Difference between revisions of "Team:NEFU China/Improve"

 
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Line 1: Line 1:
 
<html lang="en">
 
<html lang="en">
−
 
 
<head>
 
<head>
 
<meta charset="utf-8">
 
<meta charset="utf-8">
−
<meta http-equiv="X-UA-Compatible" content="IE=edge">
+
 
−
<meta name="viewport" content="width=device-width, initial-scale=1">
+
<title>Improvement Parts</title>
−
<title>Improved Parts</title>
+
−
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-members-foot&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
+
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
−
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-style-basicparts&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
+
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-style-improveparts&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 +
 
 
<style>
 
<style>
−
 
 
#top_title, #sideMenu{
 
#top_title, #sideMenu{
 
     display: none;
 
     display: none;
Line 22: Line 19:
 
}
 
}
 
#foot-title h3 {
 
#foot-title h3 {
−
font-size:18px!important;
+
    color:rgba(165,165,165,1.00);
 +
    line-height: 20px;
 +
    font-size: 18px;
 +
}
 +
#menu li ul li:hover ul{
 +
    background:rgba(0,0,0,0.75)!important;
 +
}
 +
li#mainlevel_01 a {
 +
    color: #FFE5B5!important;
 +
 +
}
 +
li#mainlevel_01 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_02 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_02 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_03 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_03 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_05 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_05 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_06 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_06 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_07 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_07 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_08 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_08 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
#menu li ul li ul li a:hover {
 +
    color: rgba(0,223,252,1);
 +
    border-top: dotted 1px rgba(255,255,255,0.91);
 +
    border-bottom: dotted 1px rgba(255,255,255,0.91);
 +
    background: rgba(0,223,252,.02);
 +
}
 +
#nav .mainlevel a {
 +
    color: black;
 +
    text-decoration:none;
 +
    line-height:32px;
 +
    display:block;
 +
    padding:0 5px;
 +
    font-size: 25px!important;
 +
font-family: 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif, 'Apple Color Emoji', 'Segoe UI Emoji', 'Segoe UI Symbol', 'Noto Color Emoji' !important;
 +
}
 +
.layer-bottom {
 +
z-index: -2;
 +
position: absolute;
 +
margin-top: 36px!important;
 +
}
 +
.basicpart-content {
 +
    padding-left: 150px;
 +
    padding-right: 150px;
 +
    padding-top: 40px;
 +
}
 +
 
 +
table.table {
 +
    background-color: transparent;
 +
}
 +
 
 +
.table th {
 +
    font-size: 22px;
 +
}
 +
 
 +
.table tbody {
 +
    background-color: transparent!important;
 +
}
 +
 
 +
.table td {
 +
    background-color: transparent;
 +
    font-size: 20px;
 +
    vertical-align: middle!important;
 +
    line-height: 27px;
 +
}
 +
 
 +
table.table {
 +
    background-color: rgb(0,0,0,0.6)!important;
 +
    color: white;
 +
}
 +
#background-reference {
 +
    padding-top: 50px;
 +
    padding-left: 150px;
 +
    padding-right:150px;
 +
    border:2px;
 +
    border-style: none;
 +
    font-family:"Open Sans" !important;
 +
    font-size:15px;
 +
    color: white !important;
 +
    box-shadow:inset 0px 15px 15px -15px #000;
 +
}
 +
 
 +
div#background-reference {}
 +
#background-reference h1 {
 +
    font-size: 50px;
 +
    color: white!important;
 +
}
 +
#background-reference p{
 +
    padding-top: 20px;
 +
    text-align:justify;
 +
    font-size: 20px;
 +
    text-justify:inter-ideograph;
 +
    color: white!important;
 +
}
 +
#background-reference a {
 +
    text-decoration: none;
 +
    color: white;
 +
}
 +
#background-reference a:hover{
 +
    color: orange;
 
}
 
}
 
</style>
 
</style>
−
</head>
 
  
 +
</head>
 
<body>
 
<body>
 
<!--menu-->
 
<!--menu-->
−
<div id="menu">
+
 
−
<li id="nav">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
+
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
−
<a class="menu1" href="#" style="text-align: right;">☰</a>
+
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 +
 
<ul class="firstmenu" style="float: left">
 
<ul class="firstmenu" style="float: left">
 
 
Line 42: Line 196:
 
<ul id="sub_02">
 
<ul id="sub_02">
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION &amp; DESIGN</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Design" target="_self">DESIGN</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODE BOOK</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODING BOOK</a></li>
+
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
<li class="mainlevel" id="mainlevel_03">
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic_Part"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENTS</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">INFORMATION DESTRUCTION</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">Pre-RNA SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
      <li class="mainlevel" id="mainlevel_04">
+
   
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
+
−
  <ul id="sub_04">
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
+
−
  </ul>
+
−
  </li>
+
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <ul id="sub_05">
 
  <ul id="sub_05">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">MODEL1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">MODEL2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">KILLING MODEL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 76: Line 230:
 
  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 85: Line 240:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 91: Line 245:
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <ul id="sub_08">
 
  <ul id="sub_08">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Human_Practices" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 108: Line 256:
 
</li>
 
</li>
 
</div>
 
</div>
−
<div id="lead">
+
<div class="layer-bottom">
−
    <div id="lead-content">
+
−
        <h1>Improved Parts</h1>
+
<canvas id="canvas" style="background:#000000"></canvas>
−
        <h2>Improved Parts's introduction.</h2>
+
 
−
    </div>
+
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 2700;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 
 +
 
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
context.fillStyle = "rgba(0,0,0,0.2)";
 +
context.fillRect(0,0,W,H);
 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
for(var i=0;i<colunms/2;i++){
 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 
 +
</script>
 +
 +
           
 +
 
</div>
 
</div>
−
 
+
<header id="home-banner">
−
<div id="about" style="box-shadow: inset 0px 15px 15px -15px green;">
+
    <div class="row banner">
−
<div class="container">
+
        <div class="banner-text">
−
<div class="row">
+
         
−
                <div class="col-md-4">
+
              
−
                    <h2 class="heading">Improved Parts</h2>
+
         </div>
−
                </div>
+
−
                <div class="col-md-8">
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                    <p>
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                        IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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                    </p>
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        </div>
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<hr style="text-align: center;margin-left: 150px;margin-right: 150px;">
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<div class="humanpractice">
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    <h2 class="heading">Improved Parts</h2>
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        <h3>title：111111111111111</h3>
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        <p>
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             IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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        </p>
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    </div>
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    <div id="title2" class="humanpractice-block">
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<h3>title：222222222222222</h3>
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<p>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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         </p>
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    </div>
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<div id="title3" class="humanpractice-block">
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<h3>title：333333333333333</h3>
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<p>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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        </p>
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    </div>
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<div id="title4" class="humanpractice-block">
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<h3>title：444444444444444</h3>
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<p>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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        </p>
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    </div>
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<div id="title5" class="humanpractice-block">
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<h3>title：555555555555555</h3>
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<p>
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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        </p>
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     </div>
 
     </div>
 +
</header>
 +
<div class="basicpart-content">
 +
<h1 style="font-size: 65px;color: orange;height: 84px;">Improvement parts</h1>
 +
<table class="table">
 +
    <thead>
 +
<tr>
 +
  <th style="width: 185px;">Number</th>
 +
<th style="width: 185px;">Name</th>
 +
<th style="">Description</th>
 +
    </tr>
 +
    </thead>
 +
    <tbody>
 +
<tr>
 +
<td>        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542014" style="color:orange;"> BBa_K2542014</a></td>
 +
<td><a href="#" style="color: yellow">pFUS1-EGFP-tCYC</a></td>
 +
 +
<td><p style="
 +
    font-size: 26px!important;
 +
">
 +
Our team were aimed at adjusting the expression level of any existing promoter. Thus, we modified the Kozak sequence of the promoter in the plasmid <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K775004" style="color:orange;">BBa_K775004</a>, a part from the University of the TU-Delft 2012 iGEM team. Our goal is to reduce the expression efficacy of this vector, and make it suitable to express a suicide gene for information destruction. To reduce the expression efficiency of this part in yeast, we modified the Kozak sequence by mutating the sequence “AAAAAA” to “GCCACC”. After that, our information destruction system was built and kill the information host yeast in a desired period of time. See more details in the basic part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2542014" style="color:orange;">BBa_K2542014</a>. We used the enhanced green fluorescent protein (EGFP) to test the effect of this modification. The&nbsp;generated plasmid&nbsp;allows&nbsp;a&nbsp;type&nbsp;yeast&nbsp;to&nbsp;sense&nbsp;the&nbsp; factor&nbsp;secreted&nbsp;by&nbsp;α-type&nbsp;yeast&nbsp;and&nbsp;express&nbsp;EGFP. Thus, when the promoter was activated by &nbsp;factor&nbsp;secreted&nbsp;by&nbsp;α-type&nbsp;yeast, the EGFP would be expressed. Thus, the EGFP expression would be the standard to tell the strength of our improved promoter.</p></td>
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">
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We creat a new parts BBa_K2542014，which is based on BBa_K775004. BBa_K2542014 is more suitable for somewhere which needs low expression in yeast.It's allows a type yeast to sense the α factor secreted by α-type yeast and express green fluorescent protein. We modified the part BBa_K775004 of the previous team by turning mutating the sequence “AAAAAA” into “GCCACC” to make the expression efficiency weaker，and make it more appropriate for suicide gene（Information Destroyed ). We constructed two plasmids with different kozak sequences and transferred them into yeast. Then, we use SD-Trp medium to culture yeast, and add with the solution which containing α factor. The solution containing α factor is obtain by extracting the liquid supernatant of the culture medium for culturing α-type yeast. After we add 200ul the α factor sloution to each one and culture yeast for 20 hours，we measured the expression of GFP in yeast by flow cytometry.
 +
</p>
 
<br>
 
<br>
 
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<div align="center">
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    <img src="https://static.igem.org/mediawiki/2018/c/c3/T--NEFU_China--KozakResult.jpg">
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<div id="foot-title">
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<h1>Userfull links</h1>
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<a href="https://2018.igem.org/Team:NEFU_China">Home</a>
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&nbsp;&nbsp;&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Model">Model</a>
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                        &nbsp;&nbsp;
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                        <a href="https://2018.igem.org/Team:NEFU_China/Description">Project</a>
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&nbsp;&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Software">Software</a>
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    &nbsp;&nbsp;
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<h2>
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<a href="https://2018.igem.org/Team:NEFU_China/Basic_Part">Parts</a>
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&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Members">Teams</a>
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&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Lock_Key">Results</a>
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&nbsp;
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<a href="https://2018.igem.org/Team:NEFU_China/Notebook">Notebook</a>
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                        &nbsp;&nbsp;
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</h2>
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<h2>
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<a href="https://2018.igem.org/Team:NEFU_China/Human_Practices">Human &nbsp;Practice</a>
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<td style="padding-left: 70px">
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<h1>Follow us</h1>
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<img alt="facebook" src="https://static.igem.org/mediawiki/2018/b/b5/T--NEFU_China--facebook.png">
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&nbsp;&nbsp;&nbsp;
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        <img alt="twitter" src="https://static.igem.org/mediawiki/2018/3/36/T--NEFU_China--twitter.png">
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&nbsp;&nbsp;&nbsp;
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<img alt="wehchat" src="https://static.igem.org/mediawiki/2018/c/ca/T--NEFU_China--wechat.png">
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        <img alt="facebook" src="https://static.igem.org/mediawiki/2018/b/b5/T--NEFU_China--facebook.png">
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&nbsp;&nbsp;&nbsp;
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        <img alt="twitter" src="https://static.igem.org/mediawiki/2018/3/36/T--NEFU_China--twitter.png">
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&nbsp;&nbsp;&nbsp;
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<img alt="wehchat" src="https://static.igem.org/mediawiki/2018/c/ca/T--NEFU_China--wechat.png">
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<td style="padding-left: 70px">
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<h1>Contact us</h1>
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<h3>iGEM-NEFU_China2018</h3>
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<h3>Email: hexinglu@nefu.edu.cn</h3>
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<h3>No.26 Hexing Road, Xiangfang <br>District, Harbin, Heilongjiang <br>Province 150000</h3>
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Latest revision as of 01:47, 18 October 2018

Improvement Parts

Improvement parts

Number Name Description
BBa_K2542014 pFUS1-EGFP-tCYC

Our team were aimed at adjusting the expression level of any existing promoter. Thus, we modified the Kozak sequence of the promoter in the plasmid BBa_K775004, a part from the University of the TU-Delft 2012 iGEM team. Our goal is to reduce the expression efficacy of this vector, and make it suitable to express a suicide gene for information destruction. To reduce the expression efficiency of this part in yeast, we modified the Kozak sequence by mutating the sequence “AAAAAA” to “GCCACC”. After that, our information destruction system was built and kill the information host yeast in a desired period of time. See more details in the basic part BBa_K2542014. We used the enhanced green fluorescent protein (EGFP) to test the effect of this modification. The generated plasmid allows a type yeast to sense the  factor secreted by α-type yeast and express EGFP. Thus, when the promoter was activated by  factor secreted by α-type yeast, the EGFP would be expressed. Thus, the EGFP expression would be the standard to tell the strength of our improved promoter.

We creat a new parts BBa_K2542014，which is based on BBa_K775004. BBa_K2542014 is more suitable for somewhere which needs low expression in yeast.It's allows a type yeast to sense the α factor secreted by α-type yeast and express green fluorescent protein. We modified the part BBa_K775004 of the previous team by turning mutating the sequence “AAAAAA” into “GCCACC” to make the expression efficiency weaker，and make it more appropriate for suicide gene（Information Destroyed ). We constructed two plasmids with different kozak sequences and transferred them into yeast. Then, we use SD-Trp medium to culture yeast, and add with the solution which containing α factor. The solution containing α factor is obtain by extracting the liquid supernatant of the culture medium for culturing α-type yeast. After we add 200ul the α factor sloution to each one and culture yeast for 20 hours，we measured the expression of GFP in yeast by flow cytometry.