Difference between revisions of "Team:SMS Shenzhen/Results"

 
(4 intermediate revisions by 3 users not shown)
Line 11: Line 11:
  
 
<body>
 
<body>
 +
 +
 
<div id="sec2">
 
<div id="sec2">
 
     <div id="page">
 
     <div id="page">
−
            <img src="https://static.igem.org/mediawiki/2018/8/8a/T--SMS_Shenzhen--ResultTop.jpg" width="100%" >
+
<h1>I Glucose Standard Curve</h1>
−
        <h1>Experiment Data</h1>
+
<center>
−
    <b style="font-size: 20px"></b>
+
    <img src="https://static.igem.org/mediawiki/2018/5/51/T--SMS_Shenzhen--%E8%91%A1%E8%90%84%E7%B3%96%E6%A0%87%E5%87%86%E6%9B%B2%E7%BA%BF.PNG"width="80%" >
−
    </div>
+
<h1> </h1>
−
</div>
+
  
−
<div id="sec1">
+
<img src="https://static.igem.org/mediawiki/2018/c/cc/T--SMS_Shenzhen--Shiyan1.png" width="80%">
−
    <div id="page">
+
<h1> </h1>
−
    <img src="https://static.igem.org/mediawiki/2018/5/51/T--SMS_Shenzhen--%E8%91%A1%E8%90%84%E7%B3%96%E6%A0%87%E5%87%86%E6%9B%B2%E7%BA%BF.PNG" >
+
<img src="https://static.igem.org/mediawiki/2018/8/8a/T--SMS_Shenzhen--ResultTop.jpg" width="80%" >
−
<img src="https://static.igem.org/mediawiki/2018/c/cc/T--SMS_Shenzhen--Shiyan1.png" >
+
</center>
  
−
<h1>Analysis</h1>
+
<h1> </h1>
 +
<h2>Data Analysis:</h2>
 
   <p style="font-size:18px"> The experiment 1 illustrates the relationship between the concentration of glucose and the absorbance of the solution with DNS colorant. By using linear regression, we constructed a function with glucose concentration (μmol) as the x-axis and the ab-sorbance as the y-axis. </p>
 
   <p style="font-size:18px"> The experiment 1 illustrates the relationship between the concentration of glucose and the absorbance of the solution with DNS colorant. By using linear regression, we constructed a function with glucose concentration (μmol) as the x-axis and the ab-sorbance as the y-axis. </p>
  
Line 33: Line 35:
 
</div>
 
</div>
  
−
<div id="sec2">
+
<div id="sec3">
 
     <div id="page">
 
     <div id="page">
−
    <img src="https://static.igem.org/mediawiki/2018/0/02/T--SMS_Shenzhen--%E9%85%B6%E6%B4%BB%E5%9B%BE%E7%89%87.PNG" >
+
<h1>II DEX Enzyme Activity </h1>
 +
<h1> </h1>
  
−
          <h1> Analysis</h1>   
+
<center>
−
   <p style="font-size:18px"> From the first half of the graph, we can clearly discover the linear relationship be-tween enzyme concentration and the absorbance. Data suggest that as the concentra-tion of enzyme becomes greater, the relative absorbance increase proportionately. This result justified our assumption. However, the latter half illustrated a negative-related relationship. We suspected that we didn’t use enough substrate to allow the decomposition reaction. Also, we assumed that excessive concentration of enzymes will negatively affected the reaction rate.</p>
+
    <img src="https://static.igem.org/mediawiki/2018/0/02/T--SMS_Shenzhen--%E9%85%B6%E6%B4%BB%E5%9B%BE%E7%89%87.PNG" width="100%">
 +
</center>
 +
<h1> </h1>
 +
          <h2> Data Analysis:</h2>   
 +
   <p style="font-size:18px"> From the first half of the graph, we can clearly discover the linear relationship between enzyme concentration and the absorbance. Data suggest that as the concentration of enzyme becomes greater, the relative absorbance increase proportionately. This result justified our assumption. However, the latter half illustrated a negative-related relationship. We suspected that we didn’t use enough substrate to allow the decomposition reaction. Also, we assumed that excessive concentration of enzymes will negatively affect the reaction rate.</p>
  
−
    </div>
 
−
</div>
 
−
 
−
<div id="sec1">
 
−
    <div id="page">
 
−
           
 
 
     </div>
 
     </div>
 
</div>
 
</div>

Latest revision as of 02:21, 18 October 2018

Title

Title

I Glucose Standard Curve

Data Analysis:

The experiment 1 illustrates the relationship between the concentration of glucose and the absorbance of the solution with DNS colorant. By using linear regression, we constructed a function with glucose concentration (μmol) as the x-axis and the ab-sorbance as the y-axis.

In the experiment 2, we measured the stability of sucrose and dextran, as well as pH stability. We let the reaction take place in the hot pot set at 100℃. We observed that dextran has a poor hot stability. Moreover, the sucrose will decompose spontaneous-ly under the acidic condition while heat doesn’t affect the decomposition of sucrose.

II DEX Enzyme Activity

Data Analysis:

From the first half of the graph, we can clearly discover the linear relationship between enzyme concentration and the absorbance. Data suggest that as the concentration of enzyme becomes greater, the relative absorbance increase proportionately. This result justified our assumption. However, the latter half illustrated a negative-related relationship. We suspected that we didn’t use enough substrate to allow the decomposition reaction. Also, we assumed that excessive concentration of enzymes will negatively affect the reaction rate.