Difference between revisions of "Team:NEFU China/Model1"

 
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<head>
 
<head>
 
<meta charset="utf-8">
 
<meta charset="utf-8">
−
<title>Untitled Document</title>
+
<title>Model 1</title>
−
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&amp;action=raw&amp;ctype=text/css"  rel="stylesheet" type="text/css">
+
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
 +
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 +
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-model1&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
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<style>
 
<style>
  
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     margin-left: 0px;
 
     margin-left: 0px;
 
}
 
}
−
 
+
#menu li ul li:hover ul{
 +
    background:rgba(0,0,0,0.75)!important;
 +
}
 +
li#mainlevel_01 a {
 +
    color: #FFE5B5!important;
 +
 +
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li#mainlevel_01 a:hover {
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
li#mainlevel_06 a {
 +
    color: #FFE5B5!important;
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 +
li#mainlevel_06 a:hover {
 +
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 +
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 +
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 +
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 +
    color: #FFE5B5!important;
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li#mainlevel_08 a:hover {
 +
    font-size:30px!important;
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    text-shadow:0px 0px 8px #FFE5B5,
 +
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 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
#menu li ul li ul li a:hover {
 +
    color: rgba(0,223,252,1);
 +
    border-top: dotted 1px rgba(255,255,255,0.91);
 +
    border-bottom: dotted 1px rgba(255,255,255,0.91);
 +
    background: rgba(0,223,252,.02);
 +
}
 +
#nav .mainlevel a {
 +
    color: black;
 +
    text-decoration:none;
 +
    line-height:32px;
 +
    display:block;
 +
    padding:0 5px;
 +
    font-size: 25px!important;
 +
font-family: 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif, 'Apple Color Emoji', 'Segoe UI Emoji', 'Segoe UI Symbol', 'Noto Color Emoji' !important;
 +
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 +
.layer-bottom {
 +
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 +
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 +
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 +
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#foot-title h3{
 +
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 +
}
 
</style>
 
</style>
 
</head>
 
</head>
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<body>
 
<body>
 
<!--menu-->
 
<!--menu-->
−
<div id="menu">
+
 
−
<li id="nav">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
+
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
−
<a class="menu1" href="#" style="text-align: right;">☰</a>
+
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 +
 
<ul class="firstmenu" style="float: left">
 
<ul class="firstmenu" style="float: left">
 
 
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<ul id="sub_02">
 
<ul id="sub_02">
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION &amp; DESIGN</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Design" target="_self">DESIGN</a></li>
+
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODE BOOK</a></li>
−
<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODING BOOK</a></li>
+
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
<li class="mainlevel" id="mainlevel_03">
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic parts"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENTS</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic parts" target="_self">BASIC PARTS</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite parts" target="_self">COMPOSITE PARTS</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">INFORMATION DESTRUCTION</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">Pre-RNA SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Parts collection" target="_self">PARTS COLLECTION</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
−
  </ul>
+
  <hr>
−
  </li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
−
      <li class="mainlevel" id="mainlevel_04">
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
+
−
  <ul id="sub_04">
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
+
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
 +
 
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <ul id="sub_05">
 
  <ul id="sub_05">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">MODEL1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">MODEL2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">KILLING MODEL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <ul id="sub_08">
 
  <ul id="sub_08">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Human_Practices" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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</li>
 
</li>
 
</div>
 
</div>
 +
<div class="layer-bottom">
 +
 +
<canvas id="canvas" style="background:#000000"></canvas>
 +
 +
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 2500;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 +
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
context.fillStyle = "rgba(0,0,0,0.2)";
 +
context.fillRect(0,0,W,H);
 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
for(var i=0;i<colunms/2;i++){
 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 +
</script>
 +
 +
           
 +
 +
</div>
 +
<header id="home-banner">
 +
    <div class="row banner">
 +
        <div class="banner-text">
 +
         
 +
           
 +
        </div>
 +
    </div>
 +
</header>
 +
 +
<section id="resume" style="box-shadow: inset 0px 15px 15px -15px cyan">
 +
<div class="row introduction">
 +
<div class="three columns header-col" style="">
 +
            <h1><span>intorduction</span></h1>
 +
        </div>
 +
        <div class="nine columns main-col">
 +
            <div class="row item">
 +
<div class="twelve columns">
 +
<br><br>
 +
                    <p style="font-size: 26px!important;">
 +
 +
Based on the procedure of our case, we hypothesized that the duration of induced spy yeast ( the information carrying yeast, designated as "spy yeast" ) death was relevant to the initial amounts of two types of yeast and production of secreted α factor. The duration of spy yeast death was proportional to the expression of Bax gene, while Bax gene expression depended on the activation efficiency of the promoter Fig2c. Thus, we should eventually determine whether the transcription efficiency of the Fig2c promoter is directly relevant to these three parameters. To verify the hypothesis, we used  correlation analysis to calculate the correlation <br>&lt;&gt;coefficients.<br>
 +
        Coefficients is :<br>
 +
                    <img src="https://static.igem.org/mediawiki/2018/5/53/T--NEFU_China--model1_f1.png"><br>
 +
 +
The data obtained from the wet laboratory, starting efficiency and initial alpha dosage of promoter Fig2c, were calculated correlation coefficient and fitted into a curve below.
 +
</p>
 +
</div>
 +
            </div>
 +
 +
        </div>
 +
      </div>
 +
  <hr>
 +
      <div class="row results">
 +
        <div class="three columns header-col">
 +
            <h1><span>RESULTS</span></h1>
 +
        </div>
 +
 +
        <div class="nine columns main-col">
 +
            <div class="row item">
 +
              <div class="twelve columns">
 +
  <br><br>
 +
<p style="font-size: 26px!important;">
 +
 +
  The result is as followed:</p><p style="
 +
font-size: 26px!important;
 +
"><br>
 +
Result 1: Correlation Coefficient of efficiency promoter Fig2c and initial dosage of killing yeast is below, and we find out their coefficient have strong correlation which verify our hypothesize is right. </p><p>
 +
</p><div align="center">
 +
    <span style="font-size: 100px;text-align: center;"></span>
 +
<img src="https://static.igem.org/mediawiki/2018/9/90/T--NEFU_China--model1_f2.png" style="width:600px;"><div>
 +
<br>
 +
<span style="
 +
font-size: 24px!important;
 +
    ">Result 2: Fitting into curve of two correlation coefficient.</span><br><br><br>
 +
<span style="text-align: left;"> </span>
 +
    <img src="https://static.igem.org/mediawiki/2018/0/0f/T--NEFU_China--model1-f3.png" style="width: 595px;"><br><br><br>
 +
  <p></p></div></div>
 +
</div>
 +
            </div>
 +
 +
        </div>
 +
      </div>
 +
<hr>
 +
 +
</section>
 
</body>
 
</body>
 +
 
</html>
 
</html>

Latest revision as of 13:08, 9 November 2018

Model 1

intorduction



Based on the procedure of our case, we hypothesized that the duration of induced spy yeast ( the information carrying yeast, designated as "spy yeast" ) death was relevant to the initial amounts of two types of yeast and production of secreted α factor. The duration of spy yeast death was proportional to the expression of Bax gene, while Bax gene expression depended on the activation efficiency of the promoter Fig2c. Thus, we should eventually determine whether the transcription efficiency of the Fig2c promoter is directly relevant to these three parameters. To verify the hypothesis, we used correlation analysis to calculate the correlation
<>coefficients.
Coefficients is :

The data obtained from the wet laboratory, starting efficiency and initial alpha dosage of promoter Fig2c, were calculated correlation coefficient and fitted into a curve below.

RESULTS



The result is as followed:


Result 1: Correlation Coefficient of efficiency promoter Fig2c and initial dosage of killing yeast is below, and we find out their coefficient have strong correlation which verify our hypothesize is right.


Result 2: Fitting into curve of two correlation coefficient.