Difference between revisions of "Team:BJRS China/Human Practices"

 
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<div class="navbar-header"><a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="120px" height="120px" style="position:relative;top:0px;left:90%;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BJRS_China--logo2.jpeg"width="1200px" height="50px"style="position:relative;top:-114px;left:10px;"></a></div>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Background">BACKGROUND</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Design">DESIGN</a></li> 
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Results">RESULTS</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Improve">IMPROVE</a></li>
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        <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Human_Practices">Human Practices</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Protocol">PROTOCOL</a></li>
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      </ul>
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    </div>
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</nav>
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<div style="padding-left:240px;padding-right:240px">
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  <h1 class="text-center"><strong> Human Practices</strong></h1>
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  <div style="text-align:center;font-size:25px">
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    <a href="#O">Overview/</a>
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    <a href="#IHP">integrated Human Practice/</a>
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    <a href="https://2018.igem.org/Team:BJRS_China/Public_Engagement">Public Engagement</a>
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  </div>
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  <div>
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  <a id="O"><br/></a>
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  <h2 class="text-center">Overview</h2>
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    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;Our goal is to increase the oxygen carrying capacity of engineering bacteria. We expect to increase the yield of engineered bacteria. If realized, the development of fermentation industry can be promoted. In order to get more information about our project and guide our project, we went to Bluepha to do research and interview.
 +
We also hope that by participating in the iGEM competition, we can have an impact on our community and the people around us. So we launched a questionnaire in the name of BJRS, and through the promotion of BJRS public name, to investigate public attitudes and understanding of genetically modified foods. A total of 675 feedbacks were received, ranging from under 12 to over 65, covering a wide range of educational qualifications and jobs. The results show that the public's understanding of GM is still inadequate, and the phenomenon of blind resistance is serious. So we carried out a lecture on Synthetic biology in Beijing Jianhua Experimental School.</font></p>
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<figure class="text-center">
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                <img src="https://static.igem.org/mediawiki/2018/5/54/T--BJRS_China--hp_tank.jpeg"width="70%">
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                <figcaption>
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                    Observing the fermentation tank and listening to the introduction
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                </figcaption>
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            </figure>
  
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  <a id="IHP"><br/></a>
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    <h2 class="text-center"> Integrated Human Practices</h2>
  
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    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;Bluepha is a bio fermentation company. Its main products are bio plastics. In the company, we practically experienced biological plastic products, and learned the fermentation tank and fermentation process, also, interviewed the employees. The following is the interview record.</font></p>
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    <h4>Here Are Some <i>Q&As </i> </h4>
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: What is the most pressing issue in fermetation process？</font></p>
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<h1>Human Practices</h1>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: The biggest issue is disinfection, which acounts for almost half of the excutive costs in most circumstances. Contamination of other bacteria lead to waste and disposal of entire fernetautib cylinder.</font></p>
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<p>
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At iGEM we believe societal considerations should be upfront and integrated throughout the design and execution of synthetic biology projects. “Human Practices” refers to iGEM teams’ efforts to actively consider how the world affects their work and the work affects the world. Through your Human Practices activities, your team should demonstrate how you have thought carefully and creatively about whether your project is responsible and good for the world. We invite you to explore issues relating (but not limited) to the ethics, safety, security, and sustainability of your project, and to show how this exploration feeds back into your project purpose, design and execution.
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: What are the other pressing issues?</font></p>
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</p>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: Yield of engineered strain and downstream processing.</font></p>
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: What is the current way of oxygen supply?</font></p>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: Currently we bubble sterilised air to the cylinder and use blades to stir for homogenous distribution of air.</font></p>
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<p>For more information, please see the <a href="https://2018.igem.org/Human_Practices">Human Practices Hub</a>. There you will find:</p>
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: Have you ever think of other ways to increase oxygen capacity?</font></p>
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<ul>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: Not as I know it. The oxygen solubility in water gives a upper limit for oxygen capacity.</font></p>
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<li> an <a href="https://2018.igem.org/Human_Practices/Introduction">introduction</a> to Human Practices at iGEM </li>
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<li>tips on <a href="https://2018.igem.org/Human_Practices/How_to_Succeed">how to succeed</a> including explanations of judging criteria and advice about how to conduct and document your Human Practices work</li>
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: What is the current fermetation rate?</font></p>
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<li>descriptions of <a href="https://2018.igem.org/Human_Practices/Examples">exemplary work</a> to inspire you</li>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: It usually takes two to three days for a typical cylinder to finish fermetation. The downstream processes cost at least another two to three days.</font></p>
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<li>links to helpful <a href="https://2018.igem.org/Human_Practices/Resources">resources</a></li>
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<li>And more! </li>
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: What factors affect the process?</font></p>
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</ul>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: The main factor is the nature of the engineered strain we chosen. Other factors include the product we desire, and the medium we use.</font></p>
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    <p class="text-center" style="line-height:30px"><font size="3">    Q: How to select engineered strains in Bluepha?</font></p>
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    <p class="text-center" style="line-height:30px"><font size="3">    A: Currently, we use a holamonas strain which survives in high salt level. This potentially prevents from contamination of other bacerium as they are not able to grow in such an extreme condition.</font></p>
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<br/>
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<figure class="text-center">
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<p>On this page, your team should document all of your Human Practices work and activities. You should write about the Human Practices topics you considered in your project, document any activities you conducted to explore these topics (such as engaging with experts and stakeholders), describe why you took a particular approach (including referencing any work you built upon), and explain if and how you integrated takeaways from your Human Practices work back into your project purpose, design and/or execution. </p>
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                <img src="https://static.igem.org/mediawiki/2018/e/e0/T--BJRS_China--hp_interview.jpeg"width="70%">
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                <figcaption>
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<p>If your team has gone above and beyond in work related to safety, then you should document this work on your Safety wiki page and provide a description and link on this page. If your team has developed education and public engagement efforts that go beyond a focus on your particular project, and for which would like to nominate your team for the Best Education and Public Engagement Special Prize, you should document this work on your Education and Education wiki page and provide a description and link here. </p>
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                    Interviewing with the manager
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                </figcaption>
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            </figure>
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<br/>
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    <h4>&emsp;&emsp;After on-the-spot visits and data consulting, the members of the group concluded that there were two main indicators for the evaluation of fermentation effect.</h4>
 +
    <p class="text-center" style="line-height:30px"><font size="3">  <b> 1. fermentation rate / fermentation cycle of bacteria</b></font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;The fermentation of bacteria can be roughly divided into two processes. According to our understanding of fermentation process, the total cycle is about 48-72 hours. The first part is the growth period of bacteria, which lasts about 24-36 hours. When the bacteria grow to the plateau stage (i.e. no nutrient supply is needed for growth and development), it enters the second stage, that is, the production of the target product stage, which lasts about 24-56 hours, the production capacity of bacteria reaches the limit.</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;The fermentation rate of bacteria is mainly affected by dissolved oxygen. In the process of fermentation, the content of dissolved oxygen is restricted by two factors.</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    （1） Type of tank and fan blade</font></p>
 +
    <p class="text-center"><font size="3">    &emsp;&emsp;Different fermentation tanks and fan blades will affect the contact degree and mixing uniformity of bacteria with various nutrients and oxygen, and then affect the growth and production efficiency of bacteria. Therefore, suitable fermentor should be selected during fermentation.</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    （2） pH value</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;In the process of bacterial growth, respiration will produce lactic acid and other organic acids, resulting in less and less pH in the tank, fermentation efficiency gradually reduced. Therefore, it is necessary to adjust the pH value every half hour in the fermentation process to ensure that the fermentation efficiency does not decrease.</font></p>
 +
<br/>
 +
    <p class="text-center" style="line-height:30px"><font size="3">  <b> 2. pour rate / recovery rate</b></font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;After fermentation, the material in the tank needs to be recovered. Not all fermenters can produce a large number of target products, some even can not get the target products. Therefore, in this case, the concept of "pouring rate" has emerged, that is, the number of successful tanks as a proportion of the total number of tanks. This indicator is affected by two factors.</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    （1）improper disinfection</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;before fermentation, the fermentor must be sterilized once. If the disinfection is not complete, the mixed bacteria will be mixed into the tank and grow with the target strain. Some of the more viable strains will grab nutrients and oxygen, resulting in a serious decline in the survival and dry weight of the target strains.</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    （2） pH value</font></p>
 +
    <p class="text-center" style="line-height:30px"><font size="3">    &emsp;&emsp;If pH value is not suitable for the growth of the target bacteria, it will also lead to a very small amount of bacteria, the rate of tank pouring is low.</font></p>
 
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                <figcaption>
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<p>The iGEM judges will review this page to assess whether you have met the Silver and/or Gold medal requirements based on the Integrated Human Practices criteria listed below. If you nominate your team for the <a href="https://2018.igem.org/Judging/Awards">Best Integrated Human Practices Special Prize</a> by filling out the corresponding field in the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>, the judges will also review this page to consider your team for that prize.
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                    Photo with the company's manager
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<h3>Silver Medal Criterion #3</h3>
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<p>Convince the judges you have thought carefully and creatively about whether your work is responsible and good for the world. Document how you have investigated these issues and engaged with your relevant communities, why you chose this approach, and what you have learned. Please note that surveys will not fulfill this criteria unless you follow scientifically valid methods. </p>
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<h3>Gold Medal Criterion #1</h3>
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<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the purpose, design and/or execution of your project. Document how your project has changed based upon your human practices work.
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<p>To compete for the Best Integrated Human Practices prize, please describe your work on this page and also fill out the description on the judging form. </p>
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<p>How does your project affect society and how does society influence the direction of your project? How might ethical considerations and stakeholder input guide your project purpose and design and the experiments you conduct in the lab? How does this feedback enter into the process of your work all through the iGEM competition? Document a thoughtful and creative approach to exploring these questions and how your project evolved in the process to compete for this award!</p>
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Latest revision as of 23:51, 17 October 2018

BJRS

Human Practices


Overview

  Our goal is to increase the oxygen carrying capacity of engineering bacteria. We expect to increase the yield of engineered bacteria. If realized, the development of fermentation industry can be promoted. In order to get more information about our project and guide our project, we went to Bluepha to do research and interview. We also hope that by participating in the iGEM competition, we can have an impact on our community and the people around us. So we launched a questionnaire in the name of BJRS, and through the promotion of BJRS public name, to investigate public attitudes and understanding of genetically modified foods. A total of 675 feedbacks were received, ranging from under 12 to over 65, covering a wide range of educational qualifications and jobs. The results show that the public's understanding of GM is still inadequate, and the phenomenon of blind resistance is serious. So we carried out a lecture on Synthetic biology in Beijing Jianhua Experimental School.


Observing the fermentation tank and listening to the introduction





Integrated Human Practices

  Bluepha is a bio fermentation company. Its main products are bio plastics. In the company, we practically experienced biological plastic products, and learned the fermentation tank and fermentation process, also, interviewed the employees. The following is the interview record.

Here Are Some Q&As

Q: What is the most pressing issue in fermetation process？

A: The biggest issue is disinfection, which acounts for almost half of the excutive costs in most circumstances. Contamination of other bacteria lead to waste and disposal of entire fernetautib cylinder.


Q: What are the other pressing issues?

A: Yield of engineered strain and downstream processing.


Q: What is the current way of oxygen supply?

A: Currently we bubble sterilised air to the cylinder and use blades to stir for homogenous distribution of air.


Q: Have you ever think of other ways to increase oxygen capacity?

A: Not as I know it. The oxygen solubility in water gives a upper limit for oxygen capacity.


Q: What is the current fermetation rate?

A: It usually takes two to three days for a typical cylinder to finish fermetation. The downstream processes cost at least another two to three days.


Q: What factors affect the process?

A: The main factor is the nature of the engineered strain we chosen. Other factors include the product we desire, and the medium we use.


Q: How to select engineered strains in Bluepha?

A: Currently, we use a holamonas strain which survives in high salt level. This potentially prevents from contamination of other bacerium as they are not able to grow in such an extreme condition.


Interviewing with the manager

  After on-the-spot visits and data consulting, the members of the group concluded that there were two main indicators for the evaluation of fermentation effect.

1. fermentation rate / fermentation cycle of bacteria

  The fermentation of bacteria can be roughly divided into two processes. According to our understanding of fermentation process, the total cycle is about 48-72 hours. The first part is the growth period of bacteria, which lasts about 24-36 hours. When the bacteria grow to the plateau stage (i.e. no nutrient supply is needed for growth and development), it enters the second stage, that is, the production of the target product stage, which lasts about 24-56 hours, the production capacity of bacteria reaches the limit.

  The fermentation rate of bacteria is mainly affected by dissolved oxygen. In the process of fermentation, the content of dissolved oxygen is restricted by two factors.

（1） Type of tank and fan blade

  Different fermentation tanks and fan blades will affect the contact degree and mixing uniformity of bacteria with various nutrients and oxygen, and then affect the growth and production efficiency of bacteria. Therefore, suitable fermentor should be selected during fermentation.

（2） pH value

  In the process of bacterial growth, respiration will produce lactic acid and other organic acids, resulting in less and less pH in the tank, fermentation efficiency gradually reduced. Therefore, it is necessary to adjust the pH value every half hour in the fermentation process to ensure that the fermentation efficiency does not decrease.


2. pour rate / recovery rate

  After fermentation, the material in the tank needs to be recovered. Not all fermenters can produce a large number of target products, some even can not get the target products. Therefore, in this case, the concept of "pouring rate" has emerged, that is, the number of successful tanks as a proportion of the total number of tanks. This indicator is affected by two factors.

（1）improper disinfection

  before fermentation, the fermentor must be sterilized once. If the disinfection is not complete, the mixed bacteria will be mixed into the tank and grow with the target strain. Some of the more viable strains will grab nutrients and oxygen, resulting in a serious decline in the survival and dry weight of the target strains.

（2） pH value

  If pH value is not suitable for the growth of the target bacteria, it will also lead to a very small amount of bacteria, the rate of tank pouring is low.

Photo with the company's manager