Difference between revisions of "Team:BJRS China/InterLab"

 
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   <div class="top container-fluid" style="position:fixed; z-index:999; top:0;">
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<div class="navbar-header"> <a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="100px" height="100px" style="position:relative;top:-12px;left:20px;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/8/8b/HQ_page_logo.jpg" width="100px" height="100px" style="position:relative;top:-115px;left: -125px" class="img-fluid rounded"></a>
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<div class="navbar-header"><a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="120px" height="120px" style="position:relative;top:0px;left:90%;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BJRS_China--logo2.jpeg"width="1200px" height="50px"style="position:relative;top:-114px;left:10px;"></a>
 
</div>
 
</div>
 
     <div>
 
     <div>
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a>
 
           <ul class="dropdown-menu">
 
           <ul class="dropdown-menu">
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             <li><a href="https://2018.igem.org/Team:BJRS_China">BACKGROUND</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Background">BACKGROUND</a></li>
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             <li><a href="#">PROJECT</a></li>
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<li><a href="https://2018.igem.org/Team:BJRS_China/Design">DESIGN</a></li> 
 +
             <li><a href="https://2018.igem.org/Team:BJRS_China/Results">RESULTS</a></li>
 
             <li><a href="https://2018.igem.org/Team:BJRS_China/Improve">IMPROVE</a></li>
 
             <li><a href="https://2018.igem.org/Team:BJRS_China/Improve">IMPROVE</a></li>
 +
<li><a href="https://2018.igem.org/Team:BJRS_China/InterLab">INTERLAB</a></li>
 +
<li><a href="https://2018.igem.org/Team:BJRS_China/Demonstrate">DEMONSTRATE</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> EXPERIMENT <b class="caret"></b> </a>
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         <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li>
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          <ul class="dropdown-menu">
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            <li><a href="#">EXPERIMENT</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/InterLab">INTERLAB</a></li>
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          </ul>
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        </li>
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        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> MODEL <b class="caret"></b> </a>
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          <ul class="dropdown-menu">
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Measurement">MEASUREMENT</a></li>
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          </ul>
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        </li>
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a>
 
           <ul class="dropdown-menu">
 
           <ul class="dropdown-menu">
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Human_Practices">HUMAN PRACTICE</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Human_Practices">Human Practices</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Public_Engagement">ENGAGEMENT</a></li>
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            <li><a href="https://2018.igem.org/Team:BJRS_China/Public_Engagement">Public Engagement</a></li>
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            <li><a href="#">GOLD INTERGRATED</a></li>
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           </ul>
 
           </ul>
 
         </li>
 
         </li>
−
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> DEMONSTRATE <b class="caret"></b> </a>
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ACHIEVEMENTS <b class="caret"></b> </a>
 
           <ul class="dropdown-menu">
 
           <ul class="dropdown-menu">
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Demonstrate">DEMONSTRATE</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Parts">PARTS</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Applied_Design">APPLIED DESIGN</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Judging_Criteria">JUDGING CRITERIA</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
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         <li><a href="#">SAFETY</a></li>
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         <li><a href="https://2018.igem.org/Team:BJRS_China/Safety">SAFETY</a></li>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ATTRIBUTION <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ATTRIBUTION <b class="caret"></b> </a>
 
           <ul class="dropdown-menu">
 
           <ul class="dropdown-menu">
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         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <b class="caret"></b> </a>
 
         <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <b class="caret"></b> </a>
 
           <ul class="dropdown-menu">
 
           <ul class="dropdown-menu">
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             <li><a href="#">MEMBERS</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Members">MEMBERS</a></li>
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             <li><a href="#">SCHOOLS</a></li>
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             <li><a href="https://2018.igem.org/Team:BJRS_China/Notebooks">NOTEBOOKS</a></li>
 +
<li><a href="https://2018.igem.org/Team:BJRS_China/Protocol">PROTOCOL</a></li>
 +
<li><a href="https://2018.igem.org/Team:BJRS_China/Acknowledgement">ACKNOWLEDGEMENT</a></li>
 
           </ul>
 
           </ul>
 
         </li>
 
         </li>
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       <section class = "interlab">
 
       <section class = "interlab">
 
         <!--Title-->
 
         <!--Title-->
−
       <h1 style = "text-align: center;color:white">InterLab</h1>
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       <h1 style = "text-align: center;color:black">InterLab</h1>
  
 
       <hr class="line">
 
       <hr class="line">
  
−
<div style="padding-left:170px;padding-right:170px;color:white">
+
<div style="padding-left:200px;padding-right:200px;color:black">
 
<h1>Preparation</h1>
 
<h1>Preparation</h1>
−
                        <h2>What Is Interlab?</h2>
 
 
<p>
 
<p>
 
<font size="5">
 
<font size="5">
−
This is the 5th year of InterLab and we have the following question:
+
We participated in answering the following question during the InterLab study of this year:
 
</font>
 
</font>
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<b>
+
<br/>
 +
                        <b>
 
<font color = "orange" size = "4" face = "serif">
 
<font color = "orange" size = "4" face = "serif">
−
Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?  
+
    Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?  
 
</font>
 
</font>
 
</b>
 
</b>
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</div>
 
</div>
 
 
−
<div style="padding-left:170px;padding-right:170px;color:white">
+
<div style="padding-left:200px;padding-right:200px;color:black">
 
        <h2>Materials</h2>
 
        <h2>Materials</h2>
−
<p>DNA/Plasmids</p>
+
<p><font size="4">DNA/Plasmids</p>
 
<ol>
 
<ol>
 
<li>Negative Control: BBa_R0040</li>
 
<li>Negative Control: BBa_R0040</li>
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<li>Test Device 6: BBa_J364009</li>
 
<li>Test Device 6: BBa_J364009</li>
 
</ol>
 
</ol>
−
<p>Apparatus</p >
+
<p><font size="4">Apparatus</font></p >
 
<ul>
 
<ul>
 
<li>96 well plates (provided by Peking University)</li>
 
<li>96 well plates (provided by Peking University)</li>
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<li>Pipettes</li>
 
<li>Pipettes</li>
 
</ul>
 
</ul>
−
<p>Materials</p>
+
<p><font size="4">Materials</font></p>
 
<ul>
 
<ul>
 
<li>LUDOX CL-X</li>
 
<li>LUDOX CL-X</li>
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</div>
 
</div>
 
 
−
<div style="padding-left:170px;padding-right:170px;color:white">
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<div style="padding-left:200px;padding-right:200px;color:black">
 
<h2>Protocols</h2>
 
<h2>Protocols</h2>
 
<div>
 
<div>
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                 <div>
 
                 <div>
−
<h1>Experiment Process</h1>
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−
<p></p>
+
−
<div>
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−
<p></p>
+
−
<img src = "" alt = "Open Distribution Kit" style = "width: 30%">
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−
</div>
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−
<div>
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−
<p></p>
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−
<img src = "" alt = "Add silica beads to 96 well plate" style = "width: 30%">
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−
</div>
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<div>
 
<div>
 
<p></p>
 
<p></p>
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<div>
 
<div>
 
<h1>Results</h1>
 
<h1>Results</h1>
−
<p><i>The results are shown in the tables and figures.</i></p>
 
 
<h2>I.Calibrations</h2>
 
<h2>I.Calibrations</h2>
−
<div class="inlabdiv">
+
<div>
−
<p>OD<sub>600</sub> reference point:</p>
+
<p><font size = "3"><b>OD<sub>600</sub> reference point:</b></font></p>
−
<img src="https://static.igem.org/mediawiki/2018/a/af/T--BJRS_China--OD600_Reference_Point.png" alt = "OD600 Reference Point" style = "width: 30%"><br>
+
<figure class="text-center">
−
<font size = "1">Table 1. OD600 Reference Point.</font>
+
                                <img src="https://static.igem.org/mediawiki/2018/a/af/T--BJRS_China--OD600_Reference_Point.png" alt = "OD<sub>600</sub> Reference Point" style = "width: 50%">
 +
                                <figcaption><font size = "1">
 +
                                    Table 1. OD600 Reference Point.</font>
 +
                                </figcaption>
 +
                        </figure>
 +
<p></p>
 
</div>
 
</div>
  
−
<div class="inlabdiv">
+
<div>
−
                                 <p>Particle Standard Curve</p>
+
                                 <p><font size = "3"><b>Particle Standard Curve</b></font></p>
−
<img src = "https://static.igem.org/mediawiki/2018/e/ef/T--BJRS_China--Particle_Standard_Curve.jpg" alt = "Particle Standard Curve" style = "width: 50%"><br>
+
        <figure class="text-center">
−
<font size = "1">Figure 1. Particle Standard Curve.</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/e/ef/T--BJRS_China--Particle_Standard_Curve.jpg" alt = "Particle Standard Curve" style = "width: 70%">
 +
                                <figcaption><font size = "1">
 +
                                    Figure 1. Particle Standard Curve.</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
−
<div class="inlabdiv">
+
<div>
−
                                 <p>Particle Standard Curve(log scale)</p>
+
                                 <p><font size = "3"><b>Particle Standard Curve(log scale)</b></font></p>
−
<img src = "https://static.igem.org/mediawiki/2018/d/dd/T--BJRS_China--Particle_Standard_Curve%28log_scale%29.jpg" alt = "Particle Standard Curve(log scale)" style = "width: 50%"><br>
+
<figure class="text-center">
−
<font size = "1">Figure 1. Particle Standard Curve(log scale).</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/d/dd/T--BJRS_China--Particle_Standard_Curve%28log_scale%29.jpg" alt = "Particle Standard Curve(log scale)" style = "width: 70%">
 +
                                <figcaption><font size = "1">
 +
                                    Figure 1. Particle Standard Curve(log scale).</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
 
                          
 
                          
−
                         <div class="inlabdiv">
+
                         <div>
−
                                 <p>Fluorescein Standard Curve</p>
+
                                 <p><font size = "3"><b>Fluorescein Standard Curve</b></font></p>
−
<img src = "https://static.igem.org/mediawiki/2018/9/95/T--BJRS_China--Fluorescein_Standard_Curve.png" alt = "Fluorescein Standard Curve" style = "width: 50%"><br>
+
<figure class="text-center">
−
<font size = "1">Figure 2. Fluorescein Standard Curve.</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/9/95/T--BJRS_China--Fluorescein_Standard_Curve.png" alt = "Fluorescein Standard Curve" style = "width: 70%">
 +
                                <figcaption><font size = "1">
 +
                                    Figure 2. Fluorescein Standard Curve.</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
−
                         <div class="inlabdiv">
+
                         <div>
−
                                 <p>Fluorescein Standard Curve(log scale)</p>
+
                                 <p><font size = "3"><b>Fluorescein Standard Curve(log scale)</b></font></p>
−
<img src = "https://static.igem.org/mediawiki/2018/9/97/T--BJRS_China--Fluorescein_Standard_Curve%28log_scale%29.png" alt = "Fluorescein Standard Curve(log scale)" style = "width: 50%"><br>
+
        <figure class="text-center">
−
<font size = "1">Figure 2. Fluorescein Standard Curve(log scale).</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/9/97/T--BJRS_China--Fluorescein_Standard_Curve%28log_scale%29.png" alt = "Fluorescein Standard Curve(log scale)" style = "width: 70%">
 +
                                <figcaption><font size = "1">
 +
                                    Figure 2. Fluorescein Standard Curve(log scale).</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
 
<h2>II.Cell Measurements</h2>
 
<h2>II.Cell Measurements</h2>
−
<div class="inlabdiv">
+
<div>
−
<p><b>Fluorescence Raw</b></p>
+
<p><font size = "3"><b>Fluorescence Raw</b></font></p>
−
<img src = "https://static.igem.org/mediawiki/2018/0/06/T--BJRS_China--Fluorescence_Raw_0_hour.jpg" alt = "Fluorescence at 0h" style = "width: 70%"><br>
+
<figure class="text-center">
−
<font size = "1">Table 2. Raw Plate Reader Measurements of Fluorescence Raw at 0 hour.</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/0/06/T--BJRS_China--Fluorescence_Raw_0_hour.jpg" alt = "Fluorescence at 0h" style = "width: 80%">
 +
                                <figcaption><font size = "1">
 +
                                    Table 2. Raw Plate Reader Measurements of Fluorescence Raw at 0 hour.</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
−
<div class = "inlabdiv">
+
<div>
−
<img src = "https://static.igem.org/mediawiki/2018/1/1d/T--BJRS_China--Fluorescence_Raw_6_hour.jpg" alt = "Fluorescence at 6h" style = "width: 70%"><br>
+
<figure class="text-center">
−
<font size = "1">Table 3. Raw Plate Reader Measurements of Fluorescence Raw at 6 hours.</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/1/1d/T--BJRS_China--Fluorescence_Raw_6_hour.jpg" alt = "Fluorescence at 6h" style = "width: 80%">
 +
                                <figcaption><font size = "1">
 +
                                    Table 3. Raw Plate Reader Measurements of Fluorescence Raw at 6 hours.</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
−
<div class = "inlabdiv">
+
<div>
−
<p><b>Abs<sub>600</sub> Raw</b></p>
+
<p><font size = "3"><b>Abs<sub>600</sub> Raw</b></font></p>
−
<img src = "https://static.igem.org/mediawiki/2018/5/59/T--BJRS_China--Abs600_Raw_0_hour.jpg" alt = "Abs600 at 0h" style = "width: 70%"><br>
+
<figure class="text-center">
−
<font size = "1">Table 4. Raw Plate Reader Measurements of Abs600 Raw at 0 hour.</font>
+
                                <img src = "https://static.igem.org/mediawiki/2018/5/59/T--BJRS_China--Abs600_Raw_0_hour.jpg" alt = "Abs600 at 0h" style = "width: 80%">
 +
                                <figcaption><font size = "1">
 +
                                    Table 4. Raw Plate Reader Measurements of Abs600 Raw at 0 hour.</font>
 +
                                </figcaption>
 +
                        </figure>
 
</div>
 
</div>
−
<div class = "inlabdiv">
+
<div>
−
<img src = "https://static.igem.org/mediawiki/2018/8/86/T--BJRS_China--Abs600_Raw_6_hour.jpg" alt = "Abs600 at 6h" style = "width: 70%"><br>
+
<figure class="text-center">
−
<font size = "1">Table 5. Raw Plate Reader Measurements of Abs600 Raw at 6 hours.</font>
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                                <img src = "https://static.igem.org/mediawiki/2018/8/86/T--BJRS_China--Abs600_Raw_6_hour.jpg" alt = "Abs600 at 6h" style = "width: 80%">
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                                    Table 5. Raw Plate Reader Measurements of Abs600 Raw at 6 hours.</font>
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<h1>Discussion</h1>
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<h1>Our Thoughts and Feelings</h1>
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Latest revision as of 23:54, 17 October 2018

BJRS

InterLab

Preparation

We participated in answering the following question during the InterLab study of this year:
Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?

Materials

DNA/Plasmids

  1. Negative Control: BBa_R0040
  2. Positive Control: BBa_I20270
  3. Test Device 1: BBa_J364000
  4. Test Device 2: BBa_J364001
  5. Test Device 3: BBa_J364002
  6. Test Device 4: BBa_J364007
  7. Test Device 5: BBa_J364008
  8. Test Device 6: BBa_J364009

Apparatus

  • 96 well plates (provided by Peking University)
  • Plate reader
  • Foil covered 50 ml tube
  • Eppendorf tubes
  • Pipettes

Materials

  • LUDOX CL-X
  • Silica beads
  • Fluorescein
  • Phosphate buffered saline
  • LB media
  • Chloramphenicol
  • LB plates
  • distilled water

Protocols

We followed the protocol provided by iGEM HQ so that inter-laboratory errors can be reduced. Protocols we used can be found here:

2018 InterLab Plate Reader Protocol
Help: Protocols/Transformation

Results

I.Calibrations

OD600 reference point:

OD<sub>600</sub> Reference Point
Table 1. OD600 Reference Point.

Particle Standard Curve

Particle Standard Curve
Figure 1. Particle Standard Curve.

Particle Standard Curve(log scale)

Particle Standard Curve(log scale)
Figure 1. Particle Standard Curve(log scale).

Fluorescein Standard Curve

Fluorescein Standard Curve
Figure 2. Fluorescein Standard Curve.

Fluorescein Standard Curve(log scale)

Fluorescein Standard Curve(log scale)
Figure 2. Fluorescein Standard Curve(log scale).

II.Cell Measurements

Fluorescence Raw

Fluorescence at 0h
Table 2. Raw Plate Reader Measurements of Fluorescence Raw at 0 hour.
Fluorescence at 6h
Table 3. Raw Plate Reader Measurements of Fluorescence Raw at 6 hours.

Abs600 Raw

Abs600 at 0h
Table 4. Raw Plate Reader Measurements of Abs600 Raw at 0 hour.
Abs600 at 6h
Table 5. Raw Plate Reader Measurements of Abs600 Raw at 6 hours.