Difference between revisions of "Team:VIT Vellore"

Line 34: Line 34:
 
     <div id="ill-3" class="horizontal ill-container">
 
     <div id="ill-3" class="horizontal ill-container">
 
         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/7/77/T--VIT_Vellore--ill3.png" alt="Illustration 3"/>
 
         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/7/77/T--VIT_Vellore--ill3.png" alt="Illustration 3"/>
         <p class="ill-description">Repressor molecule LexA is produced in order to repress the expression of the biobrick at high pH</p>
+
         <p class="ill-description">Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH</p>
 
     </div>
 
     </div>
 
     <div id="ill-4" class="horizontal ill-container">
 
     <div id="ill-4" class="horizontal ill-container">

Revision as of 03:20, 7 December 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 9.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process