Difference between revisions of "Team:NEFU China/Model1"

 
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<ul id="sub_02">
 
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<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
 
<li><a href="https://2018.igem.org/Team:NEFU_China/Background" target="_self">BACKGROUND</a></li>
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<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION</a></li>
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<li><a href="https://2018.igem.org/Team:NEFU_China/Description" target="_self">DESCRIPTION &amp; DESIGN</a></li>
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<li><a href="https://2018.igem.org/Team:NEFU_China/Design" target="_self">DESIGN</a></li>
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<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODE BOOK</a></li>
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<li><a href="https://2018.igem.org/Team:NEFU_China/Coding book" target="_self">CODING BOOK</a></li>
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  </ul>
 
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<li class="mainlevel" id="mainlevel_03">
 
<li class="mainlevel" id="mainlevel_03">
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  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENT</a>
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  <a href="https://2018.igem.org/Team:NEFU_China/Demonstrate"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENTS</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">INFORMATION DESTRUCTION</a></li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">Pre-RNA SPLICING</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Model1" target="_self">CORRESPONDING COEFFICIENT</a></li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">FIGHTING MODEL</a></li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Model2" target="_self">KILLING MODEL</a></li>
 
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
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  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Human_Practices"><img id="humanpractice" src="https://static.igem.org/mediawiki/2018/9/91/T--NEFU_China--_HUMANPRACTICE.png">HUMAN PRACTICE</a>
 
  <ul id="sub_08">
 
  <ul id="sub_08">
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Human_Practices" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Gold_integrated" target="_self">GOLD INTEGRATED</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Silver" target="_self">SILVER</a></li>
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            <h1 class="responsive-headline" style="color: cyan!important;font-size: 80px;background-color:rgb(0,0,0,0.85);">Corresponding Coefficient</h1>
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             <h1><span>intorduction</span></h1>
 
             <h1><span>intorduction</span></h1>
 
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                    <p style="font-size: 26px!important;">
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Based on the procedure of our case, we hypothesized that the duration of induced spy yeast death was relevant to the initial amounts of two types of yeast and production of secreted α factor. The duration of spy yeast death was proportional to the expression of Bax gene, while Bax gene expression depended on the activation efficiency of the promoter Fig2c. Thus, we should eventually determine whether the transcription efficiency of the Fig2c promoter is directly relevant to these three parameters. To verify the hypothesis, we used  correlation analysis to calculate the correlation coefficients.<br>
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 +
Based on the procedure of our case, we hypothesized that the duration of induced spy yeast ( the information carrying yeast, designated as "spy yeast" ) death was relevant to the initial amounts of two types of yeast and production of secreted α factor. The duration of spy yeast death was proportional to the expression of Bax gene, while Bax gene expression depended on the activation efficiency of the promoter Fig2c. Thus, we should eventually determine whether the transcription efficiency of the Fig2c promoter is directly relevant to these three parameters. To verify the hypothesis, we used  correlation analysis to calculate the correlation <br>&lt;&gt;coefficients.<br>
 
        Coefficients is :<br>
 
        Coefficients is :<br>
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                     <img src="https://static.igem.org/mediawiki/2018/5/53/T--NEFU_China--model1_f1.png"><br>
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                     <img src="https://static.igem.org/mediawiki/2018/5/53/T--NEFU_China--model1_f1.png"><br>  
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Suppose ρ is correlation coeeficients, σ<sub>1</sub>σ<sub>2</sub>is quantity of α yeast variance and the expression of Bax gene ε variance.<br>
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The result is as followed: it also verify our hypothesize is right.<br>
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The data obtained from the wet laboratory, starting efficiency and initial alpha dosage of promoter Fig2c, were calculated correlation coefficient and fitted into a curve below.
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    <span style="font-size: 30px;text-align: center;">Correlation</span>
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</p>
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<img src="https://static.igem.org/mediawiki/2018/9/90/T--NEFU_China--model1_f2.png">
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**.has significiant positive correlation on 0.01
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    <img src="https://static.igem.org/mediawiki/2018/0/0f/T--NEFU_China--model1-f3.png">
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             <h1><span>RESULTS</span></h1>
 
             <h1><span>RESULTS</span></h1>
 
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  The result is as followed: it also verify our hypothesize is right.
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<p style="font-size: 26px!important;">
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  The result is as followed:</p><p style="
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font-size: 26px!important;
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"><br>
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Result 1: Correlation Coefficient of efficiency promoter Fig2c and initial dosage of killing yeast is below, and we find out their coefficient have strong correlation which verify our hypothesize is right. </p><p>
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</p><div align="center">
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    <span style="font-size: 100px;text-align: center;"></span>
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<img src="https://static.igem.org/mediawiki/2018/9/90/T--NEFU_China--model1_f2.png" style="width:600px;"><div>
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<br>
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<span style="
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font-size: 24px!important;
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    ">Result 2: Fitting into curve of two correlation coefficient.</span><br><br><br>
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<span style="text-align: left;"> </span>
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    <img src="https://static.igem.org/mediawiki/2018/0/0f/T--NEFU_China--model1-f3.png" style="width: 595px;"><br><br><br>
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<h1>Userfull links</h1>
 
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<h2>
 
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<a href="https://2018.igem.org/Team:NEFU_China">Home</a>
 
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&nbsp;&nbsp;&nbsp;
 
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<a href="https://2018.igem.org/Team:NEFU_China/Model">Model</a>
 
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                        &nbsp;&nbsp;
 
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                        <a href="https://2018.igem.org/Team:NEFU_China/Description">Project</a>
 
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&nbsp;&nbsp;
 
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<a href="https://2018.igem.org/Team:NEFU_China/Software">Software</a>
 
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    &nbsp;&nbsp;
 
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    </h2>
 
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<h2>
 
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<a href="https://2018.igem.org/Team:NEFU_China/Basic_Part">Parts</a>
 
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&nbsp;&nbsp;&nbsp;&nbsp;
 
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<a href="https://2018.igem.org/Team:NEFU_China/Members">Teams</a>
 
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&nbsp;
 
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<a href="https://2018.igem.org/Team:NEFU_China/Lock_Key">Results</a>
 
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&nbsp;
 
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<a href="https://2018.igem.org/Team:NEFU_China/Notebook">Notebook</a>
 
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                        &nbsp;&nbsp;
 
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</h2>
 
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<h2>
 
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<a href="https://2018.igem.org/Team:NEFU_China/Human_Practices">Human &nbsp;Practice</a>
 
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</h2>
 
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<h1>Follow us</h1>
 
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<img alt="facebook" src="https://static.igem.org/mediawiki/2018/b/b5/T--NEFU_China--facebook.png">
 
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&nbsp;&nbsp;&nbsp;
 
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        <img alt="twitter" src="https://static.igem.org/mediawiki/2018/3/36/T--NEFU_China--twitter.png">
 
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&nbsp;&nbsp;&nbsp;
 
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<img alt="wehchat" src="https://static.igem.org/mediawiki/2018/c/ca/T--NEFU_China--wechat.png">
 
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        <br>
 
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        <img alt="facebook" src="https://static.igem.org/mediawiki/2018/b/b5/T--NEFU_China--facebook.png">
 
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&nbsp;&nbsp;&nbsp;
 
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        <img alt="twitter" src="https://static.igem.org/mediawiki/2018/3/36/T--NEFU_China--twitter.png">
 
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<img alt="wehchat" src="https://static.igem.org/mediawiki/2018/c/ca/T--NEFU_China--wechat.png">
 
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<h1>Contact us</h1>
 
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<h3>iGEM-NEFU_China2018</h3>
 
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<h3>Email: hexinglu@nefu.edu.cn</h3>
 
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<h3>No.26 Hexing Road, Xiangfang <br>District, Harbin, Heilongjiang <br>Province 150000</h3>
 
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Latest revision as of 13:08, 9 November 2018

Model 1

intorduction



Based on the procedure of our case, we hypothesized that the duration of induced spy yeast ( the information carrying yeast, designated as "spy yeast" ) death was relevant to the initial amounts of two types of yeast and production of secreted α factor. The duration of spy yeast death was proportional to the expression of Bax gene, while Bax gene expression depended on the activation efficiency of the promoter Fig2c. Thus, we should eventually determine whether the transcription efficiency of the Fig2c promoter is directly relevant to these three parameters. To verify the hypothesis, we used correlation analysis to calculate the correlation
<>coefficients.
Coefficients is :

The data obtained from the wet laboratory, starting efficiency and initial alpha dosage of promoter Fig2c, were calculated correlation coefficient and fitted into a curve below.

RESULTS



The result is as followed:


Result 1: Correlation Coefficient of efficiency promoter Fig2c and initial dosage of killing yeast is below, and we find out their coefficient have strong correlation which verify our hypothesize is right.


Result 2: Fitting into curve of two correlation coefficient.