Difference between revisions of "File:T--SMS Shenzhen--Shiyan1.png"

 
 
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{{SMS_Shenzhen/CSS}}
  
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<img src="https://static.igem.org/mediawiki/2018/b/b6/T--SMS_Shenzhen--15.jpg" width="100%" >
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            <img src="https://static.igem.org/mediawiki/2018/8/8a/T--SMS_Shenzhen--ResultTop.jpg" width="100%" >
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        <h1>Experiment Data</h1>
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<img src="https://static.igem.org/mediawiki/2018/c/cc/T--SMS_Shenzhen--Shiyan1.png" >
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<h1>Analysis</h1>
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  <p style="font-size:18px"> The experiment 1 illustrates the relationship between the concentration of glucose and the absorbance of the solution with DNS colorant. By using linear regression, we constructed a function with glucose concentration (μmol) as the x-axis and the ab-sorbance as the y-axis. </p>
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<p style="font-size:18px">In the experiment 2, we measured the stability of sucrose and dextran, as well as pH stability. We let the reaction take place in the hot pot set at 100℃. We observed that dextran has a poor hot stability. Moreover, the sucrose will decompose spontaneous-ly under the acidic condition while heat doesn’t affect the decomposition of sucrose.
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          <h1> Analysis</h1> 
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  <p style="font-size:18px"> From the first half of the graph, we can clearly discover the linear relationship be-tween enzyme concentration and the absorbance. Data suggest that as the concentra-tion of enzyme becomes greater, the relative absorbance increase proportionately. This result justified our assumption. However, the latter half illustrated a negative-related relationship. We suspected that we didn’t use enough substrate to allow the decomposition reaction. Also, we assumed that excessive concentration of enzymes will negatively affected the reaction rate.</p>
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<img src="https://static.igem.org/mediawiki/2018/5/55/T--SMS_Shenzhen--green.jpg" width="100%" >

Latest revision as of 01:13, 18 October 2018

Title

Title

Experiment Data

Analysis

The experiment 1 illustrates the relationship between the concentration of glucose and the absorbance of the solution with DNS colorant. By using linear regression, we constructed a function with glucose concentration (μmol) as the x-axis and the ab-sorbance as the y-axis.

In the experiment 2, we measured the stability of sucrose and dextran, as well as pH stability. We let the reaction take place in the hot pot set at 100℃. We observed that dextran has a poor hot stability. Moreover, the sucrose will decompose spontaneous-ly under the acidic condition while heat doesn’t affect the decomposition of sucrose.

Analysis

From the first half of the graph, we can clearly discover the linear relationship be-tween enzyme concentration and the absorbance. Data suggest that as the concentra-tion of enzyme becomes greater, the relative absorbance increase proportionately. This result justified our assumption. However, the latter half illustrated a negative-related relationship. We suspected that we didn’t use enough substrate to allow the decomposition reaction. Also, we assumed that excessive concentration of enzymes will negatively affected the reaction rate.

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Horizontal resolution 47.24 dpc
Vertical resolution 47.24 dpc