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<img class="ill-med" src="https://static.igem.org/mediawiki/2018/7/77/T--VIT_Vellore--ill3.png" alt="Illustration 3"/> | <img class="ill-med" src="https://static.igem.org/mediawiki/2018/7/77/T--VIT_Vellore--ill3.png" alt="Illustration 3"/> | ||
− | <p class="ill-description">Repressor molecule LexA is produced in order to repress the expression of the biobrick at | + | <p class="ill-description">Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH</p> |
</div> | </div> | ||
<div id="ill-4" class="horizontal ill-container"> | <div id="ill-4" class="horizontal ill-container"> |
Revision as of 03:20, 7 December 2018
togglepH
VIT Vellore
We use a plasmid system consisting of a composite part with two promoters
This part gets activated at ~pH 9.5 and produces the necessary proteins to increase the surrounding pH
Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH
LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process