Difference between revisions of "Team:VIT Vellore"

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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/1/13/T--VIT_Vellore--ill2.png" alt="Illustration 2"/>
 
         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/1/13/T--VIT_Vellore--ill2.png" alt="Illustration 2"/>
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         <p class="ill-description">This part gets activated at ~pH 9.5 and produces the necessary proteins to increase the surrounding pH</p>
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         <p class="ill-description">This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH</p>
 
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Revision as of 03:22, 7 December 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process