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| − | <h1> Project description Rheda_Bielefeld: </h1> | + | .title { |
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| | + | <div class="title"> INTERPOLLEN </div> <br/> |
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| | + | <img src="https://static.igem.org/mediawiki/2018/9/9c/T--Rheda_Bielefeld--HomeFlussDiagramm.png" style="float:left;padding-right:10px;"> |
| | + | <br><br><article style="font-size:24px;">We are the Highschool team Rheda_Bielefeld from Germany and our projects’ name is INTERPOLLEN. |
| | + | With our project we want to help as many people as possible and- because there are many people in Germany who are allergic to pollen and need to take a lot of medicine because of their allergy- we wanted to develop a test strip so that each individual person can measure the local pollen dispersal with it. |
| | + | In the flow chart on the left, you can see our workflow. |
| | + | <br>-A:<a href="https://2018.igem.org/Team:Rheda_Bielefeld/Pollen"style=color:yellow;"> we collected pollen to work with</a> |
| | + | <br>-B: <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Parts" style="color:yellow;"> we tried to integrate genes for pectinases into the E.coli so that it can produce pectinases to break the pollen </a> |
| | + | <br> -C: <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Pollen" style="color:yellow;">we extracted the pollens’ DNA</a> |
| | + | <br> -D+E:<a href="https://2018.igem.org/Team:Rheda_Bielefeld/Description" style="color:yellow;"> it is the idea of a test strip that identifies the pollen with our E.coli</a></article><br><br> |
| | + | <h2> Abstract </h2> |
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| − | <p>In the course of our research, our goal is to help people who are allergic to pollen. We are trying to differ pollen by their DNA sequence. So far we used the learned techniques for extracting DNA to get information about the genetic structure of pollen cores. By doing PCR and gel electrophoresis, we are planning to analyse the DNA of different species of pollen. In addition, we will use different techniques of microscopy to confirm our progress. With this gained information, we are going to be able to construct a sensor which can measure the concentration of pollen floating through the air. This will help allergic people pick the right dose of medicine before going outside. </p> | + | <article> A great percentage of Earth’s population is allergic to specific substances. Approximately 20 million people living in Germany are allergic to different plants, animals and much more, but close to half of them are allergic to pollen. We want to help those people suffering from an allergy to pollen by advising them which dose of medicine is necessary for each day. <br/><br/> |
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| | + | Although there is already useful medicine, we are convinced that we can optimize the use of such medicine and reduce the exposure to unnecessary drugs because they have negative side effects like lowering the personal performance capacity, causing the user to become tired and many others. <br/><br/> |
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| + | Therefore we use a DNA-based method which uses pectinase and cellulase to open the pollen and isolates their DNA. This DNA will be used for a PCR with specific primers against the birch allergen "Bet". By hereby identifying pollen we aim to measure the current pollen exposure in the air. </article> |
| − | <h1> Welcome to iGEM 2018! </h1>
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| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
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| − | <h3>Before you start</h3>
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| − | <p> Please read the following pages:</p>
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| − | <li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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| − | <li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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| − | <h3> Styling your wiki </h3>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <h3> Uploading pictures and files </h3>
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| − | <p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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| − | <p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | UPLOAD FILES
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| − | <h3> Wiki template information </h3>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <h3> Editing your wiki </h3>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | <a href="https://2018.igem.org/wiki/index.php?title=Team:Rheda_Bielefeld&action=edit">
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| − | EDIT PAGE
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| − | <h3>Tips</h3>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | <h3>Inspiration</h3>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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