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− | {{Pasteur_Paris}} | + | {{Pasteur_Paris/MenuBlock}} |
− | <html>´ | + | {{Pasteur_Paris/Vignettes}} |
− | <head>
| + | <html> |
− | <style> | + | <style type="text/css"> |
− | .section_container{ /*element that contains a section */ | + | #runningchip { |
− | position:relative;
| + | left: 605px; |
− | margin-bottom:3em; | + | |
| } | | } |
| + | #labwork_small { |
| + | background-color: #292929; |
| + | } |
| + | #bannerchanged{ |
| + | width: 100%; |
| + | overflow: hidden; |
| + | margin: 0 auto; |
| + | } |
| + | @media screen and (max-width: 760px) { |
| + | /*#wrapper { |
| + | width: 100%; |
| + | overflow: hidden; |
| + | } |
| + | #container { |
| + | width: 100%; |
| + | margin: 0 auto; |
| + | }*/ |
| + | .banner-img { |
| + | width: 100%; |
| + | } |
| + | } |
| | | |
| + | </style> |
| | | |
− | .vignette { /*element that contains ONE vignette*/
| + | <div id="bannerchanged"> |
− | height:25em;
| + | <img class="banner-img" src="https://static.igem.org/mediawiki/2018/3/3e/T--Pasteur_Paris--Banner_Protocols.jpg"> |
− | width:25em;
| + | </div> |
− | margin:2em;
| + | <h1></h1> |
− | position:relative;
| + | </div> |
− | display:inline-block;
| + | |
− | } | + | |
| | | |
− |
| + | <div id="GeneralContent"> |
− | .vignette_for { /*foreground part of the vignettes, gets transparent*/ | + | <div id="MainContent"> |
− | width: 20em;
| + | |
− | height: 21em;
| + | |
− | position:absolute;
| + | |
− | left:2.5em;
| + | |
− | top:2em;
| + | |
| | | |
− | z-index: 2;
| + | <a href="https://2018.igem.org/Team:Pasteur_Paris/Protocols/CellBio"> |
− | border: 1px solid #333;
| + | <div class="block onglet"> |
− | border-radius:20px;
| + | <div class="ongletImg" style="background-image: url(https://static.igem.org/mediawiki/2018/c/c1/T--Pasteur_Paris--Logo-Big-Vignette-Cellular-Biology.png);"> |
− | background-image: url("https://static.igem.org/mediawiki/2018/c/cd/T--Pasteur_Paris--Logo-Neuronarch_gradient.jpg");
| + | </div> |
− | background-size:100%;
| + | <div class= "textOnglet" style="background-color: #dedede;"> |
| + | <h1>Microbiology</h1> |
| + | </div> |
| + | </div> |
| + | </a> |
| + | <a href="https://2018.igem.org/Team:Pasteur_Paris/Experiments/Microflu"> |
| + | <div class="block onglet"> |
| + | <div class="ongletImg" style="background-image: url(https://static.igem.org/mediawiki/2018/1/13/T--Pasteur_Paris--Logo-Big-Vignette-Microfluidics.png);"> |
| + | </div> |
| + | <div class= "textOnglet" style="background-color: #dedede;"> |
| + | <h1>Microfluidics</h1> |
| + | </div> |
| + | </div> |
| + | </a> |
| + | <a href="https://2018.igem.org/Team:Pasteur_Paris/Protocols/CellBio2"> |
| + | <div class="block onglet"> |
| + | <div class="ongletImg" style="background-image: url(https://static.igem.org/mediawiki/2018/3/3c/T--Pasteur_Paris--Logo-Big-Vignette-Molecular-Biology.png);"> |
| + | </div> |
| + | <div class= "textOnglet" style="background-color: #dedede;"> |
| + | <h1>Cellular Biology</h1> |
| + | </div> |
| + | </a> |
| + | </div> |
| | | |
− | pointer-events:none;
| |
| | | |
− | -webkit-transition: opacity 0.7s;
| + | <a href="https://2018.igem.org/Team:Pasteur_Paris/Technicals"> |
− | -moz-transition: opacity 0.7s;
| + | <div class="block onglet"> |
− | -o-transition: opacity 0.7s;
| + | <div class="ongletImg" style="background-image: url(https://static.igem.org/mediawiki/2018/e/ed/T--Pasteur_Paris--Big-Vignette-Design.png);"> |
− | transition: opacity 0.7s;
| + | </div> |
− | }
| + | <div class= "textOnglet" style="background-color: #dedede;"> |
| + | <h1>Design*</h1> |
| + | </div> |
| + | </a> |
| + | </div> |
| | | |
| | | |
− |
| + | <p> (*) There are not really protocols in design, but rather methodologies. You will be redirected to Product Design - Methodology. </p> |
− | .vignette_back { /*background part of the vignettes, picture*/
| + | |
− | width: 20em;
| + | |
− | height: 21em;
| + | |
− | position:absolute;
| + | |
− | left:2.5em;
| + | |
− | top:2em;
| + | |
| | | |
− | cursor:pointer;
| |
| | | |
− | z-index: 1;
| |
− | border: 1px solid #333;
| |
− | border-radius: 20px;
| |
− | background-image:url("https://static.igem.org/mediawiki/2018/0/03/T--Pasteur_Paris--PDMS_prep.jpg");
| |
− | background-size:100%;
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− | }
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− |
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− |
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− |
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− |
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− | .vignette_text { /*small panel that slides down on the vignettes*/
| |
− | width: 22em;
| |
− | height: 3em;
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− | position:absolute;
| |
− | top:5em;
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− | left:1.5em;
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− |
| |
− | z-index: 3;
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− | border: 1px solid #333;
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− | background-color:white;
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− | box-shadow: 2px 2px 3px grey;
| |
− | opacity: 0.8;
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− |
| |
− | pointer-events:none;
| |
− | -webkit-transition: top 0.7s, opacity 0.7s;
| |
− | -moz-transition: top 0.7s, opacity 0.7s;
| |
− | -o-transition: top 0.7s, opacity 0.7s;
| |
− | transition: top 0.7s, opacity 0.7s;
| |
− | }
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− |
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− |
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− | .panel {
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− | position:relative;
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− | top:25em;
| |
− | padding: 0px 18px;
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− | background-color: white;
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− | max-height: 0;
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− | overflow: hidden;
| |
− | -webkit-transition: max-height 0.7s;
| |
− | -moz-transition: max-height 0.7s ;
| |
− | -o-transition: max-height 0.7s ;
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− | transition: max-height 0.7s ;
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− | border: 10px solid transparent;
| |
− | -moz-border-image: -moz-linear-gradient(left, #C6234A 0%, #3C519F 100%);
| |
− | -webkit-border-image: -webkit-linear-gradient(left, #C6234A 0%, #3C519F 100%);
| |
− | border-image: linear-gradient(to right, #C6234A 0%, #3C519F 100%);
| |
− | border-image-slice: 1;
| |
− | }
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− |
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− |
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− |
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− | .close_button {
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− | width:3em;
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− | height:3em;
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− | position:absolute;
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− | right: 1em;
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− | top:1em;
| |
− | z-index:6;
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− |
| |
− | background-image: url("https://static.igem.org/mediawiki/2018/d/d3/T--Pasteur_Paris--Cross.png");
| |
− | background-size:100%;
| |
− | opacity:0.3;
| |
− | border-radius:4px;
| |
− |
| |
− | cursor:pointer;
| |
− | }
| |
− |
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− | .close_button:hover {
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− | opacity:1;
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− | }
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− |
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− |
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− | .close_button:active {
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− | background-color:#3C519F;
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− |
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− | }
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− |
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− |
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− | </style>
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− | </head>
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− |
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− | <body>
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− |
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− | <div class="column two_thirds_size">
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− | <h3>What should this page contain?</h3>
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− | <ul>
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− | <li> Protocols </li>
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− | <li> Experiments </li>
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− | <li> Documentation of the development of your project </li>
| |
− | </ul>
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− |
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− | </div>
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− |
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− | <div class="column third_size">
| |
− | <div class="highlight decoration_A_full">
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− | <h3>Inspiration</h3>
| |
− | <ul>
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− | <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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− | <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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− | <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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− | </ul>
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− | </div>
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− | </div>
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− |
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− |
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− | <div class="column full_size">
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− |
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− | <h1>Experiments</h1>
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− |
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− |
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− | <p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed
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− | enough for another team to repeat your experiments.</p>
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− |
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− | <p>
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− | Please remember to put all characterization and measurement data for your parts on the corresponding Registry
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− | part pages.
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− | </p>
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− |
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− | <div class="section_container" id="microfluidics_container">
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− | <h2 style="text-align: left;">Microfluidics</h2>
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− |
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− | <p>PDMS (Polydimethylsiloxane) is a widely used polymer in microfluidics, for its biocompatibility and transparence, among other qualities. Here we show how to prepare PDMS for
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− | microfluidic chips, as well as how to demold them, bond them to other surfaces and treat them for neuron growth.</p>
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− |
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− | <div class="vignette">
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− |
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− | <div class="vignette_for">
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− | </div>
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− |
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− | <div class="vignette_back">
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− | </div>
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− |
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− | <div class="vignette_text">
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− | <p style="margin:auto; text-align:center;font-weight:bold;">PDMS Preparation</p>
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− | </div>
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− |
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− | <div class="panel">
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− | <div class="close_button">
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− | </div>
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− |
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− | <h3>Materials</h3>
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− | <ul>
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− | <li> PDMS and curing agent (Sigma-aldrich, Sylgard 184, 761036-5EA) </li>
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− | <li> Isopropanol </li>
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− | </ul>
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− | <br>
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− | <h3>Protocol</h3>
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− | <p>According to <a href="https://static.igem.org/mediawiki/igem.org/2/29/T--Technion_Israel-HardwarespecsPDMS.pdf">manufacturer's instruction</a>. <br> Pour PDMS into a beaker (80 g should be
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− | enough for most applications). Pour curing agent into the same beaker (10 :1
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− | proportion : if you used 80 g PDMS, you need 8 g curing
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− | agent) and mix with a spatula for 30 seconds. Spatula can be cleaned afterwards with some paper dipped in isopropanol. Put beaker in vacuum machine in order to extract
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− | the
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− | air bubbles from the mixture (at least 10 minutes vacuum, look out for overflowings). Pour mixture onto mold. Put mold+mixture in oven at 70 degrees Celsius for 3 hours at
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− | least. We do not recommend letting the mold with the mixture more than 2 days in the oven, otherwise there is a chance you'll break the chip when demolding it.
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− | </p>
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− |
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− | </div>
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− | </div>
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− | <div class="vignette">
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− |
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− | <div class="vignette_for">
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− | </div>
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− |
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− | <div class="vignette_back">
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− | </div>
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− |
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− | <div class="vignette_text">
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− | <p style="margin:auto; text-align:center;font-weight:bold;">PDMS Preparation</p>
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− | </div>
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− |
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− |
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− | <div class="panel">
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− | <div class="close_button">
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− | </div>
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− |
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− | <h3>Materials</h3>
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− | <ul>
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− | <li> Bonjour </li>
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− | <li> Salut </li>
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− | </ul>
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− | <br>
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− | <h3>Protocol</h3>
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− | <p>J'en ai marre les amis</p>
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− |
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− | </div>
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| </div> | | </div> |
| </div> | | </div> |
− |
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− | <script>
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− |
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− | var acc = document.getElementsByClassName("vignette_back");
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− | var i;
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− |
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− | for (i = 0; i < acc.length; i++) {
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− | acc[i].addEventListener("click", function() {
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− | var panel = this.nextElementSibling.nextElementSibling;
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− | var vignFor = this.previousElementSibling;
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− | var vignText = this.nextElementSibling;
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− |
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− | if (panel.style.maxHeight){
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− | panel.style.maxHeight = null;
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− | vignFor.style.opacity = 1;
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− | vignText.style.opacity = 0.8;
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− | vignText.style.top = "5em";
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− | } else {
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− | panel.style.maxHeight = panel.scrollHeight + "px";
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− | vignFor.style.opacity = 0;
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− | vignText.style.opacity = 1;
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− | vignText.style.top = "20em";
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− | }
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− | });
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− | }
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− |
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− | var acc = document.getElementsByClassName("close_button");
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− |
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− | for (i = 0; i < acc.length; i++) {
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− | acc[i].addEventListener("click", function() {
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− | var panel = this.parentElement;
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− | var vignFor = this.parentElement.previousElementSibling.previousElementSibling.previousElementSibling;
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− | var vignText = this.parentElement.previousElementSibling;
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− | panel.style.maxHeight = null;
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− | vignFor.style.opacity = 1;
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− | vignText.style.opacity = 0.8;
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− | vignText.style.top = "5em";
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− | });
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− | }
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− | </script>
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− | <h2 style="text-align: left;">Cell culture and whatnot</h2>
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− | </div>
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− | <div class="clear"></div> | + | <footer> |
| + | </footer> |
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− | </body>
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| </html> | | </html> |