Difference between revisions of "Team:Jilin China"

 
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   <h1>The Collection of<br>Synthetic RNA-based Thermosensors<br>with Different Sensing Temperatures</h1>
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   <h1><span>The Collection of</span><br><span>SynRT</span><br><span>with Different Sensing Temperatures</span></h1>
 
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  <div>
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  <h1>ABSTRACT</h1>
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  <p>Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'-
 +
untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.</p>
 +
  <p>However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their application. To address this issue, we have created a synthetic RNA-based thermosensor (SynRT) toolkit with different sensing temperatures. Additionally, we built a matched search engine for users.
 +
  <p>The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:</p>
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   <p>Hot-induced 5'UTR</p>
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   <center><p><b>Heat-inducible RNA-based Thermosensor</b></p></center>
 
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  <center><p><b>Heat-repressible RNA-based Thermosensor</b></p></center>
  
 
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  <center><p><b>Cold-inducible RNA-based Thermosensor</b></p></center>
  
  
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  <center><p><b>Cold-repressible RNA-based Thermosensor</b></p></center>
  
  
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    <center><a href="https://2018.igem.org/Team:Jilin_China/Design">Learn More ></a></center>
 
   </div>
 
   </div>
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<div style="clear:both"></div>
 
  </section>
 
  </section>
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  <section class="ss3">
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  <section class="ss3 normal hp">
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  <h1>Human Practices</h1>
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  <div>
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    <p>Several Exploration Improved</p>
 +
    <p>OUR TOOLKITs</p>
 +
    <p>Meanwhile Inspiring Us to Make</p>
 +
    <p>A DECADE <br>WORKFLOW</p>
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  </div>
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  <img src="https://static.igem.org/mediawiki/2018/e/e1/T--Jilin_China--Home--HP--1.svg" />
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    <a href="https://2018.igem.org/Team:Jilin_China/Human_Practices">Hear Our Stories</a>
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  <br clear="all"/>
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  <div>
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    <p>Several Innovative Kits</p>
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    <p>Promoting Us to</p>
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    <p>SPREAD and DISCUSS</p>
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    <p>Synthetic Biology</p>
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  </div>
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  <img src="https://static.igem.org/mediawiki/2018/b/bc/T--Jilin_China--Home--HP--2.svg"/>
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  <a href="https://2018.igem.org/Team:Jilin_China/Public_Engagement">Hear Our Stories</a>
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  <br clear="all"/>
 
  </section>
 
  </section>
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  <section class="ss4">
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  <section class="ss4 normal model">
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  <h1>Model</h1><img src="https://static.igem.org/mediawiki/2018/b/b2/T--Jilin_China--Home_Model-curve.png" width="100%"></img>
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  <a href="https://2018.igem.org/Team:Jilin_China/Model">Learn More > </a>
 
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  </section>
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  <h1>More</h1>
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  <div>
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    <div>
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    <div class="ss5_background">
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    <a href="https://2018.igem.org/Team:Jilin_China/Background"><h3>Background</h3></a>
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    </div>
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    <div class="ss5_project">
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    <a href="https://2018.igem.org/Team:Jilin_China/Project/Overview"><h3>Project</h3></a>
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    </div>
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    </div>
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    <div>
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    <div class="ss5_result">
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    <a href="https://2018.igem.org/Team:Jilin_China/Result/Version_1"><h3>Results</h3></a>
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    </div>
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    <div class="ss5_engine">
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    <a href="https://2018.igem.org/Team:Jilin_China/Part/Search_Engine"><h3>Explorer</h3></a>
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    </div>
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    </div>
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    <div>
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    <div class="ss5_part">
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    <a href="https://2018.igem.org/Team:Jilin_China/Parts"><h3>Parts</h3></a>
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    </div>
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    <div class="ss5_team">
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    <a href="https://2018.igem.org/Team:Jilin_China/Team"><h3>Team</h3></a>
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    </div>
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    </div>
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  </div>
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</section>
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Latest revision as of 03:54, 18 October 2018

The Collection of
SynRT
with Different Sensing Temperatures

ABSTRACT

Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'- untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.

However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their application. To address this issue, we have created a synthetic RNA-based thermosensor (SynRT) toolkit with different sensing temperatures. Additionally, we built a matched search engine for users.

The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:

SD Sequence anti-SD Sequence Ribosome

Heat-inducible RNA-based Thermosensor

RC anti-RC RNAse E

Heat-repressible RNA-based Thermosensor

Cold-inducible RNA-based Thermosensor

Stroke Version_1 RC RNAse III

Cold-repressible RNA-based Thermosensor

Learn More >

Human Practices

Several Exploration Improved

OUR TOOLKITs

Meanwhile Inspiring Us to Make

A DECADE
WORKFLOW

Hear Our Stories

Several Innovative Kits

Promoting Us to

SPREAD and DISCUSS

Synthetic Biology

Hear Our Stories

Model

Learn More >

More