Difference between revisions of "Team:Calgary/HomeImposter"

 
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 +
{{:Team:Calgary/Layout}}
 
<html lang="en">
 
<html lang="en">
−
    <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/WikiResetCSSImposter?action=raw&ctype=text/css">
 
−
    <head>
 
−
        <meta charset="utf-8">
 
−
        <title>Team:Calgary - 2018.igem.org</title>
 
−
        <meta name="viewport" content="width=device-width, initial-scale=1">
 
  
−
        <!-- Styles -->
+
<head>
 +
    <title>Team:Calgary - 2018.igem.org</title>
 +
    <!-- CSS -->
 +
    <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/jquery_fullPage_css?action=raw&ctype=text/css">
 +
    <link rel="stylesheet" href="https://use.fontawesome.com/releases/v5.2.0/css/all.css" integrity="sha384-hWVjflwFxL6sNzntih27bfxkr27PmbbK/iSvJ+a4+0owXq79v+lsFkW54bOGbiDQ"
 +
        crossorigin="anonymous">
 +
    <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/animation_css?action=raw&ctype=text/css">
 +
    <style>
 +
        .maincontent {
 +
            margin-top: 180px;
 +
            margin-bottom: 180px;
 +
        }
  
−
         <!-- Bootstrap 4's CSS -->
+
         h1 {
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/BootstrapMinCSS?action=raw&ctype=text/css">
+
            font-family: 'Josefin Sans', sans-serif !important;
−
        <!-- Long Scroll CSS -->
+
            font-weight: normal !important;
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/JQueryFullPageCSS?action=raw&ctype=text/css">
+
            text-align: center;
−
         <!-- Font Awesome CSS -->
+
         }
  
−
         <!-- UH OH! CDN! -->
+
         h2 {
 +
            text-align: center;
 +
            line-height: 1.8;
 +
        }
  
−
         <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css">
+
         p {
 +
            font-size: 20px !important;
 +
            line-height: 2;
 +
        }
  
−
         <!-- Home Page CSS -->
+
         .info-link {
−
         <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/HomePageCSSImposter?action=raw&ctype=text/css">
+
            padding: 0 !important;
 +
         }
  
−
         <!-- Scripts -->
+
         a:link {
 +
            color: #f38f8f;
 +
        }
  
−
         <!-- Long Scroll JS -->
+
         a:visited {
−
         <script src="https://2018.igem.org/Team:Calgary/JQueryMinJSImposter?action=raw&ctype=text/javascript"></script>
+
            color: #f38f8f;
−
        <script src="https://2018.igem.org/Team:Calgary/JQueryFullPageJSImposter?action=raw&ctype=text/javascript"></script>
+
        }
−
        <script>
+
 
−
            $(document).ready(function() {
+
        a:hover {
−
                $('#fullpage').fullpage({
+
            color: #f38f8f;
−
                    anchors: ['Project', 'Software', 'Human Practices', 'Contact Us'],
+
        }
−
                    sectionsColor: ['#F53240', '#F9BE02', '#02C8A7', '#AF7AC5'],
+
 
−
                    navigation: true,
+
        a:active {
−
                    navigationPosition: 'right',
+
            color: #f38f8f;
−
                    navigationTooltips: ['Project','Software','Human Practices', 'Contact Us'],
+
        }
−
                    css3: true,
+
 
−
                    responsiveWidth: 750,
+
        #moving-helix {
−
                    responsiveHeight: 400,
+
            height: 400px;
−
                    loopTop: true,
+
        }
−
                    loopBottom: true,
+
 
−
                    afterResponsive: function(isResponsive){}
+
        #blinking-arrows {
−
                });
+
            height: 54px;
 +
        }
 +
 
 +
        .bottom-left {
 +
            bottom: 20px;
 +
            left: 20px;
 +
            line-height: 3;
 +
            position: fixed;
 +
            text-align: center;
 +
         }
 +
 
 +
        .bottom-right {
 +
            bottom: 20px;
 +
            display: inline-block;
 +
            line-height: 1.5;
 +
            position: fixed;
 +
            right: 70px;
 +
            text-align: center;
 +
        }
 +
 
 +
        .social-media-icon {
 +
            color: rgba(0, 0, 0, 0.5);
 +
            font-size: 24px;
 +
        }
 +
 
 +
        .social-media-icon:hover {
 +
            color: rgba(0, 0, 0, 0.9);
 +
        }
 +
 
 +
        .vertical-align {
 +
            display: flex;
 +
            justify-content: center;
 +
            align-items: center;
 +
        }
 +
 
 +
        .home-title {
 +
            color: #44414d;
 +
            font-size: 80px;
 +
        }
 +
 
 +
        #home-subtitle {
 +
            color: #44414d;
 +
            font-size: 47px;
 +
        }
 +
 
 +
        #social-media-icons {
 +
            list-style: none;
 +
        }
 +
 
 +
        #instructions {
 +
            display: block;
 +
            font-size: 12px;
 +
        }
 +
 
 +
        .wrapper {
 +
            text-align: center;
 +
        }
 +
 
 +
        .maincontent {
 +
            background-color: #eeedf0;
 +
            margin-top: 210px;
 +
            margin-bottom: 210px;
 +
            padding: 0;
 +
        }
 +
 
 +
        .sef>p {
 +
            text-align: center !important;
 +
        }
 +
 
 +
        @media only screen and (max-width: 1050px) {
 +
            .bottom-left {
 +
                display: none;
 +
            }
 +
 
 +
            .bottom-right {
 +
                display: none;
 +
            }
 +
        }
 +
 
 +
        @media only screen and (max-width: 400px) {
 +
            h2 {
 +
                font-size: 18px;
 +
            }
 +
 
 +
            .home-title {
 +
                color: #44414d;
 +
                font-size: 26px;
 +
            }
 +
 
 +
            #home-subtitle {
 +
                color: #44414d;
 +
                font-size: 16px;
 +
            }
 +
 
 +
            .fp-tableCell {
 +
                display: block;
 +
                height: 100% !important;
 +
                padding-bottom: 0;
 +
                padding-top: 0;
 +
            }
 +
 
 +
            .fp-table {
 +
                height: 100% !important;
 +
            }
 +
        }
 +
    </style>
 +
    <!-- JS -->
 +
    <script src="https://2018.igem.org/Team:Calgary/jquery_fullPage_js?action=raw&ctype=text/javascript"></script>
 +
    <script>
 +
        $(document).ready(function () {
 +
            $('#fullpage').fullpage({
 +
                loopBottom: true,
 +
                loopTop: true,
 +
                responsiveWidth: 1050,
 +
                afterResponsive: function (isResponsive) { }
 
             });
 
             });
−
         </script>
+
         });
−
        <!-- Bootstrap 4's JS -->
+
    </script>
−
        <script src="https://2018.igem.org/Team:Calgary/BootstrapBundleMinJSImposter?action=raw&ctype=text/javascript"></script>
+
</head>
  
−
    </head>
+
<body>
−
     <body>
+
     <!-- Content -->
−
        <!-- Navigation Bar -->
+
    <div class="container" id="fullpage">
−
        <div id="header">
+
        <!-- Description -->
−
            <nav class="navbar navbar-expand-md bg-light navbar-light">
+
        <div class="section">
−
                <a class="navbar-brand" href="#">
+
            <div class="row vertical-align">
−
                    <img src="https://static.igem.org/mediawiki/2018/9/91/T--Calgary--FLogo.png" alt="F Logo" class="logo">
+
                 <div class="col-10">
−
                </a>
+
                     <h1 class="home-title">SNIP
−
                <button class="navbar-toggler" type="button"data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
+
                         <span style="color: #7ccfb8">EQUIP</span> FLIP</h1>
−
                    <span class="navbar-toggler-icon"></span>
+
−
                </button>
+
−
                 <div class="collapse navbar-collapse" id="navbarSupportedContent">
+
−
                     <ul class="navbar-nav mr-auto">
+
−
                         <li class="nav-item active">
+
−
                            <a class="nav-link" href="#">Home</a>
+
−
                        </li>
+
−
                        <li class="nav-item active dropdown">
+
−
                            <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
+
−
                                Project
+
−
                            </a>
+
−
                            <div id="drop" class="dropdown-menu" aria-labelledby="navbarDropdown">
+
−
                                <a class="dropdown-item" href="#">Problem</a>
+
−
                                <a class="dropdown-item" href="#">Description</a>
+
−
                                <a class="dropdown-item" href="#">Synthesis</a>
+
−
                            </div>
+
−
                        </li>
+
−
                        <li class="nav-item active ">
+
−
                            <a class="nav-link" href="#">Notebook</a>
+
−
                        </li>
+
−
                        <li class="nav-item active">
+
−
                            <a class="nav-link" href="#">Team</a>
+
−
                        </li>
+
−
                        <li class="nav-item active">
+
−
                            <a class="nav-link" href="#">Human Practices</a>
+
−
                        </li>
+
−
                        <li class="nav-item active">
+
−
                            <a class="nav-link" href="#">Judging</a>
+
−
                        </li>
+
−
                    </ul>
+
 
                 </div>
 
                 </div>
−
            </nav>
+
                <div class="col-2">
−
        </div>
+
                    <svg style="width: 50%" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:cc="http://creativecommons.org/ns#"
−
        <!-- Content -->
+
                        xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:svg="http://www.w3.org/2000/svg"
−
        <div id="fullpage">
+
                        xmlns="http://www.w3.org/2000/svg" xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd"
−
            <!-- Project Preview -->
+
                        xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape" viewBox="0 0 79.80928 292.63452"
−
            <div class="section" id="section0">
+
                        version="1.1" id="moving-helix" inkscape:version="0.92.3 (2405546, 2018-03-11)"
−
                <div class="intro" id="slide0">
+
                        sodipodi:docname="Helix.svg">
−
                    <h1>Project</h1>
+
                        <defs id="defs1848" />
−
                    <p>Blah, blah, blah, this is our project. Yikes.</p>
+
                        <sodipodi:namedview id="base" pagecolor="#ffffff" bordercolor="#666666" borderopacity="1.0"
 +
                            inkscape:pageopacity="0.0" inkscape:pageshadow="2" inkscape:zoom="0.64" inkscape:cx="40.612564"
 +
                            inkscape:cy="524.45313" inkscape:document-units="mm" inkscape:current-layer="layer1"
 +
                            showgrid="false" inkscape:window-width="1920" inkscape:window-height="1017"
 +
                            inkscape:window-x="-8" inkscape:window-y="32" inkscape:window-maximized="1" borderlayer="false"
 +
                            fit-margin-top="0" fit-margin-left="0" fit-margin-right="0" fit-margin-bottom="0"
 +
                            showborder="false" />
 +
                        <metadata id="metadata1851">
 +
                            <rdf:RDF>
 +
                                <cc:Work rdf:about="">
 +
                                    <dc:format>image/svg+xml</dc:format>
 +
                                    <dc:type rdf:resource="http://purl.org/dc/dcmitype/StillImage" />
 +
                                    <dc:title></dc:title>
 +
                                </cc:Work>
 +
                            </rdf:RDF>
 +
                        </metadata>
 +
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                 </div>
 
                 </div>
 
             </div>
 
             </div>
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            <!-- Software Preview -->
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        </div>
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            <div class="section" id="section1">
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        <!-- Page 2 -->
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                <div class="intro" id="slide1">
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        <div class="section">
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                    <h1>Software</h1>
+
            <div class="section-content">
−
                    <p>We made a bot. It does some cool stuff.</p>
+
                <h2 style="text-align: left; color: #44414d">The ideal medicine is not a perfect treatment . . .</h2>
−
                </div>
+
                <br>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: right; color: #44414d">. . . it's a <span style="color: #7ccfb8">cure</span></h2>
 
             </div>
 
             </div>
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            <!-- Human Practices Preview -->
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        </div>
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            <div class="section" id="section2">
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        <!-- Page 3 -->
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                <div class="intro" id="slide2">
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        <div class="section">
−
                     <h1>Human Practices</h1>
+
            <div class="section-content">
−
                    <p>Humans were involved in the creation of this project.</p>
+
                <h2 style="text-align: left; color: #44414d">Advancements in genetic modification have opened avenues
−
                 </div>
+
                     towards numerous tracks of scientific
 +
                    development.</h2>
 +
                <br>
 +
                <br>
 +
                <br>
 +
                 <h2 style="text-align: left; color: #44414d">In particular,
 +
                    the demand for permanent health solutions has sparked massive interest in <span style="color: #7ccfb8">gene
 +
                        therapy</span>.</h2>
 
             </div>
 
             </div>
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            <!-- Social Media -->
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        </div>
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            <div class="section" id="section3">
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        <!-- Page 4 -->
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                <div class="intro" id="slide3">
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        <div class="section">
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                     <h1>Contact Us</h1>
+
            <div class="section-content">
−
                    <ul id="social-media-list">
+
                <h2 style="text-align: left; color: #44414d">Despite the potential for establishing an ideal medicine,
−
                        <li><a href="#"><i class="fa fa-facebook-f fa-2x" aria-hidden="true"></i><span>Facebook</span></a></li>
+
                     there are still numerous challenges.</h2>
−
                        <li><a href="#"><i class="fa fa-twitter fa-2x" aria-hidden="true"></i><span>Twitter</span></a></li>
+
                <br>
−
                        <li><a href="#"><i class="fa fa-instagram fa-2x" aria-hidden="true"></i><span>Instagram</span></a></li>
+
                <br>
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                        <li><a href="#"><i class="fa fa-envelope fa-2x" aria-hidden="true"></i><span>Email</span></a></li>
+
                <br>
−
                     </ul>
+
                <h2 style="text-align: left; color: #44414d">The selection of an <span style="color: #7ccfb8">integration
−
                </div>
+
                        target site</span>,
 +
                    methodology pertaining to <span style="color: #7ccfb8">gene
 +
                        delivery</span>, and <span style="color: #7ccfb8">maintenance</span> of gene expression
 +
                     currently limit the potential for gene
 +
                    therapy
 +
                    to become common practice.</h2>
 
             </div>
 
             </div>
 
         </div>
 
         </div>
−
     </body>
+
        <!-- Page 5 -->
 +
        <div class="section">
 +
            <div class="section-content">
 +
                <h2 style="text-align: center; color: #44414d">How do we overcome these problems?</h2>
 +
                <br>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: center; color: #44414d" class="home-title">SNIP
 +
                    <span style="color: #7ccfb8">EQUIP</span> FLIP</h2>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: center; color: #44414d">The system that allows for the integration and
 +
                    maintenance of large-scale genetic constructs in eukaryotic systems.</h2>
 +
            </div>
 +
        </div>
 +
        <!-- Page 6 -->
 +
        <div class="section">
 +
            <h2 style="text-align: left; color: #44414d">University of Calgary’s Snip, Equip, Flip aims to overcome
 +
                the aforementioned obstacles. This system
 +
                allows for the creation of eukaryotic cell lines via stable integration and expression of exogenous
 +
                genes.</h2>
 +
            <br>
 +
            <br>
 +
            <br>
 +
            <h2 style="text-align: left; color: #44414d">Snip, Equip, Flip is a simple and reliable method that can
 +
                be used by experienced and entrant
 +
                researchers alike that can lead to a vast array of scientific discoveries.</h2>
 +
        </div>
 +
        <!-- Page 7 -->
 +
        <div class="section">
 +
            <h2 style="text-align: left; color: #44414d">By complementing the specificity of targeting that
 +
                CRISPR/Cas9 offers with the much larger integration
 +
                capabilities of FLP recombinase and beta resolvase, gene integration into eukaryotic genomes becomes a
 +
                simple process of Snip, Equip, Flip.</h2>
 +
            <br>
 +
            <br>
 +
            <br>
 +
            <h2 style="text-align: left; color: #44414d">The expression of integrated genes is then protected and
 +
                maintained over time by adding flanking
 +
                chromatin modifying elements.</h2>
 +
            <br>
 +
            <div style="text-align: center">
 +
                <a href="https://2018.igem.org/Team:Calgary/Description"><button style="font-size: 20px" type="button"
 +
                        class="btn btn-outline-dark btn-lg">Our
 +
                        System</button></a>
 +
            </div>
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        </div>
 +
     </div>
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Latest revision as of 03:01, 18 October 2018

Team:Calgary - 2018.igem.org

SNIP EQUIP FLIP

image/svg+xml

The ideal medicine is not a perfect treatment . . .




. . . it's a cure

Advancements in genetic modification have opened avenues towards numerous tracks of scientific development.




In particular, the demand for permanent health solutions has sparked massive interest in gene therapy.

Despite the potential for establishing an ideal medicine, there are still numerous challenges.




The selection of an integration target site, methodology pertaining to gene delivery, and maintenance of gene expression currently limit the potential for gene therapy to become common practice.

How do we overcome these problems?




SNIP EQUIP FLIP



The system that allows for the integration and maintenance of large-scale genetic constructs in eukaryotic systems.

University of Calgary’s Snip, Equip, Flip aims to overcome the aforementioned obstacles. This system allows for the creation of eukaryotic cell lines via stable integration and expression of exogenous genes.




Snip, Equip, Flip is a simple and reliable method that can be used by experienced and entrant researchers alike that can lead to a vast array of scientific discoveries.

By complementing the specificity of targeting that CRISPR/Cas9 offers with the much larger integration capabilities of FLP recombinase and beta resolvase, gene integration into eukaryotic genomes becomes a simple process of Snip, Equip, Flip.




The expression of integrated genes is then protected and maintained over time by adding flanking chromatin modifying elements.


image/svg+xml Use arrow keys
or scroll