Difference between revisions of "Team:SMS Shenzhen/Parts"

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<h1>Parts</h1>
 
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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        <h1>Parts Design:</h1>
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        <h3>    </h3>
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        <h1>Expression Plasmid in E. coli:</h1>
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        <p style="font-size:18px">So as to test the effect of the two enzymes, dextranase and fructosidase, we constructed expression plasmids in E. coli to acquire the proteins and verify the enzyme activity under the experimental condition, stimulating the ph and temperature of human mouth. </p>
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        <img src=" https://static.igem.org/mediawiki/2018/a/a5/T--SMS_Shenzhen--Ds3.jpg  " width="80%" ></center>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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        <h1>Exogenous Nisin-induced Expression Vector:</h1>
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        <p style="font-size:18px">Strain Lactococcus lactisNZ3900 is a sensitive strain on the concentration of nisin because of its deficiency of immunity gene nisI. Expression of nisl in L. lactis provided the cells with a significant level of protection against exogenously added nisin, indicating that nisI plays a role in the immunity mechanism, also nisC. These genes instruct synthesize proteins could combine with nisin and recede its function as inducement. The original form of NZ3900 has the structural gene nisA, the precursor of Nisin. The gene of nisB helps to correctly fold and “process” the nisin precursor. So, it can produce nisin itself. There are two genes, nisR and nisK, serve to regulate the expression of nisA gene. Gene nisR works as a repressor, specifically bind a site after PnisA. When nisR binds this site, the transcription is locked. While gene nisK can phosphorylate nisR when nisin is specifically reacted with nisK. After the phosphorylation of nisR protein, the repressor is removed and the transcription of target gene can start.  So as to appropriately induce the expression of genes with promoter PnisA by exogenous Nisin, we intend to utilize CRISPR/Cas9 system to knock out nisA in the strain NZ3900. </p>
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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        <h1>Inducible Lysis-Promoting Elements:  </h1>
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        <p style="font-size:18px">In order to release the proteins synthesized inside the bacteria, a lysis system is needed in this program. iGEM parts BBa_K112000 (T4 holin) and BBa_K112806 (T4 endolysin) was integrated to “self- killing” plasmid, inducing by Nisin, to construct an element of promoting cell lysis, helping to release the intra expression protein (DEX and FruA) and ensure safety. </p>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h1>Successful Submission:</h1>
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        <img src=" https://static.igem.org/mediawiki/2018/6/64/T--SMS_Shenzhen--Parts.jpg " width="60%" >
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<img src="https://static.igem.org/mediawiki/2018/0/0f/T--SMS_Shenzhen--partttt.jpg" width="50%" >
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        <h3>    </h3>
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<img src="https://static.igem.org/mediawiki/2018/7/77/T--SMS_Shenzhen--parttt.jpg" width="50%" >
  
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 SMS_Shenzhen</groupparts>
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Latest revision as of 03:29, 18 October 2018

Title

Title

Parts Design:

Expression Plasmid in E. coli:

So as to test the effect of the two enzymes, dextranase and fructosidase, we constructed expression plasmids in E. coli to acquire the proteins and verify the enzyme activity under the experimental condition, stimulating the ph and temperature of human mouth.

Exogenous Nisin-induced Expression Vector:

Strain Lactococcus lactisNZ3900 is a sensitive strain on the concentration of nisin because of its deficiency of immunity gene nisI. Expression of nisl in L. lactis provided the cells with a significant level of protection against exogenously added nisin, indicating that nisI plays a role in the immunity mechanism, also nisC. These genes instruct synthesize proteins could combine with nisin and recede its function as inducement. The original form of NZ3900 has the structural gene nisA, the precursor of Nisin. The gene of nisB helps to correctly fold and “process” the nisin precursor. So, it can produce nisin itself. There are two genes, nisR and nisK, serve to regulate the expression of nisA gene. Gene nisR works as a repressor, specifically bind a site after PnisA. When nisR binds this site, the transcription is locked. While gene nisK can phosphorylate nisR when nisin is specifically reacted with nisK. After the phosphorylation of nisR protein, the repressor is removed and the transcription of target gene can start. So as to appropriately induce the expression of genes with promoter PnisA by exogenous Nisin, we intend to utilize CRISPR/Cas9 system to knock out nisA in the strain NZ3900.

Inducible Lysis-Promoting Elements:

In order to release the proteins synthesized inside the bacteria, a lysis system is needed in this program. iGEM parts BBa_K112000 (T4 holin) and BBa_K112806 (T4 endolysin) was integrated to “self- killing” plasmid, inducing by Nisin, to construct an element of promoting cell lysis, helping to release the intra expression protein (DEX and FruA) and ensure safety.

Successful Submission: