Difference between revisions of "Team:Calgary/HomeImposter"

 
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{{:Team:Calgary/NavBar}}
+
{{:Team:Calgary/Layout}}
−
 
+
 
<html lang="en">
 
<html lang="en">
−
    <head>
 
−
        <meta charset="utf-8">
 
−
        <title>Team:Calgary - 2018.igem.org</title>
 
−
        <meta name="viewport" content="width=device-width, initial-scale=1">
 
  
−
        <!-- Styles -->
+
<head>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/WikiResetCSSImposter?action=raw&ctype=text/css">
+
    <title>Team:Calgary - 2018.igem.org</title>
−
        <!-- Bootstrap 4's CSS -->
+
    <!-- CSS -->
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/BootstrapMinCSS?action=raw&ctype=text/css">
+
    <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/jquery_fullPage_css?action=raw&ctype=text/css">
−
         <!-- Long Scroll CSS -->
+
    <link rel="stylesheet" href="https://use.fontawesome.com/releases/v5.2.0/css/all.css" integrity="sha384-hWVjflwFxL6sNzntih27bfxkr27PmbbK/iSvJ+a4+0owXq79v+lsFkW54bOGbiDQ"
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/JQueryFullPageCSS?action=raw&ctype=text/css">
+
         crossorigin="anonymous">
−
        <!-- Font Awesome CSS -->
+
    <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/animation_css?action=raw&ctype=text/css">
 +
    <style>
 +
        .maincontent {
 +
            margin-top: 180px;
 +
            margin-bottom: 180px;
 +
        }
  
−
         <!-- UH OH! CDN! -->
+
         h1 {
 +
            font-family: 'Josefin Sans', sans-serif !important;
 +
            font-weight: normal !important;
 +
            text-align: center;
 +
        }
  
−
         <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css">
+
         h2 {
 +
            text-align: center;
 +
            line-height: 1.8;
 +
        }
  
−
         <!-- Home Page CSS -->
+
         p {
−
         <link rel="stylesheet" href="https://2018.igem.org/Team:Calgary/HomePageCSSImposter?action=raw&ctype=text/css">
+
            font-size: 20px !important;
 +
            line-height: 2;
 +
         }
  
−
         <!-- Scripts -->
+
         .info-link {
 +
            padding: 0 !important;
 +
        }
  
−
         <!-- Long Scroll JS -->
+
         a:link {
−
         <script src="https://2018.igem.org/Team:Calgary/JQueryMinJSImposter?action=raw&ctype=text/javascript"></script>
+
            color: #f38f8f;
−
        <script src="https://2018.igem.org/Team:Calgary/JQueryFullPageJSImposter?action=raw&ctype=text/javascript"></script>
+
        }
−
        <script>
+
 
−
            $(document).ready(function() {
+
        a:visited {
−
                $('#fullpage').fullpage({
+
            color: #f38f8f;
−
                    anchors: ['Project', 'Software', 'Human Practices', 'Contact Us'],
+
        }
−
                    sectionsColor: ['#F53240', '#F9BE02', '#02C8A7', '#AF7AC5'],
+
 
−
                    navigation: true,
+
        a:hover {
−
                    navigationPosition: 'right',
+
            color: #f38f8f;
−
                    navigationTooltips: ['Project','Software','Human Practices', 'Contact Us'],
+
        }
−
                    css3: true,
+
 
−
                    responsiveWidth: 750,
+
        a:active {
−
                    responsiveHeight: 400,
+
            color: #f38f8f;
−
                    loopTop: true,
+
        }
−
                    loopBottom: true,
+
 
−
                    afterResponsive: function(isResponsive) {}
+
        #moving-helix {
−
                });
+
            height: 400px;
 +
        }
 +
 
 +
        #blinking-arrows {
 +
            height: 54px;
 +
        }
 +
 
 +
        .bottom-left {
 +
            bottom: 20px;
 +
            left: 20px;
 +
            line-height: 3;
 +
            position: fixed;
 +
            text-align: center;
 +
         }
 +
 
 +
        .bottom-right {
 +
            bottom: 20px;
 +
            display: inline-block;
 +
            line-height: 1.5;
 +
            position: fixed;
 +
            right: 70px;
 +
            text-align: center;
 +
        }
 +
 
 +
        .social-media-icon {
 +
            color: rgba(0, 0, 0, 0.5);
 +
            font-size: 24px;
 +
        }
 +
 
 +
        .social-media-icon:hover {
 +
            color: rgba(0, 0, 0, 0.9);
 +
        }
 +
 
 +
        .vertical-align {
 +
            display: flex;
 +
            justify-content: center;
 +
            align-items: center;
 +
        }
 +
 
 +
        .home-title {
 +
            color: #44414d;
 +
            font-size: 80px;
 +
        }
 +
 
 +
        #home-subtitle {
 +
            color: #44414d;
 +
            font-size: 47px;
 +
        }
 +
 
 +
        #social-media-icons {
 +
            list-style: none;
 +
        }
 +
 
 +
        #instructions {
 +
            display: block;
 +
            font-size: 12px;
 +
        }
 +
 
 +
        .wrapper {
 +
            text-align: center;
 +
        }
 +
 
 +
        .maincontent {
 +
            background-color: #eeedf0;
 +
            margin-top: 210px;
 +
            margin-bottom: 210px;
 +
            padding: 0;
 +
        }
 +
 
 +
        .sef>p {
 +
            text-align: center !important;
 +
        }
 +
 
 +
        @media only screen and (max-width: 1050px) {
 +
            .bottom-left {
 +
                display: none;
 +
            }
 +
 
 +
            .bottom-right {
 +
                display: none;
 +
            }
 +
        }
 +
 
 +
        @media only screen and (max-width: 400px) {
 +
            h2 {
 +
                font-size: 18px;
 +
            }
 +
 
 +
            .home-title {
 +
                color: #44414d;
 +
                font-size: 26px;
 +
            }
 +
 
 +
            #home-subtitle {
 +
                color: #44414d;
 +
                font-size: 16px;
 +
            }
 +
 
 +
            .fp-tableCell {
 +
                display: block;
 +
                height: 100% !important;
 +
                padding-bottom: 0;
 +
                padding-top: 0;
 +
            }
 +
 
 +
            .fp-table {
 +
                height: 100% !important;
 +
            }
 +
        }
 +
    </style>
 +
    <!-- JS -->
 +
    <script src="https://2018.igem.org/Team:Calgary/jquery_fullPage_js?action=raw&ctype=text/javascript"></script>
 +
    <script>
 +
        $(document).ready(function () {
 +
            $('#fullpage').fullpage({
 +
                loopBottom: true,
 +
                loopTop: true,
 +
                responsiveWidth: 1050,
 +
                afterResponsive: function (isResponsive) { }
 
             });
 
             });
−
         </script>
+
         });
−
        <!-- Bootstrap 4's JS -->
+
    </script>
−
        <script src="https://2018.igem.org/Team:Calgary/BootstrapBundleMinJSImposter?action=raw&ctype=text/javascript"></script>
+
</head>
  
−
    </head>
+
<body>
−
     <body>
+
     <!-- Content -->
−
 
+
    <div class="container" id="fullpage">
−
        <!-- Content -->
+
        <!-- Description -->
−
        <div id="fullpage">
+
        <div class="section">
−
            <!-- Project Preview -->
+
            <div class="row vertical-align">
−
            <div class="section" id="section0">
+
                 <div class="col-10">
−
                 <div class="intro" id="slide0">
+
                    <h1 class="home-title">SNIP
−
                    <h1>Project</h1>
+
                        <span style="color: #7ccfb8">EQUIP</span> FLIP</h1>
−
                    <p>Blah, blah, blah, this is our project. Yikes.</p>
+
 
                 </div>
 
                 </div>
−
            </div>
+
                <div class="col-2">
−
            <!-- Software Preview -->
+
                    <svg style="width: 50%" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:cc="http://creativecommons.org/ns#"
−
            <div class="section" id="section1">
+
                        xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:svg="http://www.w3.org/2000/svg"
−
                <div class="intro" id="slide1">
+
                        xmlns="http://www.w3.org/2000/svg" xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd"
−
                    <h1>Software</h1>
+
                        xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape" viewBox="0 0 79.80928 292.63452"
−
                    <p>We made a bot. It does some cool stuff.</p>
+
                        version="1.1" id="moving-helix" inkscape:version="0.92.3 (2405546, 2018-03-11)"
 +
                        sodipodi:docname="Helix.svg">
 +
                        <defs id="defs1848" />
 +
                        <sodipodi:namedview id="base" pagecolor="#ffffff" bordercolor="#666666" borderopacity="1.0"
 +
                            inkscape:pageopacity="0.0" inkscape:pageshadow="2" inkscape:zoom="0.64" inkscape:cx="40.612564"
 +
                            inkscape:cy="524.45313" inkscape:document-units="mm" inkscape:current-layer="layer1"
 +
                            showgrid="false" inkscape:window-width="1920" inkscape:window-height="1017"
 +
                            inkscape:window-x="-8" inkscape:window-y="32" inkscape:window-maximized="1" borderlayer="false"
 +
                            fit-margin-top="0" fit-margin-left="0" fit-margin-right="0" fit-margin-bottom="0"
 +
                            showborder="false" />
 +
                        <metadata id="metadata1851">
 +
                            <rdf:RDF>
 +
                                <cc:Work rdf:about="">
 +
                                    <dc:format>image/svg+xml</dc:format>
 +
                                    <dc:type rdf:resource="http://purl.org/dc/dcmitype/StillImage" />
 +
                                    <dc:title></dc:title>
 +
                                </cc:Work>
 +
                            </rdf:RDF>
 +
                        </metadata>
 +
                        <g inkscape:label="Layer 1" inkscape:groupmode="layer" id="layer1" transform="translate(-2.4802186,-2.7247285)">
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                            <g class="move-up" id="g2473" transform="matrix(1.3829225,0,0,1.3829225,-103.49477,-58.214956)">
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                                <path class="dark-grey appear-right-delay" id="dgrey4" sodipodi:nodetypes="ccccc"
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                                    inkscape:connector-curvature="0" d="m 134.34178,5.592011 v 19.23694 l 57.7106,38.473729 V 44.065821 Z" />
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                                <path class="dark-green move-right appear-right" id="dgreen3" sodipodi:nodetypes="ccccc"
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                                    inkscape:connector-curvature="0" d="m 134.34175,44.065952 v 19.236859 l 57.7106,38.473789 V 82.539731 Z" />
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                                <path class="light-grey disappear-right-delay" id="lgrey2" sodipodi:nodetypes="ccccc"
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                        </g>
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                 </div>
 
                 </div>
 
             </div>
 
             </div>
−
            <!-- Human Practices Preview -->
+
        </div>
−
            <div class="section" id="section2">
+
        <!-- Page 2 -->
−
                <div class="intro" id="slide2">
+
        <div class="section">
−
                    <h1>Human Practices</h1>
+
            <div class="section-content">
−
                    <p>Humans were involved in the creation of this project.</p>
+
                <h2 style="text-align: left; color: #44414d">The ideal medicine is not a perfect treatment . . .</h2>
−
                </div>
+
                <br>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: right; color: #44414d">. . . it's a <span style="color: #7ccfb8">cure</span></h2>
 
             </div>
 
             </div>
−
            <!-- Social Media -->
+
        </div>
−
            <div class="section" id="section3">
+
        <!-- Page 3 -->
−
                <div class="intro" id="slide3">
+
        <div class="section">
−
                    <h1>Contact Us</h1>
+
            <div class="section-content">
−
                    <ul id="social-media-list">
+
                <h2 style="text-align: left; color: #44414d">Advancements in genetic modification have opened avenues
−
                        <li><a href="#"><i class="fa fa-facebook-f fa-2x" aria-hidden="true"></i><span>Facebook</span></a></li>
+
                    towards numerous tracks of scientific
−
                        <li><a href="#"><i class="fa fa-twitter fa-2x" aria-hidden="true"></i><span>Twitter</span></a></li>
+
                    development.</h2>
−
                        <li><a href="#"><i class="fa fa-instagram fa-2x" aria-hidden="true"></i><span>Instagram</span></a></li>
+
                <br>
−
                         <li><a href="#"><i class="fa fa-envelope fa-2x" aria-hidden="true"></i><span>Email</span></a></li>
+
                <br>
−
                    </ul>
+
                <br>
−
                </div>
+
                <h2 style="text-align: left; color: #44414d">In particular,
 +
                    the demand for permanent health solutions has sparked massive interest in <span style="color: #7ccfb8">gene
 +
                         therapy</span>.</h2>
 
             </div>
 
             </div>
 
         </div>
 
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     </body>
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        <!-- Page 4 -->
 +
        <div class="section">
 +
            <div class="section-content">
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                <h2 style="text-align: left; color: #44414d">Despite the potential for establishing an ideal medicine,
 +
                    there are still numerous challenges.</h2>
 +
                <br>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: left; color: #44414d">The selection of an <span style="color: #7ccfb8">integration
 +
                        target site</span>,
 +
                    methodology pertaining to <span style="color: #7ccfb8">gene
 +
                        delivery</span>, and <span style="color: #7ccfb8">maintenance</span> of gene expression
 +
                    currently limit the potential for gene
 +
                    therapy
 +
                    to become common practice.</h2>
 +
            </div>
 +
        </div>
 +
        <!-- Page 5 -->
 +
        <div class="section">
 +
            <div class="section-content">
 +
                <h2 style="text-align: center; color: #44414d">How do we overcome these problems?</h2>
 +
                <br>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: center; color: #44414d" class="home-title">SNIP
 +
                    <span style="color: #7ccfb8">EQUIP</span> FLIP</h2>
 +
                <br>
 +
                <br>
 +
                <h2 style="text-align: center; color: #44414d">The system that allows for the integration and
 +
                    maintenance of large-scale genetic constructs in eukaryotic systems.</h2>
 +
            </div>
 +
        </div>
 +
        <!-- Page 6 -->
 +
        <div class="section">
 +
            <h2 style="text-align: left; color: #44414d">University of Calgary’s Snip, Equip, Flip aims to overcome
 +
                the aforementioned obstacles. This system
 +
                allows for the creation of eukaryotic cell lines via stable integration and expression of exogenous
 +
                genes.</h2>
 +
            <br>
 +
            <br>
 +
            <br>
 +
            <h2 style="text-align: left; color: #44414d">Snip, Equip, Flip is a simple and reliable method that can
 +
                be used by experienced and entrant
 +
                researchers alike that can lead to a vast array of scientific discoveries.</h2>
 +
        </div>
 +
        <!-- Page 7 -->
 +
        <div class="section">
 +
            <h2 style="text-align: left; color: #44414d">By complementing the specificity of targeting that
 +
                CRISPR/Cas9 offers with the much larger integration
 +
                capabilities of FLP recombinase and beta resolvase, gene integration into eukaryotic genomes becomes a
 +
                simple process of Snip, Equip, Flip.</h2>
 +
            <br>
 +
            <br>
 +
            <br>
 +
            <h2 style="text-align: left; color: #44414d">The expression of integrated genes is then protected and
 +
                maintained over time by adding flanking
 +
                chromatin modifying elements.</h2>
 +
            <br>
 +
            <div style="text-align: center">
 +
                <a href="https://2018.igem.org/Team:Calgary/Description"><button style="font-size: 20px" type="button"
 +
                        class="btn btn-outline-dark btn-lg">Our
 +
                        System</button></a>
 +
            </div>
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        </div>
 +
     </div>
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Latest revision as of 03:01, 18 October 2018

Team:Calgary - 2018.igem.org

SNIP EQUIP FLIP

image/svg+xml

The ideal medicine is not a perfect treatment . . .




. . . it's a cure

Advancements in genetic modification have opened avenues towards numerous tracks of scientific development.




In particular, the demand for permanent health solutions has sparked massive interest in gene therapy.

Despite the potential for establishing an ideal medicine, there are still numerous challenges.




The selection of an integration target site, methodology pertaining to gene delivery, and maintenance of gene expression currently limit the potential for gene therapy to become common practice.

How do we overcome these problems?




SNIP EQUIP FLIP



The system that allows for the integration and maintenance of large-scale genetic constructs in eukaryotic systems.

University of Calgary’s Snip, Equip, Flip aims to overcome the aforementioned obstacles. This system allows for the creation of eukaryotic cell lines via stable integration and expression of exogenous genes.




Snip, Equip, Flip is a simple and reliable method that can be used by experienced and entrant researchers alike that can lead to a vast array of scientific discoveries.

By complementing the specificity of targeting that CRISPR/Cas9 offers with the much larger integration capabilities of FLP recombinase and beta resolvase, gene integration into eukaryotic genomes becomes a simple process of Snip, Equip, Flip.




The expression of integrated genes is then protected and maintained over time by adding flanking chromatin modifying elements.


image/svg+xml Use arrow keys
or scroll