa_K2542001） and alters its secondary structure so that transcription is not blocked by the riboswitch.
BBa_K2542005 Key3

A Small RNA which can change the secondary structure of the riboswitch (BBa_K2542002). It can transcribe a small RNA that adapts to the riboswitch （BBa_K2542002） and alters its secondary structure so that transcription is not blocked by the riboswitch.
BBa_K2542006 pUra

This is a constitutive promoter that initiates gene expression in yeast.
BBa_K2542007 Homologous arm1

It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is BBa_K2542008.
BBa_K2542008 Homologous arm2

It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is BBa_K2542007.
BBa_K2542009 pRPR1

It' a promoter capable of being recognized by RNA polymerase III, and the RNA driven by it does not add 5' cap and 3' poly A tail. This is a promoter and is usually used with the RPR1 terminator (BBa_K2542010).
BBa_K2542010 tRPR1

This is a terminator and is usually used with the RPR1 promoter (BBa_K2542009).